Disclosed is a proteomic reactor, comprising a pipette tip, an ion exchange resin filler and a solid-phase extraction membrane. The solid-phase extraction membrane is filled into the lower end of the pipette tip, and the ion exchange resin is filled into the lower end of the pipette tip and is located above the solid-phase extraction membrane. The ion exchange resin is a strong cation exchange resin or a strong anion exchange resin. Disclosed is a protein chromatographic separation platform comprising the proteomic reactor and a liquid chromatography-mass spectrometer. Disclosed is the use of the proteomic reactor and protein chromatographic separation platform in the protein identification and protein quantitative analysis of a cell, a tissue or a blood sample.

The present invention provides a method for determining glycosylation and terminal modifications of immunoglobulin during immunoglobulin purification process, which can simultaneously and rapidly determine glycosylation, N-terminal pyroglutamination and C-terminal de-lysination of immunoglobulin, including Step 1) separating immunoglobulin by using cation-exchange resin, and collecting different components in according to retention time; Step 2) denaturing the components of immunoglobulin obtained in step 1) with a denaturant, followed by reducing them with a reducing agent, to separate the light chain and heavy chain; Step 3) separating the light chain and heavy chain of immunoglobulin of step 2) by using reverse phase ultrahigh pressure liquid chromatography; Step 4) measuring the molecular weights of the light chain and heavy chain obtained in step 3) with mass spectrum; and Step 5) analyzing the chromatographic data obtained in step 3) and the mass spectrometric data obtained in step 4) to determine glycosylation and terminal modifications of the immunoglobulin.

A method for extracting an analyte in a sample is described. A sample and a solution in a microwave-extraction vial are microwave-heated and agitated. A vapor produced in the vial can be extracted into a liquid-phase medium contained in a porous membrane bag situated in the vial. The liquid-phase medium containing the vapor extract may then be analyzed for an analyte with gas chromatography-mass spectrometry.

Described herein are methods of assaying a biological sample, including adding a solvent to a composition of the biological sample to generate a mixture of an organic layer and an aqueous layer. The organic layer contains at least one lipid. The aqueous layer contains at least one metabolite. Also, described herein are methods of assaying a biological sample, including performing mass spectrometry on at least a portion of an organic layer to identify at least one lipid. Also, described herein are methods of assaying a biological sample, including performing mass spectrometry on at least a portion of an aqueous layer to identify at least one metabolite. Also, described herein are apparatus and kits for assaying a biological sample including at least one protein and at least one lipid or metabolite.

A monitoring method of dissolved greenhouse gases in wastewater includes: S1, collecting a wastewater sample, performing mud-water separation, and collecting a supernatant after the mud-water separation; S2, adding dilute sulfuric acid solution to the supernatant collected in a headspace vial to adjust pH of the supernatant to 1-4, and then tightening a cap of the headspace vial; S3, inverting the headspace vial, and checking whether there are air bubbles in the headspace vial; S4, injecting 5-10 mL of pure nitrogen into the headspace vial through a syringe, and discharging 5-10 mL of the wastewater sample through a conduit; S5, placing the headspace vial in a water bath constant temperature shaker, and shaking the headspace vial for 20-30 minutes; S6, extracting gases from an upper part of the headspace vial, and measuring concentrations of the gases; S7, quantitatively calculating concentrations of the dissolved greenhouse gases in the wastewater sample.

Provided is a method for quantifying a specific component contained in a measurement sample. The method includes: obtaining a measurement spectrum at each of a plurality of points in time by analyzing the measurement sample with chromatography; deriving an index value at each point of the plurality of points in time, by applying a filter for extracting the specific component, to the measurement spectrum at the point of the plurality of points in time; obtaining a chromatogram by arranging one or more index values at respective one or ones of the plurality of points in time; and quantifying the specific component based on a peak of the chromatogram.

Provided is a method for quantifying a specific component contained in a measurement sample. The method includes: obtaining a measurement spectrum at each of a plurality of points in time by analyzing the measurement sample with chromatography; deriving an index value at each point of the plurality of points in time, by applying a filter for extracting the specific component, to the measurement spectrum at the point of the plurality of points in time; obtaining a chromatogram by arranging one or more index values at respective one or ones of the plurality of points in time; and quantifying the specific component based on a peak of the chromatogram.

The present invention relates to a method for detecting and/or quantifying an analyte in a sample using mass spectrometry. The method of the invention comprises: extracting the analyte from the sample using solid phase extraction (SPE) so as to obtain an SPE extract comprising the analyte, concentrating the analyte, said concentrating comprising evaporating solvent from the SPE-extract; and detecting and/or quantifying the analyte in the sample using mass spectrometry.

The invention relates to a method and a system for analyzing one or more compounds in a hydrocarbon-containing sample. The method comprises steps of: a) injecting the sample and a first solvent into a chamber so that the one or more compounds in the sample form a precipitate in the chamber; b) passing the precipitate from the chamber to a filter device, wherein the precipitate is captured in the filter device; c) passing a second solvent through the filter device while bypassing the chamber, so as to dissolve the one or more compounds in the precipitate captured in the filter device; and d) detecting the one or more compounds downstream of the filter device. The system comprises a chamber, a sample feeding line, a first solvent feeding line, a filter device, a transfer line, a second solvent feeding line, and a detector.

A gas-liquid separator has a double cylinder structure consisting of an outer cylinder and an inner cylinder. An injection port that injects the fluid from the outside to the inside is provided on the inner wall at the upper end of the outer cylinder. An ejection port that ejects the liquid separated from the fluid is provided at the lower end of the outer cylinder. A lower end of the inner cylinder is open in the inside of the outer cylinder. The upper end of the inner cylinder penetrates through the closed upper end of the outer cylinder, and has an exhaust port that exhausts the gas separated from the fluid. A discharge port for discharging a solvent from the outside to the inside is provided, in addition to the injection port of the fluid, on the inner wall at the upper end of the outer cylinder.