A system and method to produce a hologram of a single plane of a three dimensional object includes an electromagnetic radiation assembly to elicit electromagnetic radiation from a single plane of said object, and an assembly to direct the elicited electromagnetic radiation toward a hologram-forming assembly. The hologram-forming assembly creates a hologram that is recorded by an image capture assembly and then further processed to create maximum resolution images free of an inherent holographic artifact.

Object interference in biological samples generated by lateral shearing interference microscopes is addressed by a shearing microscope slide comprising a periodic structure having alternating reference and sample regions. In some embodiments, the reference regions are configured to provide references that remove sample overlap in a sheared microscopic measurement. A system for generating sheared microscopic measurements is also provided that comprises an inlet configured to receive a sample material, an outlet configured to release a portion of the sample material, and a periodic structure having a plurality of interleaved reference and sample channels. In some cases, the sample channels are configured to accommodate a flow of sample material from the inlet to the outlet and the reference channels are configured to provide references that remove sample overlap in a sheared microscopic measurement.

A holographic three-dimensional multi-spot light stimulation device is provided with: a three-dimensional imaging holographic optical system A which employs fluorescent exciting light to acquire three-dimensional fluorescence distribution information resulting from fluorescent signal light from a plurality of stimulation target objects; and a three-dimensional light stimulation holographic optical system B which employs a light stimulation hologram generated on the basis of the acquired three-dimensional fluorescence distribution information to form a plurality of light spots in space, to impart stimulation simultaneously to the plurality of stimulation target objects. Furthermore, the three-dimensional light stimulation holographic optical system B is provided with a spatial light phase modulating element 22 and a control unit 25, wherein the control unit 25 generates the light stimulation hologram by controlling the spatial light phase modulating element 22 on the basis of the three-dimensional fluorescence distribution information.

A method of producing information from at least one camera image of an object, including: A) recording raw image data of the at least one camera image, B) evaluating the raw image data by a mathematical linkage to produce combination image data, C) deriving the information from the combination image data, D) outputting the information, E) determining an actual measure for a data quality of the raw image data prior to or after evaluation steps in step B), F) determining a deviation between the actual measure for the data quality and a target measure for the data quality of the raw image data of at least one camera image, and G) again recording all raw image data of those camera images, for which the deviation determined in step F) is greater than a predetermined threshold value and repeating at least one evaluation step from step B) and steps C) to F) either until the deviation determined in step F) for the raw image data of all camera images from the plurality of camera images is less than the threshold value or until a predetermined termination condition is fulfilled.

The present disclosure provides a method and apparatus for generating a full-color holographic image. The method of generating a full-color holographic image includes forming images for each color channel based on complex hologram data extracted from rays propagating from a target object, and combining the formed images into one color image, wherein the images for each color channel are formed at reconstruction points for each color channel derived based on the complex hologram data.

A single-shot Fresnel non-coherent correlation digital holographic device based on a polarization-oriented planar lens, comprising: A polarization-oriented planar lens (1) for wavefront modulation and beam splitting, a focusing element (2), a half-wave plate (3) with a small hole and a polarization imaging camera (4). Incident light passes through the polarization-oriented planar lens (1) and the focusing element (2) and is divided into two beams with different polarizations, that is, focused and parallel or focused and divergent beams, wherein the focused beam passes through the small hole of the half-wave plate (3), the parallel or divergent beam passes through the half-wave plate (3), so as to make the polarization of the two beams consistent behind pass through the half-wave plate (3).

An apparatus and method to produce a hologram of an object includes an electromagnetic radiation assembly configured to receive a received electromagnetic radiation, such as light, from the object. The electromagnetic radiation assembly is further configured to diffract the received electromagnetic radiation and transmit a diffracted electromagnetic radiation. An image capture assembly is configured to capture an image of the diffracted electromagnetic radiation and produce the hologram of the object from the captured image.

A system and method to produce a hologram of a single plane of a three dimensional object includes an electromagnetic radiation assembly to elicit electromagnetic radiation from a single plane of said object, and an assembly to direct the elicited electromagnetic radiation toward a hologram-forming assembly. The hologram-forming assembly creates a hologram that is recorded by an image capture assembly and then further processed to create maximum resolution images free of an inherent holographic artifact.

A self-interference digital holographic system obtains interference patterns of incident light using a simple geometric phase lens, and obtains a holographic image of a target object using the interference patterns. The self-interference digital holographic is fabricated simply in a low cost and in a miniaturized size, and the use thereof as actual products is extended to a wide range of applications. The phase of incident light is be changed by rotating a polarizer, independently of a change in the optical path. Phase-shifting effects are obtained with fewer errors in all wavelength ranges, and a more accurate holographic image is produced. A single birefringence hologram is obtained by a one-time image-capturing process by simultaneously forming interference patterns from phase-shifted linearly-polarized beams by space division, using a phase shifter on the basis of space division. Moving holographic images can be captured.

A hologram image acquiring apparatus includes a linear polarizer that filters incident light reflected by an object into a polarized component of a specific angle; a spherical lens that partially converts light that is incident through the linear polarizer to a spherical waveform; and a phase shifter that converts a part of the light incident through the spherical lens to a plane waveform having a respective phase per pixel unit.