Provided are filter elements or cells and filtration assemblies or capsules for purifying and/or clarifying fluids used during various biopharmaceutical applications, for example, chromatography or depth filtration, and processes for making and using the same. A filter element comprises a media pack comprising two sides and a separator element located between the two sides, at least one side comprising a filtration media, wherein an inner edge periphery and/or an outer edge periphery of the filtration media comprise a bond directly to a portion of the separator element.

A device (201) is provided for collecting a substance from an ambient fluid. The device includes a housing (205) equipped with an (207) inlet and an outlet (209) which are in fluidic communication with each other, and a collector (203) which has a plurality of spaced-apart layers (213). The collector is disposed in a fluidic flow path between the inlet and the outlet such that a flow of fluid passes through adjacent layers of the collector. Each of the layers includes a substrate (217) having a sorbent or adherent (219) disposed thereon, and a plurality of embossments (215) which maintains the layers in the spaced-apart configuration.

A method for detecting urea-formaldehyde resin in melamine-formaldehyde materials is provided. The method includes the following steps: washing samples to be tested with hydrochloric acid solution, placing it at 110? C.?5? C. for hydrolysis for 60 min?5 min, passing through a polyethersulfone filter membrane to obtain the test solution, and detecting the test solution through high-performance liquid chromatography-mass spectrometry.

A device (201) is provided for collecting a substance from an ambient fluid. The device includes a housing (205) equipped with an (207) inlet and an outlet (209) which are in fluidic communication with each other, and a collector (203) which has a plurality of spaced-apart layers (213). The collector is disposed in a fluidic flow path between the inlet and the outlet such that a flow of fluid passes through adjacent layers of the collector. Each of the layers includes a substrate (217) having a sorbent or adherent (219) disposed thereon, and a plurality of embossments (215) which maintains the layers in the spaced-apart configuration.

A pump unit includes a pump and a metal ion trap. The pump includes a metal member that comes into contact with a mobile phase. The pump pumps a mobile phase through a flow path. The metal ion trap includes a filter element. The filter element includes a metal ion retention structure for retaining metal ions by interacting with the metal ions included in a mobile phase. The filter element is provided in the flow path for a mobile phase pumped by the pump. The pump unit may be provided in a chromatograph.

Sample preparation device for receiving in sealing relation one or more filter assemblies and one or more sample containers, each container uniquely positioned within the device to receive filtered sample from a designated filter. The device includes a body, a lid disposed on the body, a container tray, a base, an integrated seal on the body, a valve and a valve actuator. The tray can be disposed in a vacuum chamber defined by the lid and the body, and one or more sample containers can be disposed in the tray. When properly positioned in the tray in the vacuum chamber, each container is in fluid communication with a single respective filter assembly disposed in sealing relation in a respective aperture in the lid. A single actuation of a valve to place the vacuum chamber under vacuum causes the lid to seal to the base and drives the simultaneous filtration of a plurality of samples.

A liquid phase microextraction (LPME) method was developed for the determination of sulfur compounds in crude oils and its fractions (e.g diesel). Wide ranges of sulfur compounds including benzothiophene, dibenzothiophene and their derivatives were used as model compounds for extraction. After extraction, the analyses were performed by gas chromatography equipped with sulfur chemiluminescence detector (GC-SCD). Various experiments conditions influencing the extraction such as extraction times, extraction solvents, solvent volume and quantitative parameters were optimized to achieve higher extraction efficiency and high accuracy. The optimized conditions were applied to the determination of sulfur compounds in crude oil and diesel.

Disclosed is a method for removing factor XI (FXI) during plasma protein purification, more specifically a method for removing FXI including dialyzing and concentrating a plasma protein fraction II paste containing FXI and a plasma protein, and then removing the FXI using ceramic-based cation exchange resin. The method for removing factor XI (FXI) can improve removal efficiency of impurities and thrombogenic substances, thereby producing stable plasma proteins with improved quality.

A system and method is provided for validating the manufacturing process for the production of complex biological compositions, and particularly for providing process validation information for evaluation by a federal regulatory agency. The system and method continuously and chronologically assess the concentration of proteoforms within the biological composition as it is being produced in a fermentor. Samples from the fermentor are analyzed in a pre-selected array of analysis columns, with data generated by the columns being accumulated and evaluated, and particularly compared with data from previous stages in the production process. A continuous process validation system includes top-down and bottom-up analysis sectors, each including a plurality of different analysis columns that can be selected by the controller for a particular biological composition and a particular production process.

The present invention discloses a quantitative detection method of multiple metabolic components in a biological sample and a metabolic chip used in the method. The detection method includes performing derivatization treatment on the biological sample and then detecting the derivatized biological sample by liquid chromatography-mass spectrometry. The metabolic chip of the present invention includes a chip carrier microtiter plate and related reagents, and quantitative detection of multiple metabolic components of different magnitudes such as amino acid, phenol, phenyl or benzyl derivative, indole, organic acid, fatty acid, sugar, and bile acid in the biological sample on the same microtiter plate can be achieved.