The disclosure discloses a method for quickly and accurately analyzing polyphenol content in rapeseed oil, and belongs to the field of analysis of natural compounds. The separation method of the disclosure uses acetonitrile-water as an extractant to extract polyphenols from the rapeseed oil, and cooperates with a C.sub.18 adsorbent for purification, and then performs separation and purification. Compared with the traditional liquid-liquid extraction and solid-phase extraction, the method has an average recovery rate of polyphenols in the rapeseed oil of 81.31% to 102.95%, and RSDs of 0.86% to 8.03%, and has higher accuracy and precision. The method of the disclosure not only uses less organic reagents and causes less environmental pollution, but also reduces matrix interference and improves purification efficiency through optimization of the adsorbent. The method of the disclosure not only is simple to operate and low in cost, but also has less matrix interference and accurate results, and is suitable for the qualitative and quantitative determination of polyphenols in the rapeseed oil.

A system includes a phase behavior analysis unit having a housing, a heating system connected to the housing and arranged to heat an interior of the housing, a pressure cell positioned in the interior of the housing, and a three-way valve with one inlet fluidly connected to a chamber in the pressure cell and two outlets. The system also includes a gas chromatograph that is fluidly coupled to the chamber in the pressure cell via the three-way valve.

The present invention relates to a method for analyzing a metalloprotein in a biological sample capable of continuously maintaining conditions of LC-ICPMS constant to measure a metalloprotein with high data reliability. The method for analyzing a metalloprotein which is a complex in a biological sample, the metalloprotein being a complex in which a biomolecule and a metal element bind to each other, includes: treating a biological sample which has been subjected to a pretreatment by liquid chromatography to separate the metalloprotein, and analyzing the separated metalloprotein by inductively coupled plasma mass spectrometry, wherein an ammonium acetate solution is used as a mobile phase.

A system and method for determining an efficiency of gas extraction. A chamber allows inflow and outflow of the drilling fluid. An amount of gas extracted from a drilling fluid flowing through the chamber at a constant rate during a dynamic process is measured. A dissolution curve is obtained indicative of a gas remaining in the chamber after the dynamic process. An amount drawn from the chamber during a static process subsequent to the dynamic process is measured. An amount of gas from the drilling fluid during the static process is determined from a difference between the amount of gas drawn from the chamber during the static process and an amount of gas indicated by the dissolution curve. The gas extraction efficiency is determined from a ratio of the amount of gas extracted during the static process and the amount of gas extracted during the dynamic process.

The present invention refers to a method of purifying a glucagon-like peptide 1 analogs, the method comprising a two dimensional reversed phase high performance liquid chromatography protocol, wherein the first step is carried out at a pH value between 7.0 to 7.8 using a mobile phase comprising a phosphate buffer and acetonitrile, and the second step is carried out at a pH value below 3.0 using a mobile phase comprising trifluoroacetic acid and acetonitrile.

A device for preparing a liquid sample for a direct injection of a corresponding gaseous sample to a micro-gas chromatograph includes: a fluid space and a gas space, which spaces are separated by a semipermeable separating layer, the fluid space including a supply line for the liquid sample, and the gas space having an outlet connectable with the gas chromatograph. The fluid space and/or the gas space is associated with at least one heating element. The device absorbs a sample volume of approximately 10 l to 30 l. The separating layer has a thickness of 10 l to 300 l and pores having a size between 0.05 l and 5 l.

A microfluidic device for analysing a specimen comprises a loading area for loading the specimen of interest and an analytical column. The loading area is connected on two sides to a first duct and a second duct respectively, both integrated in the microfluidic device. The microfluidic device comprises a first integrated input connected to the first duct to take the specimen into the loading area, a first integrated output connected to the second duct to discharge the rest of the specimen, once it has flown through the loading area, and a second integrated output downstream the analytical column. The first integrated output is arranged for during a first loading period of time being in circuit connected to the first integrated input so as to load the sample into the loading zone of the device while preventing loss of specimen during loading of the sample into the analytical column.

Disclosed is a proteomic reactor, comprising a pipette tip, an ion exchange resin filler and a solid-phase extraction membrane. The solid-phase extraction membrane is filled into the lower end of the pipette tip, and the ion exchange resin filler is filled into the lower end of the pipette tip and is located above the solid-phase extraction membrane. The ion exchange resin filler is a strong cation exchange resin filler or a strong anion exchange resin filler. Disclosed is a protein chromatographic separation platform comprising the proteomic reactor and a liquid chromatography-mass spectrometer. Disclosed is the use of the proteomic reactor and protein chromatographic separation platform in the protein identification and protein quantitative analysis of a cell, a tissue or a blood sample.

A method for extracting pharmacogenetic DNA from dried blood that is retained in a solid resin collection device includes one or more of the steps of combining the dried blood and at least a portion of the solid resin collection device in a first vessel, sonicating the first vessel containing the dried blood and at least a portion of the solid resin collection device, moving at least some of the contents of the first vessel including the portion of the solid resin collection device to a solid resin spin column, adding an elution buffer to the solid resin spin column, centrifuging the solid resin spin column so that at least some of the contents of the solid resin spin column are transferred to a second vessel, and processing the contents of the second vessel by conducting one of capillary electrophoresis, Next-Generation Sequencing, DNA sequencing or genotyping, and mass spectrometry-based sequencing on the contents of the second vessel.

A method of analyzing a multi-component polymer comprising: (a) dissolving an multi-component polymer having a primary monomer and primary comonomer to form a first volume (soluble portion of multi-component polymer); (b) injecting a portion of the first volume into a chromatographic column to get elution first slices, leaving a second volume behind; (c) filtering the second volume to isolate multi-component polymer solids; (d) dissolving solids to form solution third solution (insoluble portion of multi-component polymer); (e) injecting a portion of third solution into the chromatographic column to get elution second slices; (f) obtain infra-red spectra at wavelengths suitable for the primary monomer and the primary comonomer of first and second elution slices, separately; and (g) for each elution slice, separately calculate: (i) the different polymer components (soluble and insoluble); and (ii) the comonomer content of each component (soluble and insoluble).