The invention relates to a flow cell for absorption detection, in which a tube through which flow is to pass is held at its opposite ends in a supporting flange in each case and is suspended in a substantially cantilevered manner, the two supporting flanges being connected rigidly to each other in order to avoid stresses accidentally introduced into the tube.

Methods, systems, devices, and products for evaluating a fluid. Methods include introducing a sample comprising the fluid to a solvating fluid at a point in a chamber associated with the instrument at a first time to create a heterogeneous admixture; measuring concentrations of each of a plurality of components in the admixture at a plurality of distances from the point in the chamber at, at least one additional time later than the first time, each of the plurality of distances being non-zero; and estimating a relative concentration for each of the plurality of components in the fluid by extrapolating the relative concentration of each of the plurality of components in the sample at the point at the first time using the measured concentrations in the admixture at the plurality of distances.

The present invention relates to method of using spectroscopy for real time measuring of concentration of desired product and using measured data for monitoring and control of chromatography. It develops a method and system for measuring real-time concentration of clarified harvest and that of flow through of loading step of the chromatography and using measured data for determining breakthrough in real-time. The two modes of operation are used viz. first mode (Part A) uses a single near infrared spectroscopy (NIR) flow cell prior to the continuous chromatography column to ensure optimal loading in each cycle based on dynamic binding capacity studies carried out previously with the desired Protein A resin and second mode (Part B) uses two near infrared spectroscopy (NIR) flow cells, one before and one after the column, to detect the breakthrough curve (from 1% breakthrough onwards).

An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

A flow cell for a detector in a liquid chromatography system includes a flow cell body, an inlet, an outlet, at least two windows situated on opposing sides of the flow cell body, which are transparent for light, and at least two electrodes configured for a conductivity measurement. A detection channel is formed within said flow cell body, fluidly connecting said inlet and outlet, includes an optical path situated in between said two windows such that a light absorption measurement may be performed for a liquid passing through the detection channel, and a conductivity path formed by at least partially drilling through said electrodes to allow for a physical contact of a liquid passing through the detection channel. A detector or liquid chromatography system including such a flow cell as well as uses of the flow cell, detector or liquid chromatography system are also described.

A detector for liquid chromatograph includes a light source, a flow cell that includes a linear capillary, a holding member that holds one end portion of the capillary, an entrance port for allowing light to enter one end of the capillary, and an emission port for allowing light to be emitted from another end of the capillary, a condensing mirror for guiding light from the light source to the entrance port, and a light receiver for detecting the light emitted from the emission port, a lens is provided between the one end of the capillary and the condensing mirror, and the lens is arranged so as to parallelize light in a center region around an optical axis among light directed to the one end of the capillary from the condensing mirror and distance a first reflection position of the light in the center region from the one end.

A flow cell and a liquid chromatographic unit are provided. The flow cell includes a housing, a cell core, a liquid-core waveguide, an inlet connection assembly and an outlet connection assembly. The cell core is provided in the housing, and is provided with a liquid feed recess, a liquid channel and a liquid discharge recess therein. The liquid-core waveguide is provided in the liquid channel. The inlet connection assembly is provided at an end of the cell core, and includes an inlet press block, a liquid feed tube, and a light entering tube. The outlet connection assembly is arranged at another end of the cell core and is provided with a light exit hole.

A method to simulate distillation of a petroleum stream by gas chromatography can include separating the petroleum stream with a gas chromatograph as a function of boiling point; passing the separated petroleum stream through a vacuum ultraviolet detector to yield data comprising a vacuum ultraviolet signal as a function of boiling point; integrating the vacuum ultraviolet signal as a function of boiling point over two or more wavelength ranges to derive relative concentrations of two or more components of the separated petroleum stream that correspond to the two or more wavelength ranges.

An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.