A chromatography column frame comprising at least two legs, a support arrangement connected to said at least two legs, and a holder connected to said support arrangement. Said holder is arranged to releasably hold an adaptor rod of a chromatography column, so that the adaptor rod is prevented to move in a horizontal direction and so the adaptor rod is allowed to move in a vertical direction.

A chromatography column assembly includes a standard column length of at least 100 mm and a plurality of columns connected in series. Each of the plurality of columns add up in length to the standard column length. Further disclosed are liquid chromatography systems including the described chromatography assemblies, and chromatographic methods deploying the described chromatographic assemblies.

Exemplary embodiments integrate a tee or valve into an outlet end fitting of a liquid chromatography column. The tee or valve is suitable for providing additional fluidic flow paths to ports of the end fitting and eliminates the need for post-column fluidic conduits connecting to tees or valves to insert fluidic inputs or divert flow to outputs. This integration decreases the distance that eluent from the liquid chromatography column has to travel to reach a detector relative to systems that use external tees or valves while providing tee/valve functionality and reducing the fluidic volume post-column. As a result, the exemplary embodiments help decrease sample dispersion.

To analytically determine concentration of dissolved gases in a water pipeline, a water sample is drawn from a source carrying water with dissolved gas, through a water source port of a four-way valve. The water sample is flowed from the water source port towards a syringe port of the valve and into a syringe fluidically coupled to the syringe port to hold the water sample. Inert gas is drawn through an inert gas port of the valve from an inert gas source and is flowed from the inert gas port towards the syringe port and into the syringe. A mixture of the water sample and the inert gas is flowed from the syringe port towards an analyzer port of the valve and into an analyzer fluidically coupled to the analyzer port.

The present invention relates to a nozzle for an air trapping device configured to remove air from a fluid, the nozzle comprising a body having an input opening configured to receive the fluid and an output opening configured to distribute the fluid along an edge of the output opening, wherein the edge comprises a control element configured to reduce surface tension of the fluid. The present invention further relates to an air trapping device configured to remove air from a fluid and comprising the nozzle.

The present invention describes a device for supplying or collecting from a column forming part of an assembly of columns in series, for use in a simulated moving bed separation process. The present device can be used to very substantially reduce the non-selective volumes in each column.

In some examples, a load adapter may include a body including an elongated shaft that includes an inlet passage that is in fluid communication with at least one outlet hole. The at least one outlet hole may be dimensioned to provide an opening area that is between approximately 5% to approximately 90% of a face of the body.

The field of chromatography, specifically the field of expanded bed chromatography. The invention is an expanded bed chromatography apparatus 1 which has an elongate tube 4 defining an operating volume 8 and having a central axis 7. The top end of the tube is sealed with a cap 2 having an exit port 3. The other end of the tube 4 is connected to a base 5. The base includes a process fluid inlet having a plurality of outlet apertures 10a, 10b, 10c in fluid communication with the operating volume of the tube and an inlet aperture 11 in fluid communication with the plurality of outlet apertures. Each of the plurality of outlet apertures is spaced radially from the central axis of the tube. In use, a mobile phase of process fluid passes up through a particulate media in the tube from the inlet to the exit port in a cap at the top of the apparatus. Target product is adsorbed onto the particulate media and is subsequently eluted from it. Alternatively, impurities are adsorbed on the particulate media and the target solution passes through the expanded bed for collection.

A flow cell for a liquid chromatography detector comprises a substrate formed of a glass material; a fluidic channel extending through the substrate; and at least one gas filled region formed in the substrate along at least a portion of a length of the fluidic channel. A portion of the glass material separates the fluidic channel and the gas filled region. An interface between the gas filled region and the portion of the glass material separating the fluidic channel and the gas filled region enables total internal reflection of light propagating along the fluidic channel.