To provide a test substance measurement method and a test substance measurement kit adapted to improve the accuracy of the measurement of a test substance. A test substance measurement kit includes: fluorescent particles which are modified with a first binding substance having specific bindability to a test substance; non-fluorescent particles which are modified with a second binding substance having no specific bindability to the test substance; and a substrate on which a first metal film to which a third binding substance having specific bindability to the test substance is fixed, and a second metal film to which a fourth binding substance having no bindability to the test substance, but having bindability to the first binding substance is fixed, and which has a smaller thickness than the first metal film are formed.

A system for the continuous detection of microbes of interest in waters, including: at least one biosensor tank having an inlet and an outlet for the waters to be monitored and containing nanoparticles functionalised with at least one antibody specific for the antigenic capture of a particular type of microbe present in the water, and the creation of networks that give rise to an agglutination reaction that creates turbidity in the water, captured by a spectrophotometric sensor or sensor for the measurement of the turbidity in the water; and a control unit for monitoring, logging, and processing the information provided by the spectrophotometric sensor or sensor for the measurement of turbidity; and for monitoring the operation of the system.

What is disclosed is a method for creating a database for determining a virtual reference value for a light transmission aggregometry measurement, comprising the following steps: a. providing platelet-rich plasma PRP (17) of a reference blood sample; b. performing a light transmission measurement with a first light wavelength and a second light wavelength (14), different from the first, on the PRP (17) of the reference blood sample; c. providing platelet-poor plasma PPP of the reference blood sample; d. performing a light transmission measurement on the PPP in order to determine a PPP reference value; e. assigning the measurement result of step d to the measurement results of step b in a database; f. repeating steps a to e for a plurality of reference blood samples.

The database obtained using this method makes it possible to determine a virtual reference value that is an excellent estimate of the PPP reference value. Once the database has been created, it is possible to determine virtual reference values of blood samples to be examined and perform LTA measurements using the virtual reference values, without the need to obtain PPP from the blood samples to be examined.

A system for processing sample entities includes a chamber including a surface having an array of measurement regions, wherein at least one measurement region comprises a first set of one or more electrodes and a second set of one or more electrodes, wherein the first set of electrodes is configured to measure a first characteristic of a sample entity when the sample entity is traversing the first set of electrodes, and wherein the second set of electrodes is configured to selectively retain the sample entity in the at least one measurement region based at least in part on the measured first characteristic and/or measure a second characteristic of the sample entity.

A method for determining the amount of specific analyte of a sample which may show interferences by photometric assays, wherein the analyte is quantified from the change in the optical signal of the reaction mixture after the interaction of the analyte with analyte specific reagents. Multiple calibration curves are generated for multiple wavelengths for the specific analyte. An interference test is performed simultaneously to the determination of the specific analyte, for quantifying the amount of interfering substances present in the sample. The amount of each interfering substances is compared to predetermined cut-off values. The optical signal for the specific analyte is measured in the reaction mixture at multiple wavelengths over the complete reaction time, and a calibration curve is selected depending on the interfering substances. The amount of specific analyte is quantified by comparison with the selected calibration curve for the chosen wavelengths.

Disclosed is an analyzing method, for analyzing a blood specimen, which includes: mixing a blood specimen and a thrombin-containing reagent to coagulate the blood specimen, and obtaining a coagulation waveform; obtaining a value of a parameter concerning differentiation of the coagulation waveform, based on the coagulation waveform; and obtaining information concerning an amount of antigen of fibrinogen based on the obtained value of the parameter.

A method is provided for of preparing a glass bead mixture using inert nanoparticles to improve flow rates of the glass beads for purposes of manufacturing an immunodiagnostic test element, such as a column agglutination test cassette. The immunodiagnostic test element includes a plurality of test columns including an aqueous reagent in each test column.

Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided. Also provided herein are instruments that are programmed to operate a cartridge that includes an array of electrodes for actuating a droplet and further includes an electrochemical species sensing region. The instrument may be used to analyse a sample in a cartridge that includes an array of electrodes for actuating a droplet and further includes a nanopore layer for detecting translocation of a tag/second binding member through nanopore. An instrument configured to operate a first cartridge that includes an array of electrodes for actuating a droplet and further includes an electrochemical species sensing region and a second cartridge that includes an array of electrodes for actuating a droplet and further includes a nanopore layer for detecting translocation of a tag/second binding member through nanopore is disclosed. An instrument configured to operate a cartridge that includes an array of electrodes for actuating a droplet, an electrochemical species sensing region, and a nanopore layer for detecting translocation of a tag/second binding member through nanopore is disclosed.

Hand-held optical thromboelastographic sensor and method of using the same for simultaneous assessment of multiple parameters of blood coagulation at a point-of-care. The sensor includes an optical system registering laser speckle intensities associated with portions of a blood sample delivered through a fluid switch to analysis chambers of a cartridge of the sensor, and data-processing circuitry programmed to derive the multiple parameters from speckle intensity. The circuitry may be part of a mobile device configured to operate without communication with a central server and/or data storage.

A blood coagulation analyzer and analyzing method perform following: (a) preparing a measurement specimen by dispensing a blood specimen and a reagent into a reaction container; (b) emitting light of a plurality of wavelengths to the measurement specimen in the reaction container, the wavelengths comprising a first wavelength for use in a measurement by a blood coagulation time method, and at least one of a second wavelength for use in a measurement by a synthetic substrate method and a third wavelength for use in a measurement by an immunoturbidimetric method; (c) detecting light of a plurality of wavelengths corresponding to the light emitted in (b), from the measurement specimen, by a light receiving element, and acquiring data corresponding to each wavelength; and (d) conducting an analysis based on the data corresponding to one of the wavelengths among the acquired data, and acquiring a result of the analysis.