The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

A near-infrared (NIR) quality monitoring method used in column chromatography for extracting conjugated estrogens (CEs) from pregnant mare urine (PMU), that includes steps of: collecting an eluate obtained from column chromatography of a PMU stock solution as a to-be-tested sample; subjecting the to-be-tested sample to near-infrared spectroscopy (NIRS) to obtain raw spectral data, eliminating abnormal spectral values from the raw spectral data by a Mahalanobis distance method based on L1-PCA, and importing spectral data obtained after the abnormal spectral values are eliminated into a correction model to obtain a CE content in the to-be-tested sample; the correction model is a linear equation illustrating a relationship between true values and measured values, and the measured values refer to the NIR spectral data obtained after the abnormal spectral values are eliminated; and the CEs comprise one or more of sodium 17α-dihydroequilin sulfate, sodium equilin sulfate, and sodium estrone sulfate.

A method for rapid differential diagnosis of infection using supercritical fluid chromatographic separation of quorum sensing molecules as biomarkers for infection agents.

A method for rapid differential diagnosis of infection using supercritical fluid chromatographic separation of quorum sensing molecules as biomarkers for infection agents.

A method for quantifying Hex4, lyso-GM1, Fuc-GlcNAc-Asn, or lyso-sulfatide included in cerebrospinal fluid, the method including adding an internal standard substance to a solution including the cerebrospinal fluid, submitting the solution including the cerebrospinal fluid, to which the internal standard substance has been added, to liquid chromatography to obtain an eluate, and subjecting the eluate to mass analysis.

The disclosure provides methods, kits and systems for detecting a metabolite that is metabolized from a nucleotide in reverse transcriptase inhibitor in a biological sample obtained from a subject, and uses thereof in monitoring adherence to pre-exposure prophylaxis and counseling subjects who are engaged in or prescribed pre-exposure prophylaxis. The present disclosure also provides methods of preventing HIV infection in patients at risk of contracting infection by monitoring adherence to a regimen and adjusting or modifying the dosing schedule of the regimen accordingly. The metabolite may be detected using proteomic methods, including but not limited to antibody based methods, such as a lateral flow immunoassay or lab based assays such as semi-quantitative LC-MS/MS.

Presented are improved and more sensitive methods for measuring levels of phytoestrogens in a biological sample, and specifically in a human urine sample, employing a High Pressure Liquid Chromatography method, coupled with a photodiode array analysis detection system. Calibration curve preparation for each of a panel of phytoestrogen analytes, including daidzein, equol and genistein, are provided employing techniques that demonstrate greater accuracy and sensitivity of sample level measurement. Clinically applicable techniques suitable for large population scale screening, diet and gut microflora characterization and disease analysis and correlation in human populations, such as in at risk breast cancer populations, through monitoring of phytoestrogen levels, is disclosed.

The present invention relates to a method of purifying whole HCV particles, the method comprising the steps of a) providing a cell culture supernatant comprising virus particles, b) purification and/or concentration of the cell culture supernatant, c) purification and/or concentration of the product of above step b) using steric exclusion chromatography (SXC) at alkaline pH in the range of 8-10, d) purification and/or concentration of the product of above step c) using sulphated cellulose membrane absorbers (SCMA), e) obtaining purified whole virus particles.

Provided are two-dimensional chromatography systems and methods for separating and/or analyzing complex mixtures of organic compounds. In particularly, a two-dimensional reversed-phase liquid chromatography (RPLC)—supercritical fluid chromatography (SFC) system is described including a trapping column at the interface which collects the analytes eluted from the first dimension chromatography while letting the RPLC mobile phase pass through. The peaks of interest from the RPLC dimension column are effectively focused as sharp concentration pulses on the trapping column, which is subsequently injected onto the second dimension SFC column. The system can be used for simultaneous achiral and chiral analysis of pharmaceutical compounds. The first dimension RPLC separation provides the achiral purity result, and the second dimension SFC separation provides the chiral purity result (enantiomeric excess).

A device includes camera hardware configured to capture image data associated with an output signal area of a biological chromatographic test strip, and processing circuitry configured to determine, based on the image data, respective intensities of a control line displayed in the output signal area of the biological chromatographic test strip, a hook line displayed in the output signal area of the biological chromatographic test strip, and a test line displayed in the output signal area of the biological chromatographic test strip. The processing circuitry is configured to determine a relative intensity between the test line and at least one of the hook line or control line using the respective intensities, and to detect, based on the relative intensity, a presence of a target analyte in a test sample submitted via the biological chromatographic test strip.