A flow cell for a detector in a liquid chromatography system includes a flow cell body, an inlet, an outlet, at least two windows situated on opposing sides of the flow cell body, which are transparent for light, and at least two electrodes configured for a conductivity measurement. A detection channel is formed within said flow cell body, fluidly connecting said inlet and outlet, includes an optical path situated in between said two windows such that a light absorption measurement may be performed for a liquid passing through the detection channel, and a conductivity path formed by at least partially drilling through said electrodes to allow for a physical contact of a liquid passing through the detection channel. A detector or liquid chromatography system including such a flow cell as well as uses of the flow cell, detector or liquid chromatography system are also described.

A flow cell includes a housing, a window member, and a pressing member. The housing includes a cell channel in which a sample flows through, has, on at least one end side of the cell channel, an opening communicating with the cell channel, and has a flat surface at an edge of the opening. The window member has a lens portion at a central portion and a peripheral edge portion whose one surface and the other surface are flat. In the window member, one surface of the peripheral edge portion is provided facing the flat surface of the housing so as to seal the opening.

A flow path plate for analysis of a component in a liquid sample, flowing within the flow path plate, by irradiating the liquid sample with measurement light is provided. The flow path plate includes a plate body having transparency; a flow path formed within the plate body and through which the liquid sample passes; a chamber provided in a part of the flow path and formed by a space having a cross-sectional area greater than cross-sectional areas of other parts of the flow path; and an internal part for detection disposed in the chamber and having a through hole. The through hole has an inner wall surface and constitutes a part of the flow path. The measurement light passes through the through hole. The internal part is configured to inhibit transmission of the measurement light from the inner wall surface of the through hole to the outside.

A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.

Techniques and apparatus for thermally controlled interfaces for separation devices and optical flow cell devices with minimized post-column volumes are described. In one embodiment, for example, a column-optical cell assembly may include an insulating device, a chromatography column arranged within the insulating device, and an optical flow cell in fluid communication with the chromatography column, the chromatography column arranged within a minimum distance of the optical flow cell.

A flow cell includes a housing, a window member, and a pressing member. The housing includes a cell channel in which a sample flows through, has, on at least one end side of the cell channel, an opening communicating with the cell channel, and has a flat surface at an edge of the opening. The window member has a lens portion at a central portion and a peripheral edge portion whose one surface and the other surface are flat. In the window member, one surface of the peripheral edge portion is provided facing the flat surface of the housing so as to seal the opening.

Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.

A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.

Methods, systems, devices, and products for evaluating a fluid. Methods include introducing a sample comprising the fluid to a solvating fluid at a point in a chamber associated with the instrument at a first time to create a heterogeneous admixture; measuring concentrations of each of a plurality of components in the admixture at a plurality of distances from the point in the chamber at, at least one additional time later than the first time, each of the plurality of distances being non-zero; and estimating a relative concentration for each of the plurality of components in the fluid by extrapolating the relative concentration of each of the plurality of components in the sample at the point at the first time using the measured concentrations in the admixture at the plurality of distances.

Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.