Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.

The present invention relates to a chromatography system wherein the chromatography system comprises an eluting system and a capturing system consisting of at least two chromatography units operated alone or in series and a capturing process employing in-line buffer dilution in, which concentrated buffers are blended with water and provided to the chromatography units.

A valve switching cassette for selectively interconnecting components of a bioprocess installation, wherein the valve switching cassette comprises at least one fluid flow system of ports and fluid lines, which fluid flow system includes primary ports, communicating with primary fluid lines, and secondary ports, communicating with secondary fluid lines, wherein the valve switching cassette comprises an array of switchable valve units for selectively interconnecting the primary fluid lines with the secondary fluid lines via transfer fluid lines. It is prosed, that the valve switching cassette comprises a transfer plate with apertures and an elastically deformable membrane structure on each flat side of the transfer plate, that at least part of the primary fluid lines and secondary fluid lines are extending between the transfer plate and one of the membrane structures and that the transfer fluid lines are at least partly provided by the apertures.

Certain embodiments described herein are directed to chromatography systems that include a microfluidic device. The microfluidic device can be fluidically coupled to a switching valve to provide for selective control of fluid flow in the chromatography system. In some examples, the microfluidic device may include a charging chamber, a bypass restrictor or other features that can provide for added control of the fluid flow in the system. Methods of using the devices and methods of calculating lengths and diameters to provide a desired flow rate are also described.

A chromatography analysis apparatus (30) comprises: a fractionation device (32) for receiving a sample, the fractionation device (32) defining a sample flow path that includes a guard column; and a fractionation output analyser (34), wherein a fractionation output of the guard column is provided to an input of the fractionation output analyser (34) for enabling subsequent analysis of the fractionation output by the fractionation output analyser (34).

The present invention relates to a valve unit for a chromatography apparatus, the valve unit comprising a fluid inlet configured to receive an input fluid, a fluid outlet configured to provide an output fluid, a first pair of fluid ports configured to be coupled to a first column, a second pair of fluid ports configured to be coupled to a second column, a coupling valve assembly configured to direct fluid between a selection of the fluid inlet, the fluid outlet, the first pair of fluid ports and the second pair of fluid ports in response to one or more control signals, wherein the coupling valve assembly is configured to direct fluid using a selection of membrane valves coupled by fluid channels comprised in a body of the coupling valve assembly. The invention further relates to a chromatography apparatus comprising the valve unit and a membrane valve comprised in the valve unit.

An on-line system that performs concentration and solvent exchange in order to improve a detection level of analytes by a liquid chromatography (LC) is provided. The on-line system comprises: a first pump and a second pump for supplying a solvent; a liquid chromatography (LC) including a separation column (SC) connected to the first pump; a trapping column (TC) for collecting the analytes separated from the separation column; a concentration column (CC) for concentrating the analytes collected in the trapping column (TC); a detector; and first to third switching valves that communicate fluid with at least one of the first or second pumps. An analysis method using the same is also provided.

A method for transferring a radioisotope which is fixed on a first stationary phase contained in a first chromatography column to a second stationary phase contained in a second chromatography column, to fix the radioisotope on the second stationary phase, wherein the radioisotope is selected from the radioactive isotopes of thorium, radium, lead, bismuth and uranium, the method comprising at least the following steps: a) eluting the radioisotope from the first stationary phase with an aqueous solution A1 comprising an agent complexing the radioisotope, whereby an aqueous solution A2 which comprises complexes of the radioisotope is obtained; b) dissociating the complexes of the radioisotope present in the aqueous solution A2 by modifying the pH of the aqueous solution A2, whereby an aqueous solution A3 comprising the decomplexed radioisotope is obtained; c) loading the second stationary phase with the aqueous solution A3; and d) washing at least one the second stationary phase with an aqueous solution A4.

Described are multidimensional liquid chromatography (MDLC) systems and methods of performing MDLC. The MDLC systems includes valve configurations that enable a flow containing analytes in the eluent of a first dimension liquid chromatography system to be modulated such that the analytes can be captured in fluidic loops and subsequently provided to a second dimension liquid chromatography system. Various system embodiments are provided in a smaller instrument size, include an option to use the same detector for both first and second dimensions, enable improvement in valve timing operations and provide a reduction in the magnitude and effects of pressure pulses. In addition, the modulator can enable dilution of the analytes and enable incompatible mobile phases and mobile phase flow rates to be used in the first and second dimensions.

A method includes supplying a reagent to a column, where the column is configured to purify an element from a sample matrix for isotopic analysis. The method also includes loading the column with the sample matrix, and supplying a second reagent to collect the element retained by the column. The method further includes loading the column with a second sample mixture, and collecting an element from the second sample mixture retained by the column. A column configured to separate an element from a sample matrix for isotopic analysis includes a resin configured to retain the element. The column also includes a first frit disposed of a first end of the column and a second frit disposed of a second end of the column. The column is configured to receive a first reagent in a first flow direction and a second reagent in a second flow direction different from the first flow direction.