In various embodiments, a lighting device is provided. The lighting device includes a phosphor volume for at least partial wavelength conversion of primary light into secondary light, a primary light semiconductor light source for irradiating the phosphor volume with primary light, a measurement light generating arrangement for generating measurement light having a spectral composition outside the primary light and the secondary light, a measurement light detector sensitive to the measurement light, and a measurement light filter, which is fixedly connected to the phosphor volume and is optically arranged between the measurement light generating arrangement and the measurement light detector.

A non-invasive measurement of biological tissue reveals information about the function of that tissue. Polarized light is directed onto the tissue, stimulating the emission of fluorescence, due to one or more endogenous fluorophors in the tissue. Fluorescence anisotropy is then calculated. Such measurements of fluorescence anisotropy are then used to assess the functional status of the tissue, and to identify the existence and severity of disease states. Such assessment can be made by comparing a fluorescence anisotropy profile with a known profile of a control.

A method for identifying the impact on data, such as experimental data, of interfering effects, such as unwanted auto-fluorescence, fluorescence quenching, and fluorescent-sample deterioration, whether or not the data fulfill certain criteria with respect to a threshold indicative of the interfering effects.

Measurement device for the detection and/or characterization of fluid-borne particles featuring a laser positioned for emitting pulses of laser light polarized in a first direction, in a measurement volume of a fluid flow path, each pulse having a pulse duration, and a means for directing the laser pulses polarized in a second different direction of polarization in the measurement volume. An optical spectrometer captures fluorescent light emitted by individual fluid-borne particles and measures the intensity of at least one wavelength at a sampling rate of at least three samples per pulse. The means for directing are configured to direct laser light polarized in the second direction each time a pulse of laser light crosses the measurement volume, the time delay between the two crossings being longer than the pulse duration and shorter than a travel time of the fluid in the measurement volume.

Sub-diffraction limited fluorescent images using a fiber-based stimulated emission depletion (STED) microscope are reported. Both excitation and depletion beams are transported through polarization-maintaining fiber and a lateral resolution of 100 nm has been achieved.

According to one embodiment of the present invention, a structure includes: a substrate having a patterned surface of one or more binding sites; and a molecular shape made by a polynucleotide platform having a shape corresponding to a shape of a binding site of the one or more binding sites, the molecular shape being bound to one of the one or more binding sites.

Device (1) for characterizing a substance (2) capable of emitting a photoluminescence radiation (Rp) in a first spectral range, the device (1) comprising: an electroluminescent component (3), at least semi-transparent in the first spectral range, and comprising first and second opposite surfaces (30, 31), the electroluminescent component (3) being suitable for emitting an excitation radiation (Re.sub.1) outgoing from the first surface (30), emitted in a first spectral range according to a circular polarization state; the excitation radiation (Re.sub.1) outgoing from the first surface (30) being able to pass through the electroluminescent component (3), after being reflected, and exit from the second surface (31); a polarization filter (4), arranged to filter the excitation radiation (Re.sub.2) outgoing from the second surface (31), and suitable for modifying the circular polarization state so as to obtain an extinguishing of the excitation radiation (Re.sub.2) outgoing from the second surface (31) of the electroluminescent component (3); a detector (5), arranged to detect the photoluminescence radiation (Rp) outgoing from the polarization filter (4).

An analyzing system includes a fluidic stream that guides an analyte in a detection area where the analyte emits or transmits light. One or more polarizing elements polarize the light into respective two or more different polarization components. One or more optical detectors receive the respective two or more polarization components and generate respective at least two signals in response. A processor is coupled to the optical detectors and configured to determine the polarization status of the light from the object based on the signals.

An image reconstruction method includes capturing a reference image of the specimen and capturing a set of original images based on the reference image. The method includes generating a set of analyzed images based on the set of original images by determining an intensity distribution for each pixel of each original image of the set of original images and combining the intensity distribution at each pixel location across the set of original images into an intermediate image. The method includes, identifying an object in the intermediate image. In response to identifying the object in the intermediate image, determining an intensity value of the object in each original image of the set of original images and generating an improved image of the object based on the determined intensity value of the object. The method includes generating a final image including the improved image of the object and displaying the final image.

A non-invasive measurement of biological tissue reveals information about the function of that tissue. Polarized light is directed onto the tissue, stimulating the emission of fluorescence, due to one or more endogenous fluorophors in the tissue. Fluorescence anisotropy is then calculated. Such measurements of fluorescence anisotropy are then used to assess the functional status of the tissue, and to identify the existence and severity of disease states. Such assessment can be made by comparing a fluorescence anisotropy profile with a known profile of a control.