Systems, apparatuses, kits, and methods for purification and analysis of analytes having a broad range of hydrophobicities by liquid chromatography-mass spectrometry (LC-MS). Using one set of liquid chromatography columns, one set of mobile phase buffers, and, optionally, a single ionization method (e.g., electrospray ionization), a wide range of analytes can be purified and analyzed on a liquid chromatography-mass spectrometry (LC-MS) system. LC-MS purification and analysis of analytes having a broad range of partition coefficients is accomplished by selecting LC run parameters and MS system parameters that are particular to different classes of analytes without having to make column or buffer changes or any other hardware configuration changes to the LC-MS system. The methods, systems, and kits described herein provide for substantially increased speed/throughput and ease of use for a wide range analytes with essentially no compromise in specificity for individual analytes relative to previously described methods.

A debittering Kudingcha extract, a preparation method and a fingerprint detection method thereof are disclosed, which relates to the technical field of extract preparation and detection. The extract is prepared from Kudingcha by enzymolysis, water extraction, concentration, drying, purification and enrichment by a macroporous resin column, and drying. When the macroporous resin column is arranged for purification and enrichment, it is first rinsed with pure water, and then eluted with an ethanol solution with volume fraction of 30-80%, and an ethanol eluent is collected. The Kudingcha extract is purified with macroporous resin, which can retain its effective ingredients to the greatest extent while reducing the bitter taste, and enhance the effect of improving energy and resisting fatigue. The quality control of products through fingerprint is conducive to the improvement of product stability and further promotion of products.

A lacrimal tear flow measurement device, and methods of manufacture and use, are described that includes a polymer microcapillary tube or similar structure having at least one end coated on the outside with soft silicone rubber and one end treated on the inside to be hydrophobic. The hydrophobic end keeps liquid from escaping or entering that end while allowing air to pass. The rest of the tube's insides may be hydrophilic or a neutral hydrophobe. As a Schirmer's test strip replacement, the entrance end of the device can be touched to the lacrimal lake of a patient's eye to collect suck up, or merely collect, tear fluid within the collection tube for weighing, volume measurement, or other analysis. Long-term collection devices for wear between doctors' visits can have a bypass channel allowing liquid to flow back onto the eye.

A method of separating a liquid sample containing viral vectors includes flowing the liquid sample into an anion exchange column. A first viral vector contains a targeted genetic material and a second viral vector contains essentially no genetic material or a non-targeted genetic material, wherein the targeted genetic material is different than the non-targeted genetic material. A mobile phase can be flowed into the anion exchange column, wherein the mobile phase includes a buffer solution A and a buffer solution B. The buffer solutions A and B both include volatile buffer salts. The first viral vector and the second viral vector can be separated so that the first viral vector and the second viral vector elute off the anion exchange column at different times and then be detected.

The present invention relates to a method for metabolite sampling and analysis for reproducibly sampling as many metabolites as possible in a urine sample without changing to metabolites. The method has effects of presenting a biomarker detection method according to the sex or the like, by establishing optimal conditions for metabolite sampling in urine samples and presenting a metabolite comparison analysis method between different groups on the basis of the optimal conditions.

A method for analyzing aromatic compounds, and a reagent kit for LC-MS analysis of aromatic compounds. The method includes preparing a diazonium reagent, contacting aromatic compounds in a sample with the diazonium reagent to form an analyte; and measuring an amount or ratio of the analyte. The reagent kit includes a diazonium reagent, wherein the diazonium reagent includes (i) a diazonium salt that contains a diazonium ion; (ii) an amine and nitrous acid; and/or (iii) a nitrite and an acid.

Described herein are methods and systems for performing chemical or enzymatic reactions using electrophoresis. Devices, systems, and methods for preparing a library of tagged nucleic acid fragments from a target double-stranded nucleic acid using electrophoresis are also provided. Application of one or more electric fields causes molecules to migrate through the electrophoresis gel matrix.

Machines and methods are for performing fully-automated biological evaluation and chemical analysis. A pretreatment module is used in treatments of enriching, concentrating and purifying a sample to be analyzed. A component separation module is used for carrying out separation of multiple compounds in mother liquor to be analyzed. A monitoring and identifying module is for monitoring and collecting chromatographic signals of the separated liquid effluent in real time, as well as quantitative detection of suspicious compounds. A component collection module is used in operations of collecting, transferring and dissolving, redissolving, and pipetting the separated liquid effluent. A biological evaluation module is for cell culture and detection of cytotoxic effect and toxic targets. A data processing and automated control module is for acquisition, arrangement and analysis of the integrated data. The machines and methods provide an efficient, stable and normalized standard operation condition for the screening of toxicity of compounds.

An apparatus includes a mobile computing device (902) physically coupled to a lightbox (904). The apparatus includes camera hardware configured to capture image data associated with an output signal area of a biological chromatographic test strip (410) inserted into a receiving slot of the lightbox. The apparatus further includes processing circuitry in communication with the camera hardware, the processing circuitry being configured to determine, based on the image data captured by the camera hardware, a concentration of a target analyte in a test sample submitted via the biological chromatographic test strip. The apparatus further includes an interface in communication with the processing circuitry, the interface being configured to output data indicative of the concentration of the target analyte determined by the processing circuitry.

The present technology relates to a method of analyzing a sample including an analyte. The method includes injecting the sample including the analyte into a mobile phase. The mobile phase includes a metal chelator additive having a concentration between about 1 ppm to about 10 ppm. The method also includes separating the analyte using liquid chromatography and analyzing the analyte using a mass spectrometer, an ultra-violet detector, or a combination thereof.