A spectroscopic measurement apparatus includes a light source, an integrator, a spectroscopic detector, and an analysis unit. The integrator includes an internal space in which a measurement object is disposed, a light input portion for inputting light to the internal space, a light output portion for outputting light from the internal space, a sample attachment portion for attaching the measurement object, and a filter attachment portion for attaching a filter unit. The filter unit has a transmission spectrum in which an attenuation rate for excitation light is larger than an attenuation rate for up-conversion light, and attenuates the light output from the light output portion. The analysis unit analyzes luminous efficiency of the measurement object on the basis of the transmission spectrum data and the spectroscopic spectrum data acquired by the spectroscopic detector.

Provided is a biological tissue observation apparatus including an optical fiber probe that receives fluorescence via an input end disposed at one end thereof and that optically guides the fluorescence toward the base end in the longitudinal direction, a photodetector that is disposed facing an output end disposed at the base end of the optical fiber probe and that detects the fluorescence output from the output end, and a scatter suppressing unit provided near the input end of the optical fiber probe.

Provided herein are devices, systems, and methods for characterizing a biological sample in vivo or ex vivo in real-time using time-resolved spectroscopy. A light source generates a light pulse or continuous light wave and excites the biological sample, inducing a responsive fluorescent signal. A demultiplexer splits the signal into spectral bands and a time delay is applied to the spectral bands so as to capture data with a detector from multiple spectral bands from a single excitation pulse. The biological sample is characterized by analyzing the fluorescence intensity magnitude and/or decay of the spectral bands. The sample may comprise one or more exogenous or endogenous fluorophore. The device may be a two-piece probe with a detachable, disposable distal end. The systems may combine fluorescence spectroscopy with other optical spectroscopy or imaging modalities. The light pulse may be focused at a single focal point or scanned or patterned across an area.

Analyte collection and testing systems and methods, and more particularly to testing systems and methods that achieve significant improvements in the detection of fluorescence signals in the reader by modulating the applied optical excitation. Also described herein are optical detection apparatuses and methods for removable photonic chips that do not require translation for calibration when coupling the photonics chip with the sensing system. Also described herein are methods and apparatuses for accurately calibrating a dilution factor when reading from a photonics chip.

Analyte arrays such as solutes in a slab-shaped gel following electrophoresis, and particularly arrays that are in excess of 3 cm square and up to 25 cm square and higher, are imaged at distances of 5 cm or less by either forming sub-images of the entire array and stitching together the sub-images by computer-based stitching technology, or by using an array of thin-film photoresponsive elements that is coextensive with the analyte array to form a single image of the array.

The present application describes an optical sensor for measuring oxygen gas levels in a medium. The optical sensor includes a substrate having a first and second surface. The optical sensor also includes a first coating applied on the first surface of the substrate. The first coating may include an oxygen impermeable matrix doped with a first fluorophore. The optical sensor may include a second coating applied on the substrate. The present application also describes a capnography system for measuring oxygen including an optical sensor and an algorithm to estimate the maxima of oxygen levels from instantaneous oxygen levels and calculating instantaneous carbon dioxide levels from the difference between average maximum oxygen gas level and instantaneous oxygen gas level.

An orientation characteristic measurement system includes an irradiation optical system, a detection optical system, a light detector, a rotation mechanism changing an angle (ϕ) between a surface of the sample and an optical axis (L2) of the detection optical system, and a computer, and the computer includes a rotation mechanism control unit controlling the rotation mechanism, a distribution acquisition unit normalizing an angle-dependent distribution of light intensity to acquire an angle-dependent distribution of light intensity, an area specifying unit specifying light intensity in a maximum area on the basis of the angle-dependent distribution of the light intensity, and a parameter calculation unit calculating the orientation parameter (S) on the basis of a linear relationship determined using the film thickness and refractive index of the sample and the light intensity in the maximum area.

The invention provides systems for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation and methods for using the same. The system includes a laser source, collection fibers, a demultiplexer and an optical delay device. All references cited herein are incorporated by reference in their entirety as though fully set forth. Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of-ordinary skill in the art in which this invention belongs.

A downhole fluid properties optical analysis probe (1) to analyze at least one property of a multiphase flow mixture (100) flowing in a hydrocarbon well (51) has an elongated cylindrical body shape. It comprises an optical tip (5) at one end of the elongated cylindrical body arranged to be in contact with the multiphase flow mixture (100). It further comprises an optical link (6) adapted for a connection with an electronics module (11) at another end of the elongated cylindrical body arranged to be separated from the multiphase flow mixture (100). The optical tip (5) is coupled to the optical link (6) through a removable and watertight coupling (7). The removable and watertight coupling comprises a first portion (9a) of a protective tube (9) resistant to downhole conditions, said first portion (9a) enclosing the optical link (6) and comprising at least one first ring bulge (22) close to a coupling interface (10), and a second portion (9b) of the protective tube (9) partially enclosing the optical tip (5) such as to let a distal end of the optical tip (5) in contact with the multiphase flow mixture (100), said second portion (9b) comprising at least one second ring bulge (23) close to the coupling interface (10). It further comprises a coupling tube (24) surrounding facing ends of the first portion (9a) and the second portion (9b), said coupling tube (24) being adjusted in size to fit in between said first and second ring bulges (22, 23), and a coupling and protecting sheath (25) enclosing said first ring bulge (22), coupling tube (24) and second ring bulge (23) in a watertight manner.

A spliced detection apparatus for simultaneous wide-range in-situ detection of dissolved oxygen in a sludge-water interface of the present invention comprises a dissolved oxygen shimmer host, several detection probes, a casing and detection kits, several vertically through seat chambers being disposed in the casing, the detection kits can be placed and fixed in the seat chambers, the detection kits may be placed on the sludge-water interface, and each detection kit is provided with a vertically through water passage chamber, while the front side face and the back side face of the detection kit are respectively provided with several front slots and rear slots horizontally extending into the detection kit respectively, the detection apparatus further comprises tabs, with probe grooves being provided on the tabs, each detection probe can be put into the probe groove of the corresponding tab and fixed therein, the tabs may be inserted either into the front slots or the rear slots and can enable the probe tips of the detection probes to be placed in the water passage chamber, the detection apparatus can carry out simultaneous wide-range detection of the dissolved oxygen at different heights and horizontal positions of the sludge-water interface, and thus is quick and convenient for use.