A microscope, method and computer program for obtaining quantitative phase images by digital holographic microscopy. The microscope includes: a coherent light source (1) and a beam splitter (3) for generating an object beam (Lo) for illuminating a sample, and a reference beam (Lr); an optical system with a main optical path making up a telecentric afocal system, and a reference optical path; and recording means (12) recording a hologram of said sample in the image plane of the optical system. The method includes recording a hologram in the image plane of an optical telecentric afocal system. The computer program is adapted for implementing part of the steps of the method.

Apparatus and methods for coherent diffractive imaging with arbitrary angle of illumination incidence utilize a method of fast remapping of a detected diffraction intensity pattern from a detector pixel array (initial grid) to a uniform spatial frequency grid (final grid) chosen to allow for FFT on the remapped pattern. This is accomplished by remapping the initial grid to an intermediate grid chosen to result in a final grid that is linear in spatial frequency. The initial grid is remapped (generally by interpolation) to the intermediate grid that is calculated to correspond to the final grid. In general, the initial grid (x,y) is uniform in space, the intermediate grid ({tilde over (x)},{tilde over (y)}) is non-uniform in spatial frequency, and the final grid ({tilde over (f)}.sub.x,{tilde over (f)}.sub.y) is uniform in spatial frequency.

An image capturing apparatus (500) capable of performing three-dimensional tomography of an object by digital holography, includes a splitting element (502) which splits a light beam emitted from a light source into an object light beam and a reference light beam, an illumination system (503) which controls a plurality of object light beams that are generated from the object light beam and that move in directions different from each other to be incident on the object simultaneously, a composite element (507) which causes the plurality of object light beams to interfere with the reference light beam, an image sensor (508) which acquires hologram generated by interference of each of the plurality of light beams with the reference light beam, and a controller (509) which controls the illumination system so that the plurality of object light beams interfere with each other on the image sensor.

A system and methods for wide field of view, high resolution Fourier ptychographic microscopic imaging with a programmable LED array light source. The individual lights in the LED array can be actuated according to random, non-random and hybrid random and non-random illumination strategies to produce high resolution images with fast acquisition speeds. The methods greatly reduce the acquisition time and number of images captured compared to conventional sequential scans. The methods also provide for fast, wide field 3D imaging of thick biological samples.

The imaging apparatus includes an optical system dividing light into object and reference beams and causing the object beam and the reference beam to interfere with each other to form interference fringes on an image sensor. A processor performs multiple imaging processes for the interference fringes with different incident angles of the object beam to an object, a first process to acquire a transmitted wavefront for each incident angle and a second process to calculate a three-dimensional refractive index distribution from the transmitted wavefronts. The apparatus includes a modulator changing a phase distribution of light in any one of an optical path from a light source to a dividing element, a reference beam path and an optical path from a combining element to the image sensor and causes the modulator to change the phase distribution in at least one of the multiple imaging processes.

The present method includes a data acquisition process and tomographic image generation processes. In the data acquisition process, holograms of an object light and so forth are acquired for each light with a wavelength by changing the wavelengths of the illumination light, off-axis spherical wave reference light, and inline spherical wave reference light. In the tomographic image generation process, a reconstructed light wave of the object light and a reconstructed light wave of the illumination light on a reconstruction surface are generated from these holograms. A reconstruction light wave with adjusted phase is added up for each wavelength to generate a tomographic hologram. From this, an accurate and focused tomographic image without distortion can be generated.

The disclosed invention describes a new apparatus performing a new data acquisition for quantitative refractive index tomography. It is based on a linear scanning of the specimen, opposed to the classical approaches based on rotations of either the sample or the illumination beam, which are based on the illumination with plane waves, which orientation is successively modified in order to acquire angular information. On the contrary, the inventive apparatus and method rely on a specially shaped illumination, which provides straightforwardly an angular distribution in the illumination of the specimen. The specimen can thus be linearly scanned in the object plane in order to acquire the data set enabling tomographic reconstruction, where the different positions directly possess the information on various angles for the incoming wave vectors.

The techniques, apparatus, material and systems are described for a portable camera device which can be attached to the camera port of a conventional transmission or reflection microscope for complex wave front analysis. At least one holographic element (BS, grating) splits the beam (s) containing the sample information in two beams (r,o) and filters (r, o) them. The proposed invention has a relaxed alignment sensitivity to displacement of the beam coming from the microscope. Besides since it compensates the coherence plane tilt angle between reference and object arms, it allows for creating high-visibility interference over the entire field of view. The full-field off-axis holograms provide the whole sample information.

Various systems for imaging are provided. An electronic device includes a biometric sensor and a processing system. The biometric sensor includes an illuminator, a mirror, a biometric sensor array and a beam splitter. The beam splitter splits a beam received from the illuminator into a first beam incident on a biometric sensing area and a second beam incident on the mirror. The beam splitter combines the reflected beams from the biometric sensing area and the mirror. Thereafter, the processing system receives data from the sensor array and reconstructs the biometric image.

An optical measurement system includes a first light source that generates near infrared rays, a silicon-based image sensor, and an optical system including a beam splitter that divides light from the first light source into first light and second light. The optical system records with the image sensor, a first hologram resulting from modulation with second light, of light obtained by illumination of a sample with the first light, the second light being diverging light.