A target substance detection method includes forming a complex by causing a target substance and a dielectric particle to bind to each other, the dielectric particle being modified with a substance having a property of specifically binding to the target substance; separating the complex and an unbound particle from each other in a liquid by dielectrophoresis, the unbound particle being a dielectric particle not constituting the complex; and detecting the target substance included in the separated complex by using an imaging element.

An analytical device including an optically opaque cladding, a sequencing layer including a substrate disposed below the cladding, and a waveguide assembly for receiving optical illumination and introducing illumination into the device. The illumination may be received from a top, a side edge, and a bottom of the device. The waveguide assembly may include a nanoscale aperture disposed in the substrate and extending through the cladding. The aperture defines a reaction cell for receiving a set of reactants. In various aspects, the device includes a sensor element and the illumination pathway is through the sensor element. Waveguides and illumination devices, such as plasmonic illumination devices, are also disclosed. Methods for forming and operating the devices are also disclosed.

An optical sensor is used to detect a target in a sample liquid. The optical sensor has an optical sensing layer including, in the same layer, a fluorescent reporter, a fluorescent reference, and an optical isolating reagent. The optical sensing layer has an outer surface that contacts the sample liquid and an opposing surface through which a light source irradiates the optical sensing layer with excitation light, and a detector detects fluorescence emitted from within the optical sensing layer. The optical isolating reagent reduces the amount of (i) excitation light that passes through the optical sensing layer and reaches the sample liquid and (ii) background fluorescence that is emitted within the sample liquid and passes back through the optical sensing layer to the detector. Accordingly, the optical reporter and optical reference fluorescence can be detected with higher signal to noise ratios than in the absence of the optical isolating reagent.

The present disclosure relates to an electrochemical sensor for determining a measurand correlating with a concentration of an analyte in a measuring fluid, comprising: a sensor membrane designed to be in contact with the measuring fluid for detecting measured values of the measurand; a probe housing which has at least one immersion region designed for immersion into the measuring fluid, wherein the sensor membrane is arranged in the immersion region of the probe housing; and a measurement circuit which is at least partially contained in the probe housing and is designed to generate and output a measurement signal dependent on the measurand, wherein the sensor membrane contains an optically detectable substance for marking the sensor membrane.

A sensor membrane for an optical sensor, wherein the outer layer in contact with the medium and/or a layer adjacent thereto has a graft copolymer to form an omniphobic surface in contact with the medium, as well as a sensor cap and/or an optical sensor and a method for manufacturing the sensor membrane.

A fiber optic analyte sensing needle 10 with a photoluminescent analyte-sensitive probe 70 nonadherently entrapped within the lumen 29 of the needle 20 between the distal tip 51 of a fiber optic filament 50 and the distal tip 21 of the needle 20. The probe 70 has unimpeded fluid communication with the external environment through a port 28 in the needle 20.

The present invention provides a fluorescent dye HPTS-lipo for monitoring two-dimensional pH value, a fluorescent sensing film, and use thereof, belonging to the field of two-dimensional pH value monitoring. The fluorescence sensing film for monitoring two-dimensional pH is prepared from the fluorescent dye HPTS-lipo, and HPTS-lipo is embedded by hydrogel D4 in a preparation process. The fluorescent dye HPTS-lipo is prepared by ligating alkylamine into sulfonic acid groups of the fluorescent dye HPTS. Compared with the fluorescent dye HPTS, the pKa value of the modified fluorescent dye HPTS-lipo is significantly varied, so that the fluorescent sensing film is suitable for monitoring different pH values and meets different experimental requirements; and the fluorescent dye HPTS-lipo has better hydrophobicity, can be kept for longer time in environment, and further solves the problem of dye leakage after being embedded by the hydrogel.

In one example of the description, a device may have an elongated body defining a lumen. The elongated body may have a proximal portion and a distal portion. An anchoring member may be positioned on the proximal portion of the elongated body. A first temperature sensor may sense a first temperature of a fluid at a first location in the lumen. A second temperature sensor may sense a second temperature of the fluid at a second location in the lumen. The first location may be proximal to the second location. A heating member may be located proximal to the second temperature sensor. The heating member may heat the fluid within the lumen.

An optochemical sensor for determining a measurement signal, which correlates with a concentration of an analyte of a measuring fluid, comprises: a sensor cap including a sensor spot, wherein the sensor spot contains at least one analyte-sensitive indicator dye and a state description indicator which reflects the aging state of the sensor spot; a radiation source to radiate excitation radiation onto the sensor spot and to excite a luminescence of the indicator dye; a radiation receiver to receive reception radiation emitted by the sensor spot; and a sensor circuit electrically connected to the radiation source and the radiation receiver and configured to control the radiation source and to generate, based on an intensity of luminescence and/or a phase angle of luminescence, a measurement signal representing the concentration of the analyte in the measuring fluid in contact with the sensor spot.

Systems and methods for optimizing detection of optical signals indicating the presence of an analyte of interest in a blood sample are described. In one aspect, a blood culture test vial having a sensor is inoculated with the blood sample, light at an excitation frequency of the sensor is transmitted to the test vial, an intensity of a plurality of fluorescence signals emitted from the test vial is measured, and the plurality of measured fluorescence signals are normalized using by a reference signal that is not dependent on a measured intensity of a fluorescence signal emitted from the test vial. In another aspect, a measurement system measures fluorescence signals from one or more reference vials performing in extreme pH conditions. Fluorescence signals emitted from test vials inoculated with samples under test are measured and compared to the signals measured from the one or more reference vials to address or mitigate variability in hardware components of the measurement system.