A sorbent tube apparatus (10) for high-pressure fluid sample analysis, the sorbent tube apparatus (10) comprising a pressurisable housing (32) having first and second fluid ports (48a, 48b) and defining a fluid chamber (34) therein; and a sorbent tube (12) mountable within the pressurisable housing (32), the sorbent tube (12) extending from one of the first and second fluid ports (48a) and spaced apart from the other of the first and second fluid ports (48b) to be in fluid communication with the fluid chamber (34), thereby enabling in use pressure equalisation between the sorbent tube (12) and fluid chamber (34). A method of analysing high-pressure fluid, an analytic probe apparatus, a further sorbent tube apparatus and method of preventing or limiting damage to a sorbent tube during high-pressure fluid sampling are also provided.

Provided herein in some embodiments is a column including a housing assembly and a cartridge assembly. The housing assembly can include a housing top, a housing bottom, and a housing siding. The housing siding can be fixedly coupled to the housing top and the housing bottom forming hermetic seals therebetween. The cartridge assembly can include a cartridge top, an outer frit, and an inner frit disposed within the outer frit. A toroidal space can defined by the cartridge top, the outer frit, the inner frit, and the housing bottom. The toroidal space can be configured to hold a stationary phase for radial flow column chromatography. Also provided herein in some embodiments is a process including assembling the cartridge assembly, assembling the housing assembly about the cartridge assembly, and pressure testing the column. In some embodiments, the process can further include charging the toroidal space with a stationary phase.

A liquid chromatography (LC) column includes a wall having a length along a central axis from the inlet end to the outlet end, the wall enclosing a column interior and having a column radius relative to the central axis, the wall comprising a structured portion configured such that the column radius varies along the length; and a plurality of particles packed in the column interior, wherein at least some of the particles are in contact with the structured portion.

A chromatography column has a column assembly that further includes a column jacket tube and a column liner tube. The column assembly is configured with end fittings that have compression limiting features and are able to withstand very high pressure, such as used in UHPLC, in conjunction with flange portions of the column liner tube that seal the column assembly. The column liner tube may be biocompatible and may have a smooth consistent inner diameter of less than 2 mm, and an inner diameter of 1 mm in particular embodiments. The end fittings for the microtube assembly may ensure durability and reliability by preventing over- or under-tightening.

The invention discloses a piston for a process column, said piston comprising a top side and a bed contact side, wherein said top side comprises a drainage trench.

The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

A technique for replacing a separation column includes: a driving device supporting a first pipe along a first direction; a fixing member that fixes a second pipe to be opposed to the first pipe along the first direction; a column case between the first pipe and the second pipe, having a first and second holes communicating with the first and second pipes, respectively; and an interlocking mechanism that moves the column case in the first direction in conjunction with movement of the first pipe. The column case allows a column cartridge retaining a separation column to be connected and disconnected. The driving device moves the first pipe and the interlocking mechanism such that two states are reversibly taken; a closed state in which the separation column is between the first and second pipes and an open state in which the first and second pipes are positioned outside the column case.

To analytically determine concentration of dissolved gases in a water pipeline, a water sample is drawn from a source carrying water with dissolved gas, through a water source port of a four-way valve. The water sample is flowed from the water source port towards a syringe port of the valve and into a syringe fluidically coupled to the syringe port to hold the water sample. Inert gas is drawn through an inert gas port of the valve from an inert gas source and is flowed from the inert gas port towards the syringe port and into the syringe. A mixture of the water sample and the inert gas is flowed from the syringe port towards an analyzer port of the valve and into an analyzer fluidically coupled to the analyzer port.

A column filling apparatus mainly includes a tank, a stirrer being an agitator, a pump, a resin bottle, first to eleventh pipes, first to fourth valves composed of three-way valves, and fifth and sixth valves composed of two-way valves. A suction port is mounted on a leading end of the fifth pipe, and the suction port is inserted into an inner part from a top opening of the resin bottle. The other end of the fifth pipe is connected to the first valve. The first valve is connected to the first pipe, the second pipe, and the fifth pipe, and connects or disconnects these pipes to or from each other. An end of the first pipe is mounted near a bottom of the tank, and is opened near an inner bottom surface of the tank.

The present disclosure relates to methodologies, systems, apparatus, and kits for introducing a sample within a chromatography system. A makeup pump is configured to pump a makeup fluid through a first restrictor into the chromatography system upstream of the column and downstream of a mobile phase pump. The first restrictor is located upstream of a column and downstream of makeup pump and a sample fluid pump. Decreasing an output volume of the makeup pump can direct a sample fluid from the sample fluid pump through the first restrictor to the column. Increasing an output volume of the makeup pump can direct the sample fluid to a second restrictor located downstream of the makeup pump and in parallel with the column and the detector.