A chromatographic device includes a primary channel having a cross-sectional area and characteristic length such that analyte travel within the primary channel is substantially convective. A plurality of secondary channels each having a cross-sectional area and characteristic length such that analyte flow into and out of a secondary channel is substantially diffusive, each of the plurality of secondary channels having an entrance in fluidic communication with the primary channel wherein the entrance intersects the primary channel.

The present disclosure is directed at the selection and design of columns for liquid chromatography including liquid chromatography devices and systems and corresponding methods of operation, particularly in the field of high pressure liquid chromatography (HPLC).

The present invention provides a gas chromatography system (GC) including a GC column configured for a chromatographic separation of a sample comprising one or more analytes, a GC detector connected to the exit of the GC column, and a controller connected to the GC system. The controller is configured to generate a simulated chromatographic separation using a chromatographic model that calculates at least one chromatographic parameter of the analyzed sample. The controller further configured to execute a chromatographic separation of the sample and execute chromatographic performance monitoring that includes a comparison of at least one chromatographic parameter to the simulated chromatographic separation and/or reference chromatographic separation, determine if at least one chromatographic parameter has fallen outside of a performance control limit and/or predict if the chromatographic parameter will fall outside the performance control limit, and perform an automated GC troubleshooting procedure to determine the cause of the performance issue.

A column oven includes a substantially sealed space surrounded by wall surfaces made of a heat conductive material to accommodate an analytical column, a heater for heating the wall surfaces made of the heat conductive material, and a heat insulating material surrounding an outside of the wall surfaces made of the heat conductive material. That is, a space inside the column oven in which the analytical column is accommodated is formed as a substantially sealed structure surrounded by the heat conductive wall surfaces, and the wall surfaces surrounding the substantially sealed space are heated by the heater, thereby heating the substantially sealed space uniformly from circumferential directions.

This disclosure provides chromatography columns that are packed with incompressible media such as ceramic hydroxyapatite particles, which exhibit high separation performance that is robust to transportation and multiple uses. The columns can be made by applying axial compression using rigid bodies, such as porous frits and/or flow regulators.

The present invention is directed to a method for performing size exclusion chromatography. Embodiments of the present invention feature devices and methods for improving the speed and separations of size exclusion chromatography using a stationary phase material comprising small particles (<2 micron in diameter).

A system and method for determining concentration of a constituent of a sample fluid includes a flow cell with a light source emitting incident light to a proximal end thereof. Media disposed within the flow cell supports nanostructures that are substantially transparent in at least a portion of the incident light spectrum. The nanostructures adsorb or absorb the constituent to attain a concentration that is a multiple of the concentration of the constituent in the sample fluid. A sensor detects transmitted light exiting from the media, and generates outputs corresponding to a spectrum of the transmitted light. A processor captures the sensor outputs and compares the incident light spectrum to the transmitted light spectrum to generate an absorbance spectrum. The absorbance spectrum is used to calculate the concentration in the nanostructures, which is then used with the predetermined multiple to calculate the sample concentration.

A chromatographic device includes a primary channel having a cross-sectional area and characteristic length such that analyte travel within the primary channel is substantially convective. A plurality of secondary channels each having a cross-sectional area and characteristic length such that analyte flow into and out of a secondary channel is substantially diffusive, each of the plurality of secondary channels having an entrance in fluidic communication with the primary channel wherein the entrance intersects the primary channel.

The present disclosure relates to a chromatographic stationary phase having a uniform polymer density, and related methods. In particular, the present disclosure relates to a method of forming a uniformly dense stationary phase inside a chromatography column.

A repeatable method for detecting circulating tumor cells in vitro is provided. The method involves combining a test sample from a patient suspected of having circulating tumor cells, and a non-lytic adenoviral system, and culture media for the cells. The adenoviral system utilizes (i) a first replication-defective adenoviral particle in which an expression cassette is packaged, said expression cassette comprising an adenoviral 5 and 3 ITRs and a tumor-specific promoter; and (ii) a coding sequence for a reporter protein which is expressed in the presence of circulating tumor cells, and an adenoviral 3 ITR. The test sample and the non-lytic adenoviral system are incubated for a sufficient time to permit expression of the reporter protein, and measuring reporter protein expression in the test samples, whereby presence of reporter expression indicates the presence of circulating tumor cells in the sample. Because the system is non-lytic, the testing can be repeated on the cells which remain viable in culture. Also provided is a method for enriching test samples having circulating tumor cells and a microfluidics device suitable for CTC-specification identification and enumeration.