Exemplary embodiments of the present disclosure relate to providing an optical apparatus that is capable of acquiring representative Raman spec trams with high reliability from hyper spectrum images of samples. An optical apparatus according to this disclosure: receives a specifying input that specifies a specific Raman spectrum bandwidth as a reference signal; and extracts a Raman spectrum for a measurement point where an intensity of the reference signal is at or above a reference intensity.

An optical microscope according to one aspect of the present disclosure includes: a light source; a first scanner to scan a spot position of a light beam on a sample; an objective lens to focus the light beam deflected by the first scanner and cause the light beam to be made incident on the sample; a spectroscope including a slit on an incident side which an outgoing light emitted from an area on the sample onto which the light beam has been illuminated enters; a detector configured to detect an outgoing light from the spectroscope; and a first relay optical system including a first off-axis parabolic mirror that is arranged in an optical path from the first scanner to the objective lens and reflect the light beam deflected by the first scanner and a second off-axis parabolic mirror that reflects the light beam reflected in the first off-axis parabolic mirror.

Laser emission based microscope devices and methods of using such devices for detecting laser emissions from a tissue sample are provided. The scanning microscope has first and second reflection surfaces and a scanning cavity holding a stationary tissue sample with at least one fluorophore/lasing energy responsive species. At least a portion of the scanning cavity corresponds to a high quality factor (Q) Fabry-Prot resonator cavity. A lasing pump source directs energy at the scanning cavity while a detector receives and detects emissions generated by the fluorophore(s) or lasing energy responsive species. The second reflection surface and/or the lasing pump source are translatable with respect to the stationary tissue sample for generating a two-dimensional scan of the tissue sample. Methods for detecting multiplexed emissions or quantifying one or more biomarkers in a histological tissue sample, for example for detection and diagnosis of cancer, or other disorders/diseases are provided.

A structured illumination microscopy (SIM) system for generating single focal point patterns of a sample is disclosed. The SIM system performs a focusing, scaling and summing operation on each single focal point that completely scan the sample to produce a high resolution composite image.

A microscope for raster-free, confocal imaging of a sample arranged in a sample space has an illumination arrangement comprising a light source group having light sources which can be switched on individually, a detector arrangement, a pinhole arrangement which comprises a pinhole array and which has a plurality of pinhole elements which are adjacent to one another, wherein there is one pinhole element provided for each light source, and optics which irradiate each pinhole element with radiation of an individual light source of the light source group and confocally illuminate an individual spot located in the sample space, wherein one of the individual spots is associated with each pinhole element, and the individual spots are adjacent to one another in the sample space with respect to an incidence direction of the radiation, and the optics image the individual spots through the pinhole arrangement confocally on the detector arrangement.

Luminescence imaging apparatus, methods and computer program products are disclosed. A time-resolved luminescence imaging apparatus (100A) comprises: an optical assembly (2) operable to generate an array of beams; a scanner (4A) operable to scan the array of beams with respect to a sample (8), along a single scanning axis; and a detector assembly (10) having an array of detector elements, adjacent detector elements being spaced apart by an inter-element gap, each detector element being operable to detect emissions generated by the sample (8) in response to the array of beams. In this way, different locations on the sample (8) may be simultaneously scanned and imaged by the detector assembly (10) in order to image multiple parts of the sample (8) simultaneously. Also, by scanning along a single scanning axis, the complexity of the scanner (4A) is significantly reduced and the speed of scanning is increased compared to scanners which have to scan in two dimensions, such as a traditional raster scan mechanism.

Systems and methods for hyperspectral imaging are described. In one implementation, a hyperspectral imaging system includes a sample holder configured to hold a sample, an illumination system, and a detection system. The illumination system includes a light source configured to emit excitation light having one or more wavelengths and a diffractive element. The illumination system is configured to structure the excitation light into a predetermined two-dimensional pattern at a conjugate plane of a focal plane in the sample, spectrally disperse the structured excitation light in a first lateral direction, and illuminate the sample in an excitation pattern with the one or more wavelengths dispersed in the first lateral direction.

Embodiments of 3D imaging systems that use a multifunctional, nano structured metalens to replace the conventional microlens array in light field imaging are disclosed. The optical focusing properties of the metalenses provided by gradient metasurface optical elements. The gradient metasurfaces allow the properties of the elements of the metalens array to be changed by tuning the gradient metasurfaces.

Systems and methods for confocal imaging are described. In one implementation, a confocal imaging system may include a light source configured to emit excitation light having one or more wavelengths, a sample holder configured to hold a sample, a two-dimensional (2-D) imaging device, a first set of optical elements, and a second set of optical elements. The first set of optical elements may include a first spatial light modulator (SLM) and at least one lens. The first set of optical elements may together be configured to collimate the excitation light, apply a predetermined phase modulation pattern to the collimated excitation light, and illuminate the sample in an excitation pattern.

A functionally integrated laser scanning microscope for scanning a sample with laser illumination, selectably in a confocal, line or wide-field operating mode, comprising a laser light source, an illumination and detection beam path, a detection device and at least one objective, wherein the illumination and detection beam path has optical means for the configuration of the laser illumination, at least one scanner for scanning the sample with the laser illumination, and a beam splitter for separating illumination and detection light, and controllable optical elements for changing the beam guiding depending on the operating mode selected in each case.