Disclosed herein are stable and versatile protein nanoparticles having a range of tunable fluorescent properties. Such nanoparticles may find utility in biological imaging. Methods of synthesis of such nanoparticles are also disclosed.

Methods of cancer treatment are disclosed. Particularly, disclosed herein are methods of administering LAL and PPAR ligands for treating various cancer patients to promote anti-cancer immunity immunity (suppress MDSCs), use for inhibiting tumor progression, and use for inhibiting tumor metastasis.

Monoclonal and polyclonal antibodies that bind hamster phospholipase B-like 2 are provided. Also provided are methods for detecting and quantifying hamster phospholipase B-like 2, for example, in recombinant polypeptide preparations, as well as kits for carrying out such methods. Methods of screening or selecting host cell lines or recombinant polypeptide-expressing cell lines that express low levels of hamster phospholipase B-like 2 are also provided.

Monoclonal and polyclonal antibodies that bind hamster phospholipase B-like 2 are provided. Also provided are methods for detecting and quantifying hamster phospholipase B-like 2, for example, in recombinant polypeptide preparations, as well as kits for carrying out such methods. Methods of screening or selecting host cell lines or recombinant polypeptide-expressing cell lines that express low levels of hamster phospholipase B-like 2 are also provided.

The invention provides new reversible monoacylglycerol lipase (MAGL) inhibitors that are useful for the treatment or prophylaxis of diseases or conditions associated with MAGL. The reversible MAGL inhibitors according to the present invention may also be labeled with radioisotopes and are thus useful for medical imaging, such as positron-emission tomography (PET) and/or autoradiography.

The present disclosure relates to an organism trapping device that includes a vibration wave generator configured to generate frequency ranges from 1.00 to 1.67 Hz (Hertz), an inciting engine communicatively coupled with the vibration wave generator, and configured to generate signals representing vibration parameters based on the generated frequency, a walled container operatively coupled with the inciting engine and adapted to contain fluid and receives the generated signals to simulate flow of blood in human blood vessels based on the vibration parameters to attract number of organisms towards the device, a fumigating agent adapted to compress the number of organisms entering into the device, sensors configured to sense an entry of number of organisms into the device, and a processor communicatively coupled with the sensor and activates the fumigating agent based on the signal received from the sensor. The present disclosure also relates to method of trapping organisms.

Methods of mitigating lipoprotein interference in in vitro diagnostic assays for target hydrophobic analytes are disclosed, as well as compositions, kits, and devices useful in said methods. A pretreatment reagent is utilized that includes at least one enzyme that digests lipoprotein.

This invention provides a substrate solution for measuring lipase activity, a reagent for measuring lipase activity, and an emulsion solution excellent in storage stability. This invention also provides a method for measuring lipase activity in a sample that enables accurate measurement over a long period of time. This invention further provides a method for improving storage stability of a substrate solution for measuring lipase activity and an emulsion solution. To this end, a substrate solution for measuring lipase activity comprising the emulsion solution comprising micelle particles of 1,2-o-dilauryl-rac-glycero-3-glutaric acid (6-methylresorufin) ester and side-chain-type nonreactive polyether-modified-type modified silicone oil is used.

Disclosed are methods of measuring enzyme activity in a sample. The methods use disulfide-containing detection reagents with an electrochemiluminescent functional group.

The present invention provides methods for quantifying an amount of plasmalogens in samples with high accuracy in an easy, convenient and inexpensive manner by using a hydrolysis processing to samples and a lipid extraction followed by the measurement using High Performance Liquid Chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) or Mass Spectrometer (MS), a fluorescence plate reader or a plate reader. The present invention also relates to a method for examining a subject by using the above method, a biomarker for disease detection, a method for using the biomarker for the disease detection, as well as a kit for the disease detection.