Provided is a preparative separation liquid chromatograph system and preparative separation condition searching method which allows for an easy setting of the preparative separation condition. A sample temporally separated into components by a separation column is introduced into a detector and a fraction collector, with each component fractionated and collected by the fraction collector based on the result of a detection by the detector. A controlling and processing unit holds the following data for each sample or compound in the form of a database: chromatogram data obtained when a liquid chromatograph analysis in a preparative separation condition searching mode is performed for various standard samples under a search condition; and chromatogram data obtained when a liquid chromatograph analysis in a preparative separation mode is performed under one or more sets of preparative separation conditions for the various standard samples, along with the preparative separation condition used in this analysis.

A method of sampling ingredients of an oil-bearing inclusion includes a) providing a first container and a second container, an external diameter of the first container being smaller than an internal diameter of the second container, and the first and second containers both being transparent; b) adding a solvent into the first container and sealing said first container; c) adding an oil-bearing inclusion sample into the second container, and putting the first container that contains the solvent and is sealed in step b) into the second container; and d) using a laser to ablate the oil-bearing inclusion sample contained in the second container that is sealed in step c), and using the laser to break an end portion of the first container close to the sample on condition that the second container is maintained complete, so as to allow the solvent in the first container to enter the second container.

The culture medium processing system includes a controller (100) configured to control operation of a sample dispensing part (20), a reagent dispensing part (26) and a transport arm (24) to deproteinize a sample, wherein the controller (100) is configured to dispense a methanol solution into an empty filtration container (50) to perform a conditioning of a filtration filter (52) disposed in the filtration container (50), then dispense a sample into the filtration container (50), add an acetonitrile solution as a deproteinization agent to the sample in the filtration container (50), and then perform a filtration process in the filtration part (30).

The culture medium processing system includes a controller (100) configured to control operation of a sample dispensing part (20), a reagent dispensing part (26) and a transport arm (24) to deproteinize a sample, wherein the controller (100) is configured to dispense a methanol solution into an empty filtration container (50) to perform a conditioning of a filtration filter (52) disposed in the filtration container (50), then dispense a sample into the filtration container (50), add an acetonitrile solution as a deproteinization agent to the sample in the filtration container (50), and then perform a filtration process in the filtration part (30).

The present application relates to methods for determining the concentration of one or more hormones in a sample by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

The present application relates to methods for determining the concentration of one or more hormones in a sample by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Described is a method for vacuum degassing of a liquid such as a solvent for a liquid chromatography system. The method includes modulating application of a vacuum to a fluid channel of a degasser so that each volume of a liquid drawn from the degasser experiences a residence time that is equal to the residence times of the other volumes. The residence time is determined as a time that the volume resides in the fluid channel under application of the vacuum and to a magnitude of the applied vacuum. The method is advantageous for use with liquid chromatography systems where differences in the diffusion rates of solvents into the degasser vacuum can otherwise introduce error into the composition gradient of a mobile phase.

Described is a method for vacuum degassing of a liquid such as a solvent for a liquid chromatography system. The method includes modulating application of a vacuum to a fluid channel of a degasser so that each volume of a liquid drawn from the degasser experiences a residence time that is equal to the residence times of the other volumes. The residence time is determined as a time that the volume resides in the fluid channel under application of the vacuum and to a magnitude of the applied vacuum. The method is advantageous for use with liquid chromatography systems where differences in the diffusion rates of solvents into the degasser vacuum can otherwise introduce error into the composition gradient of a mobile phase.

The disclosed system and method concentrates and enriches a chemical sample while removing water and/or CO2 prior to analysis, improving detection limits and repeatability of quantitative chemical analysis without the need for cryogenic or sub-ambient cooling. The system can include a valve system, a dewpoint control zone, and a multi-capillary column trapping system (MCCTS). During a first time period, the valve system can couple the dewpoint control zone to the MCCTS. During a second time period, the valve system can couple the MCCTS to the chemical separation column such the dewpoint control zone is bypassed. Excess water included in the sample can condense in the dewpoint control zone as the sample transfers to the dewpoint control zone and MCCTS. When the sample is transferred from the MCCTS to the chemical separation column, the condensed water in the dewpoint control zone is not transferred to a chemical separation column.

The disclosed system and method concentrates and enriches a chemical sample while removing water and/or CO2 prior to analysis, improving detection limits and repeatability of quantitative chemical analysis without the need for cryogenic or sub-ambient cooling. The system can include a valve system, a dewpoint control zone, and a multi-capillary column trapping system (MCCTS). During a first time period, the valve system can couple the dewpoint control zone to the MCCTS. During a second time period, the valve system can couple the MCCTS to the chemical separation column such the dewpoint control zone is bypassed. Excess water included in the sample can condense in the dewpoint control zone as the sample transfers to the dewpoint control zone and MCCTS. When the sample is transferred from the MCCTS to the chemical separation column, the condensed water in the dewpoint control zone is not transferred to a chemical separation column.