Provided is a method for control and/or monitoring and/or optimization of a chromatographic process, in which the method comprises at least 2 columns which are operated, alternatingly, wherein this operation can be carried out in that the at least 2 columns are operated in interconnected and disconnected states, wherein the columns switch positions after such a sequence of interconnected and disconnected state, and wherein downstream of at least one, or of each column, a detector is located capable of detecting the desired product and/or impurities when passing the detector.

A multi-path selector valve used in liquid chromatography and other analytical methods for directing fluid along alternate paths of a flowstream. The selector valve has a stator and a rotor. The dynamic face of the stator has a plurality of openings arranged along an inner ring, a plurality of openings arranged along an outer ring, and an annular collection groove formed in the dynamic face. The inner ring, outer ring, and annular collection groove are concentric circles. The rotor's dynamic face is configured to mate with the rear face of the stator, and has two fluid flow paths. One fluid flow path has one end at a rotational center of the rotor and another end of the fluid flow path is configured to be aligned with a stator opening along the inner ring. The second fluid flow path has one end that is configured to be aligned with the annular collection groove and another end that is configured to be aligned with a stator opening along the outer ring.

The disclosure relates to a gradient twin column recycling chromatography method that is used to separate a mixture containing closely eluting compounds. In one embodiment, a sample includes a primary organic compound and one or more impurities that closely elute with the primary organic compound. A gradient mobile phase is initially used to remove unwanted early eluting and late eluting impurities from the sample. After the gradient removal of some of the impurities is complete, the remaining mixture of the primary organic compound and the closely eluting impurities are separated using recycle chromatography methodology with an isocratic mobile phase.

The invention discloses a flow control block for a stack of chromatography column modules, as well as a stack of chromatography modules comprising at least one flow control block. The flow control block is in a first position or configuration capable of connecting two chromatography column modules, or a chromatography column module and an endpiece, in parallel and in a second position or configuration it is capable of connecting two chromatography column modules, or a chromatography column module and an endpiece, in series.

A system capable of performing both single and multidimensional liquid chromatography includes a solvent delivery system, a sample injection system, a first dimension column path configured to perform a separation process in a first dimension, a second dimension column path configured to perform a separation process in a second dimension that is different than the first dimension, a valve system; and a sample injection system fluidically connected to the valve system. The valve system is configured to direct flow from a sample injection system to a first dimension column path when the valve system is in a first position, and to direct flow from the sample injection system to the second dimension column path without the flow path flowing through the first dimension column path in the chromatography system when the valve system is in a second position.

A multidimensional chromatographic assembly includes a chromatographic medium selector module, which receives a sample from an injector module and moves the sample through one of at least two chromatographic media of a chromatographic medium module using at least one eluent from at least one fluid moving module. At least a portion of the sample is re-circulated through the same or the second chromatographic medium using a multi-configuration fluid diverting module, which isolates a selected portion of the chromatographed eluent containing at least a portion of the sample in at least one fluid holding compartment and later moves the isolated portion through one of the chromatographic media in an iterative manner until all attributes of the isolated portion in question are analyzed. A detector module, which is located between the chromatographic medium selector module and the fluid diverting module, acquires data each time a portion of the sample passes through the detector module and provides data for a multidimensional chromatogram. The configurable portion (the rotor) of the fluid diverting module comprises movable flow-paths with two termini, which lie on a circular perimeter, concentric to the axis of rotation of the rotor, on the interfacial plane where the rotor meets the stationary portion of the fluid diverting module (the stator), and a connecting coplanar groove, spatial disposition of which is either concave or convex to the circular perimeter with only the termini intercepting the perimeter. The entire assembly is controlled by a controller, which receives data from the detector module and sends instructions to all modules during the multidimensional analysis with or without human intervention.

A multiple channel selector valve includes a stator and a rotor that is rotatable with respect to the stator. The rotor face includes first and second fluid flow paths for transferring fluids to selected sets of passages in the stator. The fluid flow paths in the rotor face are specifically configured to accommodate high fluid flow rate regimes while reducing flow restriction.

A range of pH of a mobile phase that is usable for an analysis with use of each column is registered by a pH range registrar in association with identification information of each column. A pH of each mobile phase is registered by a pH registrar. A column to be used for an analysis is selected by a column selector. A chromatograph is controlled by an analysis controller such that a mobile phase having a registered pH that is out of a range of pH that is registered in association with a selected column is not supplied to the selected column. The chromatograph is controlled by the analysis controller such that an analysis is performed while a mobile phase having a registered pH that is in a range of pH that is registered in association with a selected column is supplied to the selected column.

Described herein are systems and methods used for carrying out a two-dimensional liquid chromatography process using size exclusion chromatography as a first dimension.

A system for component interconnection for use in liquid chromatography includes a first switching valve and a second switching valve. A first connecting line fluidly connects the first switching valve to the second switching valve. A second connecting line fluidly connects the first switching valve to the second switching valve. A metering device is located in the first connecting line.