The present application relates to anti-PD-L1 antibodies and their use to detect PD-L1 in a sample from a subject. In some embodiments, the subject has been treated with a therapeutic anti-PD-L1 antibody and an anti-PD-L1 described herein does not compete for binding to PD-L1 with the therapeutic anti-PD-L1 antibody. In some embodiments, the anti-PD-L1 antibody is linked to a detectable moiety, such as a fluorophore and the anti-PD-L1 antibody is used to detect PD-L1 in a subject using flow cytometry.

One variation of a system includes a cartridge comprising: a substrate; a sample well integrated into the substrate, defining an upper opening and a lower opening, and configured to receive a test solution comprising a user sample and an amount of a fluorescent probe configured to bind with a target bioparticle to form a target complex; a filter membrane extending across the lower opening and defining a network of pores configured to convey fluid from the sample well and prevent passage of the target complex through the filter membrane. The system further includes a reader comprising: a housing; a cartridge receptacle configured to receive the cartridge; an excitation source configured to illuminate a detection region within the housing; and a detector defining a field of view intersecting the detection region and configured to detect a signal generated by fluid in the sample well and representing presence of the target bioparticle.

Provided is a novel fluorescent probe.

A compound of the following general formula (I) or a salt thereof.

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There is provided a biological substance detection chip having high detection accuracy. The present technology provides a biological substance detection chip which is composed of a plurality of pixels in which the pixel includes at least a holding surface on which a biological substance is held and a photoelectric conversion unit that is provided below the holding surface and provided on a semiconductor substrate, wherein a partition wall made of a conductor is provided between the pixels on the holding surface. In addition, the present technology provides a biological substance detection device and a biological substance detection system using the biological substance detection chip.

A flow cytometer can include: at least one light emitter configured to emit light in a light path; a rectangular flow cell having flow cell width that is substantially lateral to the light path and a flow cell depth that is longitudinal to the light path, wherein the light path has an interrogation width at the flow cell that is narrower than the flow cell width; and a spherical reflector positioned adjacent to the rectangular flow cell and having a concave reflective surface that has a reflective direction that is positioned substantially orthogonal with the light path such that reflected light is reflected along a reflected path that is substantially orthogonal with the light path. At least one light absorbing member is positioned at least partially around the reflected path to absorb reflected light at an angle to the reflected path.

Systems, devices, and methods for producing an optimized phase mask for use in a single-molecule orientation localization microscopy (SMOLM) imaging system are disclosed.

Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.

Systems and methods for measuring short wave infrared fluorescence and autofluorescent signals are disclosed. In some embodiments, for example, a method may include exposing a portion of tissue that does not include a fluorescent probe to an excitation source of the tissue, wherein at least a portion of the tissue has an autofluorescence spectrum which includes wavelengths greater than 900 nm, and imaging the tissue with a detector that is sensitive to electromagnetic radiation with wavelengths greater than or equal to 900 nm. In certain other embodiments, a system comprises a fluorescent probe including a fluorescent component attached to a carrier, an excitation source, and a detector that detects a tail portion of the fluorescence of the fluorescent component. Methods associated with such a system are also disclosed.

The invention refers to a method and a device for the phenotypic detection of carbapenemases and carbapenemase producers by adding a substrate of general formula A-(L)-M.sub.1-(X)—Z, where M.sub.1 is a carbapenem backbone, A or Z is a quencher, the other one of the two, Z or A, is a fluorophore, L is an optional linker, X is an optional leaving group for linking Z to the carbapenem backbone, and Z is an optional leaving group, to a sample suspected of containing such carbapenemase producers and/or carbapenenmases. The invention further refers to a method for the phenotypic detection of resistant bacteria, in particular 3MRGN or 4MRGN, by releasing the enzymes of a bacterial culture into a lysate during lysis and then subjecting the lysate, as the sample to be analyzed, to an aforementioned method in order to phenotypically detect the presence of resistance-conferring carbapenemases.

A sensing device includes a first sensor configured to capture a first analyte in a fluid medium and to generate a first signal in response to capturing the first analyte. The sensing device also includes a second sensor configured to capture a second analyte in the fluid medium and to generate a second signal in response to capturing the second analyte, where the second analyte is different from the first analyte. The sensing device further includes a detector configured to collect the first and second signals to provide a total signal and to calculate a total concentration of the first and the second analyte in the fluid medium based on the total signal.