The present invention relates to a method for separating one or more components from a liquid feed mixture in an EBA-SMB operating mode without the need of pumps at the outlets of the EBA columns. The present invention also relates to a simulated moving bed separation device with expanded bed adsorption columns which can be used in the method according to the invention.

Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication

Go between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

A fractal flow device comprising at least one fractal pack. The at least one fractal pack comprises at least two fractal cells, where each fractal cell comprises a fractal distributor, a chamber adjacent the fractal distributor, and a fractal collector adjacent the chamber. Methods of using the fractal flow device are also disclosed.

A liquid chromatograph analysis method and a liquid chromatograph minimize analysis time when performing analyses by switching columns and mobile phases. Liquid chromatograph 100 for performing a plurality of analyses according to a schedule table includes: mobile phase switching sections 15, 16 for switching a plurality of mobile phases to select a mobile phase to be used in analysis; column switching sections 31, 33 for switching a plurality of columns 32a-32f to select a column to be used in analysis; and control section 60 including memory 61 for storing an equilibration time of each of the plurality of columns 32a to 32f and an equilibration controller 66 for controlling column equilibration. If used columns or used mobile phases are different between two consecutively executed analyses, equilibration controller 66 equilibrates a column used in the later of the two analyses over an equilibration time read out from memory 61.

A liquid chromatograph includes: a concentration column for capturing a target component; an analysis column for separating the target component from other components; first passage-switching units for switching between the state of forming a passage through which a sample-introduction mobile phase containing the liquid sample is fed to the concentration column, and the state of forming a passage through which an eluant for eluting the target component captured in the concentration column is fed to the concentration column; a storage unit for storing the eluant; and a second passage-switching unit for switching between the state of forming a passage through which an analysis mobile phase is fed to the analysis column and a passage through which the eluant from the concentration column is fed to the storage unit, and the state of forming a passage through which analysis mobile phase is fed to the analysis column via the storage unit.

Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.

A liquid chromatograph mass spectrometer specifying a location where a flow path is clogged and recovering in a short time. The liquid chromatograph mass spectrometer includes a first flow path passing through a separation column, a second flow path not passing through the separation column, a mass spectrometry unit on the downstream side of the first and second flow paths and analyzes a sample that has passed through the first flow path, a first valve for connecting any one of the first and second flow paths to the mass spectrometry unit, and a controller for controlling driving of the first valve, connecting the first flow path to the mass spectrometric unit, comparing the measured value of the mass spectrometric unit with a predetermined threshold value, and connecting the second flow path to the mass spectrometry unit when it is determined to be abnormal.

A liquid chromatograph mass spectrometer specifying a location where a flow path is clogged and recovering in a short time. The liquid chromatograph mass spectrometer includes a first flow path passing through a separation column, a second flow path not passing through the separation column, a mass spectrometry unit on the downstream side of the first and second flow paths and analyzes a sample that has passed through the first flow path, a first valve for connecting any one of the first and second flow paths to the mass spectrometry unit, and a controller for controlling driving of the first valve, connecting the first flow path to the mass spectrometric unit, comparing the measured value of the mass spectrometric unit with a predetermined threshold value, and connecting the second flow path to the mass spectrometry unit when it is determined to be abnormal.

Tests of channels of a system as a whole can be easily performed without adding a complicated mechanism. A liquid feeding part includes a liquid feeding channel to feed a mobile phase, a drainage channel to release pressure in the liquid feeding channel, an analysis channel that discharges the mobile phase into the sample introduction part, and a channel switching valve that selectively connects the liquid feeding channel to one of the drainage channel and the analysis channel. The channel switching valve is configured to be able to provide a tight stopper state in which the liquid feeding channel is connected to neither the analysis channel nor the drainage channel.

The disclosed chromatographic system enables sample slices to be moved back and forth between columns to rapidly isolate and measure a single or multiple components in a complex matrix. The independently controlled, two column system allows for the flow-recycling to take place since the sample slice can be effectively halted on either column until the second column is thermally ready to accept it again. The plumbing scheme also allows one to make an infinitely long column by being able to move components back and forth between the two by activating and deactivating the valve at the appropriate times.