A solution is proposed for testing a luminescence imaging apparatus (105). A corresponding testing device (110) comprises one or more seats (320) and one or more containers (325), each filled with a liquid comprising at least one luminescence substance and accommodated in a corresponding seat (320); the seats (320) have corresponding windows (330) for imaging the luminescence substance of the containers (325) accommodated therein. The seats (320) are slanted with respect to a resting surface (310) of the testing device (110). A holder (305) for use in the testing device (100) is further provided. A luminescence imaging apparatus (105) for use with the testing device (110) is also proposed. Moreover, a system (100) comprising a luminescence imaging apparatus (105) and this testing device (110) is proposed.

A solution is proposed for testing a luminescence imaging apparatus (105). A corresponding method (700) comprises acquiring (706) a photograph image and finding (708) a position of the testing device (110) in the photograph image. The 5method further comprises acquiring (706) a luminescence image and determining (734-736) a representation of sites of the testing device (110), each comprising at least one luminescence substance, in the luminescence image according to the position of the testing device (110) in the photograph image. The luminescence imaging apparatus (105) is then tested (754-772) according to the representation of the sites (330) in the 0luminescence image. A corresponding computer program (600) and a computer program product for implementing the method (700) are also proposed. Moreover, a testing device (110) for use in the method (700) is proposed.

Methods and apparatus are presented for measuring a photoluminescence (PL) response, preferably a spatially resolved image of a PL response, from an object exposed to solar irradiation. In certain embodiments signals from the object are measured in two or more different spectral bands selected such that one of the measured signals has a higher PL component relative to ambient reflectance compared to another measured signal, enabling the PL component to be enhanced by a suitable differencing procedure. In other embodiments a signal from an object is measured in a spectral band selected such that at least 20% of the measured signal comprises PL generated from the object by the solar irradiation. The methods and apparatus have particular application to outdoor inspection of photovoltaic modules without having to modulate the operating point of the modules.

A microfluidic sensing assembly may include a first structure supporting a sensor array, a second structure joined to the first structure and forming a microfluidic passage and a flat lens to focus light, following reflection of the light back and forth across the microfluidic passage, from the microfluidic passage onto the sensor array.

A nucleic acid sequence measuring apparatus (1) measures a target (TG) having a specific nucleic acid sequence included in a sample. The nucleic acid sequence measuring apparatus (1) includes a detector (12) configured to detect fluorescence emitted from a nucleic acid sequence measuring device (DV) which emits fluorescence due to an addition of the target (TG), and a calculator (25) configured to measure the target based on a difference between a first amount of light indicating an amount of fluorescent light emitted from a predefined measurement region of the nucleic acid sequence measuring device (DV) at a first time point before or immediately after an addition of the sample to the nucleic acid sequence measuring device (DV) and a second amount of light indicating an amount of fluorescent light emitted from the measurement region at a second time point after a predefined time has elapsed from the addition of the sample to the nucleic acid sequence measuring device (DV), based on a detection result of the detector (12).

A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir; (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other.

An improved system and method for reading an upconversion response from nanoparticle inks is provided. A is adapted to direct a near-infrared excitation wavelength at a readable indicia, resulting in a near-infrared emission wavelength created by the upconverting nanoparticle inks. A short pass filter may filter the near-infrared excitation wavelength. A camera is in operable communication with the short pass filter and receives the near-infrared emission wavelength of the readable indicia. The system may further include an integrated circuit adapted to receive the near-infrared emission wavelength from the camera and generate a corresponding signal. A readable application may be in operable communication with the integrated circuit. The readable application receives the corresponding signal, manipulates the signal, decodes the signal into an output, and displays and/or stores the output.

Techniques are described for dynamically correcting image distortion during imaging of a patterned sample having repeating spots. Different sets of image distortion correction coefficients may be calculated for different regions of a sample during a first imaging cycle of a multicycle imaging run and subsequently applied in real time to image data generated during subsequent cycles. In one implementation, image distortion correction coefficients may be calculated for an image of a patterned sample having repeated spots by: estimating an affine transform of the image; sharpening the image; and iteratively searching for an optimal set of distortion correction coefficients for the sharpened image, where iteratively searching for the optimal set of distortion correction coefficients for the sharpened image includes calculating a mean chastity for spot locations in the image, and where the estimated affine transform is applied during each iteration of the search.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

Various embodiments may provide a method of illuminating a sample plane. The method may include providing an illumination subsystem, the illumination subsystem including an optical source and at least one lens, having an optic axis at an incident angle greater than 0° and less than 90° to a normal of the sample plane. The method may also include rotating the illumination subsystem about a pivot point between the optical source and the sample plane along the optic axis so that an adjusted illumination distribution generated by the illumination subsystem at the sample plane has greater symmetry compared to a reference illumination distribution generated by the illumination subsystem at the sample plane without the rotation about the pivot point.