An analytical device including an optically opaque cladding, a sequencing layer including a substrate disposed below the cladding, and a waveguide assembly for receiving optical illumination and introducing illumination into the device. The illumination may be received from a top, a side edge, and a bottom of the device. The waveguide assembly may include a nanoscale aperture disposed in the substrate and extending through the cladding. The aperture defines a reaction cell for receiving a set of reactants. In various aspects, the device includes a sensor element and the illumination pathway is through the sensor element. Waveguides and illumination devices, such as plasmonic illumination devices, are also disclosed. Methods for forming and operating the devices are also disclosed.

A method of quantifying the amount of glucose in a sample is provided herein that may further comprise an interferent such as mannitol. At least two measurements are obtained using measurement methods that differ in their sensitivity to the amount of interferent in the sample, thus enabling the results to be compared to determine whether any interferent is present in the sample. A glucose sensor for carrying out a method described herein is also provided

An optochemical sensor for measuring luminescing analytes in a measurement medium includes a sensor housing, a light source, a functional element, a photodetector and a control unit, wherein the sensor housing includes a window suitable for coming into contact with the measurement medium, wherein the light source is configured to emit a stimulation signal in such a way that the stimulation signal is partially emitted onto the functional element and the stimulation signal is partially emitted through the window into the measurement medium as to stimulate a first analyte present in the measurement medium, wherein the functional element includes a reference dye that includes an inorganic material and is suitable for emitting a first luminescence signal upon stimulation with the first stimulation signal.

A disposable measurement tip for an instrument for measuring at least one parameter of a sample, the measurement tip comprising; a tip body; a filling channel in the tip body for holding the sample to be analyzed and having an opening on one end of the tip body to receive the sample; a population of micro-particles held within the tip body filling channel and which can be mixed with the sample when the sample is received in the filling channel; wherein in use the sample in the tip is illuminated by the instrument and the illumination which impinges on the micro-particles is directed back, by the micro-particles, to the instrument to be detected and to thereby enable the at least one parameter to be determined.

A sensor (202) uses an optical-sensing technique for determining more than one parameter in a measurement medium (204). The sensor has an optochemical sensitive element (208) with a first sensing layer (228) and a second sensing layer (230). Each of the sensing layers is arranged on a substrate (222). The first sensing layer has a first indicator (236) that determines a first parameter and the second sensing layer has a second indicator (238) that determines a second parameter in the measurement medium.

An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed.

The present application discloses a sensor for determining a measurand correlated with a concentration of at least one analyte belonging to the class of reactive oxygen species in a measuring fluid, the sensor including a sensor element having an indicator substance, wherein the indicator substance is oxidized into an oxidized form of the indicator substance by the at least one analyte, a means for generating a flow of current in the sensor element that causes a reduction of the oxidized form of the indicator substance and thereby regeneration of the indicator substance, an optical measuring sensor to detect measuring radiation influenced by the oxidized form of the indicator substance and to generate an electrical measuring signal using the influenced measuring radiation, and a sensor switch connected to the optical measuring sensor to receive the measuring signal and to ascertain a measured value of the measurand using the measuring signal.

An optical glucose sensor for detecting and/or quantifying the amount of glucose in a sample comprising: a sensing region comprising a boronic acid receptor for binding to glucose and a fluorophore associated with said receptor; an optical waveguide for directing incident light onto the sensing region; and a glucose-permeable barrier layer comprising a semi-permeable membrane having pores and a hydrophilic polymer within the pores of the semi-permeable membrane, the barrier layer overlying at least a part of the sensing region;
wherein the sensor is adapted so that glucose enters the sensing region of the sensor through the glucose-permeable barrier layer, and an ROS-quenching agent is present in the sensing region and/or the glucose-permeable barrier layer.

According to one aspect, the invention relates to an aquatic sample analysis system adapted for in situ use. The system includes an incubation chamber having an optically clear portion and forming an opening for receiving a fluidic sample and apparatus for sealing the opening. The system also includes a sensor for sensing at least one parameter associated with the sample inside the chamber, a control module in communication with the sensor, and a power source.

A pH micro-probe, a temperature micro-probe, and an immuno-based micro-probe each include a shaft for transmuting an input light signal and a tip for inserting into a cell or other substance for measuring pH, temperature, and/or antigens. The pH micro-probe and the temperature micro-probe each include a luminescent material positioned on the tip of the micro-probe. The light signal excites the luminescent material so that the luminescent material emits a luminescent light signal. The luminescent light signal has a property value dependent on the pH or temperature being measured and reflects back through the shaft for being measured by a light signal measuring device. The immuno-based micro-probe includes a reflective material that has an effective refractive index dependent on the number of antigen-antibody bonds present on the reflective material.