An excitation light source emits excitation light to a target sample. An image sensor includes pixels arranged one-dimensionally or two-dimensionally, and receives measurement light from the sample according to the excitation light. A polarization selector arranged between the sample and image sensor includes pixels arranged one-dimensionally or two-dimensionally. Each pixel receives a corresponding portion of the measurement light, selects light having a polarization direction that corresponds to a driving signal applied to the pixels, and supplies this light to the image sensor. A measurement control unit supplies the cyclic driving signal having a first period T.sub.1 and acquires data I.sub.1, I.sub.2, I.sub.3, and I.sub.4 from each pixel of the image sensor for each exposure time segment T.sub.2=T.sub.1/4 obtained by dividing the first period T.sub.1 by 4.

The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

A system and a method directed to a monitoring system of semiconductor processing chambers is provided. In particular, monitoring of any chemical formation on a chamber and a wafer of a semiconductor processing chamber using in-situ laser induced fluorescence is provided. The monitoring system and method detect issues before they become a problem for the semiconductor processing chambers by providing diagnosis on chamber health and mechanisms for associated process shifts with a faster turnaround time.

Sub-diffraction limited fluorescent images using a fiber-based stimulated emission depletion (STED) microscope are reported. Both excitation and depletion beams are transported through polarization-maintaining fiber and a lateral resolution of 100 nm has been achieved.

The present invention relates to technical field of C12N15/115, and particularly relates to an aptamer-based fluorescence polarization detection method for extracellular vesicles (EVs) and its application. The method comprises the following steps: S1. immobilizing EVs by interacting with antibodies against surface-biomarker proteins of EVs or surface cancer markers thereof; rapidly washing them to remove free EVs, proteins, membrane fragments, and lipids; S2. respectively adding aptamers matched with EV markers or cancer cell markers therein and cultivating them the aptamers are fluorescently labeled; S3. performing fluorescence polarization detection on the products from Step S2 to achieve qualitative and quantitative analysis of EVs secreted by cancer cells. This invention can specifically detect extracellular vesicles secreted by cancer cells in blood, and detection process is not interfered with by free tumor marker proteins, tumor cell membrane fragments, or tumor cell extracellular vesicle membrane fragments in blood. The detection results are accurate and effective.

Provided is an analysis method for determining at least any one of: presence or absence of a target substance; and a concentration of the target substance through use of a reagent that reacts with the target substance, the analysis method including: a loading step of loading: a sample containing the target substance; a hydrophilic polymer that reacts with a silanol group of a glass vessel; and the reagent into the glass vessel; a reaction step of causing the target substance and the reagent to react with each other to provide a reaction liquid; and an analysis step of determining at least any one of: the presence or absence of the target substance; and the concentration of the target substance in the reaction liquid, wherein the loading step includes loading the reagent after or simultaneously with the loading of the hydrophilic polymer.

A multiphoton fluorescence anisotropy microscopy live cell imaging system and method to measure and map drug-target interaction in real lime at subcellular resolution. Proposed modality enables a direct measurement of drug/target binding in vivo, high-resolution spatial and temporal snapping of bound and unbound drug distribution, and presents an versatile tool to enhance understanding of drug activity. Application of tire system to measurement of intracellular target engagement of the chemotherapeutic Olaparib, a poly(ADP-ribose) polymerase inhibitor, in live cells and within a tumor in vivo.

The present invention pertains to a surface plasmon enhanced fluorescence analysis device and a surface plasmon enhanced fluorescence measurement method which use GC-SPFS and make it possible to detect a substance to be detected with high sensitivity. This surface plasmon enhanced fluorescence measurement device has: a light source for irradiating the diffraction grating of a chip with excited light; a polarizer for removing linearly polarized light from fluorescent light emitted from a fluorescent substance on the diffraction grating; and a photodetector for detecting the linearly polarized light removed by the polarizer.

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.