The present invention provides a novel method of manufacturing germinated plant seeds suitable for producing a large amount of phytoalexin, a method of manufacturing raw material seeds for germination induction for use in manufacture of the above germinated plant seeds, an extract composition of the germinated plant seeds and a screening method for a plant seed candidate for use in producing a target substance. The method of manufacturing raw material seeds for germination induction comprises a pre-treatment step of maintaining plant seeds under atmosphere conditions of a carbon dioxide concentration of 400 ppm or more and/or an oxygen concentration of 19 vol % or less continuously for 5 hours or more.

A sample extraction device and a desorption device for use in gas chromatography (GC), gas chromatography-mass spectrometry (GCMS), liquid chromatography (LC), and/or liquid chromatography-mass spectrometry (LCMS) are disclosed. In some examples, the sample extraction device includes a lower chamber holding a sorbent. The sample extraction device can extract sample headspace gas from a sample vial by placing the sorbent inside the vial and creating a vacuum to increase recovery of low volatility compounds, for example. Once the sample has been collected, the sample extraction device can be inserted into a desorption device. The desorption device can control the flow of a carrier fluid (e.g., a liquid or a gas) through the sorbent containing the sample and into a pre-column and/or a primary column of a chemical analysis device for performing GC, GCMS, LC, LCMS, and/or some other chemical analysis process.

An automated dispersive liquid-liquid microextraction method of detecting and quanta N-nitrosamines in an aqueous sample. The method includes (a) extracting an aqueous solution containing the N-nitrosamines by mixing an extraction solvent and a dispersive solvent with the aqueous solution, such that the N-nitrosamines, or a portion thereof, re-distribute from the aqueous solution to the extraction solvent, (b) permitting the resulting mixture in (a) to form a two-phase mixture containing an aqueous phase comprising containing the aqueous solution with reduced amounts of the N-nitrosamines and an organic phase containing the extraction solvent with the N-nitrosamines extracted from the aqueous solution, (c) injecting the organic phase, or a portion thereof, into an injection port of a gas chromatograph coupled with at least one mass spectrometer, and (d) analyzing the N-nitrosamines by gas chromatography and mass spectrometry to detect and quantify the concentration of the N-nitrosamines in the aqueous solution.

A gas phase component analysis device and a gas phase component analysis method that can prevent degradation of the device due to an unnecessary component and can obtain excellent detection sensitivity are provided.

A gas phase component analysis device (1) includes a heating unit (2) configured to heat a specimen to generate a gas phase component composite, a first column (31) into which the gas phase component composite is introduced, a second column (32) that is a separation column connected with the first column (31) through a connection unit (33), an isothermal oven (3) housing the first column (31), the second column (32), and the connection unit (33), a detection unit (4) configured to detect a gas phase component having passed through the second column (32), and a suction unit (5) connected with the connection unit (33).

The present invention relates to a sample collection device for collecting volatile organic compounds from the air, as well as to a method for characterizing the volatile organic compounds and to the use of this device for characterizing the odorant signature of an individual and allowing a dog to identify an individual from his/her odorant signature.

Methods for identifying carbon derived from natural sources in a confectionary product are presented. Methods include separating, extracting and carbon dating components of a confectionary product, e.g., chewing gum or chewing gum base.

A system for preparing a test sample includes a vial holder, a needle trap connected to the vial holder, and a sample preparation station. The vial holder includes a vial chamber configured to hold a vial, a purge gas needle, and a needle trap heater. The needle trap includes a needle with the needle trap heater surrounding a distal end portion of the needle. A packing bed is disposed in the needle at the distal end portion. The sample preparation station includes a housing and a vial heater assembly including a vial heater and defining a cavity. The vial holder is configured to be received in the cavity in an installed position with the vial heater surrounding at least a portion of the vial.

The present invention relates to method for performing extraction of samples with low entrainment of air. More specifically, the invention relates to a microelution column (1) comprising a column body (2) including a bottom frit (3) and a top frit (4), wherein the column body includes an adsorbent (5) arranged between the bottom frit (3) and the top frit (4), and an optional filter frit (6) has been arranged above the top frit. The invention also relates to a plate including such columns, and a method of use.

A system includes an extraction system having an inlet channel and an outlet channel to receive fluid and to output processed fluid. The system also includes an extraction device to capture a first sample of fluid from the inlet channel, capture a second sample from the processed fluid from the outlet channel, and extract gas, liquid, or solids from the first sample and further gas, liquid, or solids from the second sample. Additionally, the system includes a gas, liquid, or solids analyzing device useable to analyze the gas, liquid, or solids to determine a first value of a chemical species of interest present in the first sample and to analyze the further gas, liquid, or solids to determine a second value of the chemical species of interest present in the second sample to determine an efficiency of the extraction system using the first value and the second value.

The present invention relates to a method for evaluating metabolic activity based on the concentrations of cortisol and its precursors and metabolites thereof in saliva collected by a swab for the measurement of adrenal steroids relating to biochemical stress evaluation and more particularly, to a method of comparing metabolic activity of physiologically different adrenal steroids by analyzing adrenal steroids including cortisol and cortisone extracted from a trace amount of saliva by mass spectrometry and evaluating the metabolic ratio between the two compounds as well as the absolute amount of each compound. Accordingly, the present invention can be utilized as an index for evaluating response to biochemical stress and related diseases by measuring the amounts of adrenal steroids including cortisol and cortisone in a trace amount of saliva collected with a swab.