Methods and system for chromatic confocal microscopy are described. One example chromatic confocal microscope system includes a hyperchromatic objective lens configured to focus the light of different wavelengths onto different corresponding focal planes that are separated from one another within a sample object, focusing optics positioned to receive multi-spectral light reflected from the sample object after passing through the hyperchromatic objective lens, a detection slit to receive light from the focusing optics and to block at least a portion of light that is incident thereon, and a grating positioned to receive light after passing through the detection slit and to produce spatially separated light of different wavelengths to enable the detection of spatially separated light by an imaging sensor. The described chromatic confocal microscopes may be used to develop low-cost chromatic confocal endoscopes for disease diagnosis of human internal organs in vivo.

A microscope having three-dimensional imaging capability and a three-dimensional microscopic imaging method are provided, the microscope including: at least one excitation device configured to generate a detectable contrast in a detection target region of a sample which is to be detected, in an excitation principal axis direction; at least one detection device, configured to detect the contrast as generated from the detection target region of the sample in a detection principal axis; and at least one movement mechanism, configured to generate a relative movement of the sample relative to the excitation device and the detection device; the relative movement is in a direction neither parallel to nor perpendicular to the excitation principal axis direction or the detection principal axis direction.

A system having a macroscopic imager, a microscopic imager, and a stage for moving a substrate supporting ex-vivo tissue with respect to each of the imagers to enable the macroscopic imager to capture macroscopic images, and the microscopic imager to capture optically formed sectional microscopic images on or within the tissue, when presented to the tissue, via the optically transparent material of the substrate. A computer system controls movement of the stage, and receives the macroscopic and microscopic images. A display is provided for displaying the macroscopic and microscopic images when received by the computer system. The tissue is verified as being in an orientation at least substantially flush against the upper surface of the substrate by being in focus in displayed macroscopic images prior to imaging by the microscopic imager, and if needed, any portion of the tissue unfocused is manually positioned until desired tissue orientation is achieved.

An image capturing apparatus includes a stage on which an object is placed, an image capturer, and a controller that relatively moves the stage within a predetermined plane with respect to the image capturer to move a unit image capturing region, and simultaneously causes the image capturer to perform image capturing a plurality of times. The image capturer includes a light source, an objective lens having an optical axis in a direction intersecting the predetermined plane, a multifocal diffractor that generates a plurality of rays of diffracted light including a ray of diffracted light of 0 order from incident light entering through the objective lens, the plurality of rays of diffracted light having focusing positions being different from each other, and an image capturing member that receives each of the plurality of rays of diffracted light in each of a plurality of segment regions defined in a light receiving surface.

A device may capture, using a camera associated with a microscope, a first image of interstitial material associated with a first set of optical fibers in a field of view of the camera. The device may perform a comparison of the first image of interstitial material and a second image of interstitial material associated with a second set of optical fibers. The device may determine that the first set of optical fibers does not include an expected set of optical fibers based on a result of performing the comparison. The device may determine an amount by which to adjust the field of view of the camera based on the result of performing the comparison. The device may perform one or more actions.

A method comprising determining a quantitative dispersion image of an object based on a set of quantitative phase images, each quantitative phase image of the set of quantitative phase images having been obtained with a respective different illumination light wavelength.

An apparatus includes a detection beam path, along which detection radiation is guided, and a means for splitting the detection radiation between first and second detection paths, with a detector being in each detection path. A microlens array is arranged upstream of at least one detector. The first detector has a first spatial resolution, and the second detector has a second spatial resolution that is lower than the first spatial resolution. Also, the first detector has a first temporal resolution, and the second detector has a second temporal resolution that is higher than the first temporal resolution. Captured image data and computationally combined to form a three-dimensionally resolved resulting image.

Arrangements and methods are provided for obtaining information associated with an anatomical sample. For example, at least one first electro-magnetic radiation can be provided to the anatomical sample so as to generate at least one acoustic wave in the anatomical sample. At least one second electro-magnetic radiation can be produced based on the acoustic wave. At least one portion of at least one second electro-magnetic radiation can be provided so as to determine information associated with at least one portion of the anatomical sample. In addition, the information based on data associated with the second electro-magnetic radiation can be analyzed. The first electro-magnetic radiation may include at least one first magnitude and at least one first frequency. The second electro-magnetic radiation can include at least one second magnitude and at least one second frequency. The data may relate to a first difference between the first and second magnitudes and/or a second difference between the first and second frequencies. The second difference may be approximately between −100 GHz and 100 GHz, excluding zero.

A method and apparatus for microscopic imaging is provided. The method includes: illuminating the sample with illumination radiation to stimulate the detection radiation; capturing the detection radiation from the sample; with the intensity data of the detection radiation from the sample; applying the calibration algorithm to the captured image(s) to acquire the processed second image; the resolution of the processed second image is higher than the acquired first image.

A device may capture, using a camera associated with a microscope, a first image of interstitial material associated with a first set of optical fibers in a field of view of the camera. The device may perform a comparison of the first image of interstitial material and a second image of interstitial material associated with a second set of optical fibers. The device may determine that the first set of optical fibers does not include an expected set of optical fibers based on a result of performing the comparison. The device may determine an amount by which to adjust the field of view of the camera based on the result of performing the comparison. The device may perform one or more actions.