A charge sensor element includes a charge collecting detector configured to generate an intensity signal indicative of an amount of charge at an internal charge sensor element node, an amplifier transistor that is electrically connected to the internal charge sensor element node and configured to amplify the intensity signal, and a reset transistor that is electrically connected to the internal charge sensor element node and configured to reset the intensity signal. The amplifier transistor or the reset transistor includes a front gate and a back gate that are configured to control the amplifier transistor or the reset transistor.

Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

A cell manipulation panel includes a pixel array defining multiple pixels, an insulating layer forming multiple vias, and a cell gap provided with a fluid medium having cells therein. Each pixel has a TFT and corresponds to a corresponding via. The TFT includes a gate electrode, a first electrode, and a second electrode partially exposed to the fluid medium through the corresponding via. For each pixel, in an operational mode, when the gate electrode is provided with an OFF signal and the first electrode is not grounded, the TFT is turned off, allowing one of the cells in the fluid medium to be captured in the corresponding via by a dielectrophoresis (DEP) force. When the gate electrode is provided with an ON signal and the first electrode is grounded, the TFT is turned on, and the second electrode is grounded to release the captured cell to the fluid medium.

A device for analysis of cells comprises: an active sensor area (104) presenting a surface for cell growth; a microelectrode array (102) comprising a plurality of pixels (110) in the active sensor area (104), wherein each pixel (110) comprises at least one electrode (120) at the surface, wherein each pixel (110) is configured to control the configuration of the pixel circuitry and set a measurement modality of the pixel; recording circuitry having a plurality of recording channels (130), wherein each pixel (110) is connected to a recording channel (130), wherein each recording channel (130) comprises a reconfigurable component (131), which is selectively controlled between being set to a first mode, in which the reconfigurable component (131) is configured to amplify a received pixel signal, and being set to a second mode, in which the reconfigurable component (131) is configured to selectively pass a frequency band of the received pixel signal.

The present disclosure provides a bio-field effect transistor (BioFET) and a method of fabricating a BioFET device. The method includes forming a BioFET using one or more process steps compatible with or typical to a complementary metal-oxide-semiconductor (CMOS) process. The BioFET device may include a substrate; a gate structure disposed on a first surface of the substrate and an interface layer formed on the second surface of the substrate. The interface layer may allow for a receptor to be placed on the interface layer to detect the presence of a biomolecule or bio-entity.

A semiconductor sensor-based near-patient diagnostic system and related methods.

Provided are biosensors, systems and related methods of using the biosensors and systems. The biosensor comprises a field-effect transistor (FET) having a crumpled geometry to effectively increase the detection sensitivity of a target molecule in an ionic solution. A FET having a crumpled semiconductor material channel can form a π-π interaction with single stranded DNA (ssDNA) for amplification detection applications. Increasing amount of ssDNA in an amplification reaction solution is incorporated into an amplified double stranded DNA, with increasing amplification, resulting in a lower amount of ssDNA primers. The FET is contacted with the amplified solution to electrically detect an amount of ssDNA primer in the amplified solution, thereby detecting amplification based on a decreased amount of ssDNA bound to the FET. Also provided are biosensors that can detect biomolecules more generally, such as protein, polypeptides, polynucleotides, or small molecules.

Methods, systems, and apparatuses for efficiently amplifying and detecting certain nucleic acid sequences from a population. The selected population can be further characterised, for example by sequencing. A method involves combined solution phase and solid phase amplification.

An organic electrochemical transistor, which includes a biologic detection layer and a catalytic layer, the latter being a composite material including noble metal nanoparticles and an organic conductive matrix. Also, a method for detection of a biological analyte wherein a biological fluid is contacted with such an organic electrochemical transistor.

In one embodiment, a device is described. The device includes a material defining a reaction region. The device also includes a plurality of chemically-sensitive field effect transistors have a common floating gate in communication with the reaction region. The device also includes a circuit to obtain respective output signals from the chemically-sensitive field effect transistors indicating an analyte within the reaction region.