The present disclosure relates to an in vivo fluorescent or radioactive probe represented by a compound of formula I which is capable of longitudinal imaging of inducible nitric oxide synthase (iNOS) expression in living cells and living animals on a real time basis. The probe of the present disclosure can exhibit specific and high affinity binding to the iNOS enzyme with reduced enzyme inhibitory property and also enables longitudinal monitoring of iNOS expression along with its activity or NO production in a same experimental subject throughout the progression of a physiological or disease process without employing separate subjects as controls and experimental. The present disclosure further provides a rapid and inexpensive real time method for visualizing iNOS expression and its activity in living cells and living animals precisely, conveniently and reversibly along with simultaneous in vivo imaging of its catalytic product, nitric oxide (NO) in live physiological settings.

The present invention provides a wide-area sample-based reader design which serves as a diagnostic detection device for bio-particles.

High photoluminescence, high stability, inorganic perovskite compounds comprising an alkali metal selected from potassium (K), rubidium (Rb), and cesium (Cs); copper (Cu); and at least one halogen selected from chlorine (Cl), bromine (Br), and iodine (I). The perovskites may be free of lead (Pb). The inorganic perovskite compound may be used in an optoelectronic device. The optoelectronic device optionally contains a phosphor such as a blue-emitting phosphor. The inorganic perovskite compound may be used as an anti-counterfeiting nanotaggant applied on or within an object that susceptible to counterfeiting to enable confirmation of an authentic object.

In-situ fluorimeters and methods and systems for collecting and analyzing sensor data to predict water source contamination are provided. In one embodiment, a method is provided that includes receiving sensor data regarding a water source. Changepoints may then be calculated within the sensor data and the sensor data may be split into intervals at the changepoints. A machine learning model may then be used to classify the intervals and a predicted contamination event for the water source may be identified based on the classified intervals. In another embodiment, an in-situ fluorimeter is provided. The in-situ fluorimeter comprises one or more UV LEDs centered around a pre-set excitation wavelength (e.g., a TLF excitation wavelength), a bandpass filter, a lens, a photodiode system, a machine learning platform; and an alarm triggered by contamination events, wherein the alarm is calibrated through the machine learning system.

Examples relate to a microscope, and to an apparatus, method and computer program for a microscope. The microscope comprises a light emission module for providing illumination for a sample of organic tissue in a plurality of wavelength bands. The microscope comprises one or more imaging sensor modules configured to independently sense light in a plurality of mutually separated wavelength bands of the plurality of wavelength bands. The microscope comprises a processing module configured to control the light emission module such, that in a first operating mode light in a first subset of the plurality of wavelength bands is emitted towards the sample of organic tissue, and that in a second operating mode light in a second subset of the plurality of wavelength bands is emitted towards the sample of organic tissue. The first and second subset of wavelength bands are at least partially different. The processing module is configured to use the one or more imaging sensor modules to perform reflectance imaging and fluorescence imaging in each of the plurality of mutually separated wavelength bands based on the light emitted in the first and second operating modes.

An image acquire device comprising: an irradiator configured to irradiate an undyed tissue with excitation light; an image sensor configured to acquire a third harmonic image of the undyed tissue based on light generated in third harmonic generation caused by interaction between the undyed tissue and the excitation light.

Cell counting device A cell counting device and a method of using a cell counting device are disclosed. The cell counting device comprises a chamber for receiving a sample, at least one light source to emit light towards a section of the chamber. The section of the chamber comprises a sub-sample of the sample. The cell counting device also comprises a light detector to receive a light emitted from the section of the chamber and to generate an electronic signal associated with the received light, and a controller. The controller is configured to estimate the number of photoactive cells in the sample by calculating the distribution of variable fluorescence [F.sub.v] values of a predetermined number of sub-samples about the mean F.sub.v value.

An apparatus for detecting an optical signal emissions includes signal transmission fibers. Each fiber includes cores having the same spatial core arrangement at each end. The first ends are configured to be optically coupled to the signal emission sources. Each fiber is configured to transmit an optical signal between the first end and the second. The apparatus can also include a frame assembly securing the first ends of the fibers in a first spatial fiber arrangement corresponding to a spatial arrangement of the signal emission sources. The frame assembly can also secure the second ends of the fibers in a second spatial fiber arrangement different from the first spatial fiber arrangement. The apparatus can also include at least one signal detector configured to be optically coupled to the second ends of the fibers, and configured to detect an optical signal emitted by each signal emission source.

Hardware and control software for use in the field of digital imaging and spectroscopy. More particularly, a hardware and software system that simultaneously measures electromagnetic energy as quantities of photons in distinct wavelength regions across the ultraviolet, visible, and infrared spectrum. The system records the measurements as digital data and employs a processor (preferably a programmable processor) that executes processing steps to enhance the spatial and spectral fidelity of the recorded signals. More specifically, the electro-optical sensor hardware is engineered to maximize the light collection efficiency, especially for low light intensities, by using multiple detectors, each of which is optimized individually to maximize its sensitivity to specific wavelength regions of interest. The detector system also employs a variable amplification process that is dependent on the signal intensity so that low signals can be increased for better detection while high signals are amplified less to stay within the dynamic range of the optical sensor that is used to convert the analog signal to a digital value. Solutions to existing problems of low light detection are provided as are new capabilities for data collection and analysis in previously undetectable low signal regimes. The systems and methods are applicable to a broad array of imaging applications in diverse fields from biomedical imaging to astronomy and remote sensing.

A portable ring-type fluorescence optical system for observing microfluidic channel and an operating method thereof are disclosed. The portable ring-type fluorescence optical system includes a photographic chip, a first polarizer, an objective lens, a ring-type fluorescent light source, a biological sample on a microfluidic chip, a second polarizer and a bottom illumination light source arranged in order from top to bottom. The ring-type fluorescent light source is used to generate a ring-type fluorescent light to the biological sample on the microfluidic chip. The objective lens is used to magnify a fluorescent image of the biological sample on the microfluidic chip to focus on the photographic chip. The first polarizer disposed under the photographic chip and the second polarizer disposed under the biological sample form a non-zero angle to each other to block reflected lights that the biological sample reflects the lights emitted by the bottom illumination light source.