A method is presented for obtaining an optically-sectioned image of a sample. The method comprises: providing an illumination beam through an imaging lens such that the illumination beam is focused at a focal plane of the imaging lens; obtaining a plurality of images of the sample. Obtaining comprises providing the illumination beam at a plurality of lateral positions on the focal plane and obtaining each image at each lateral position of the illumination beam, such that an intensity of the illumination beam on a portion of the sample at the focal plane varies for each of the plurality of lateral positions. The method further comprises detecting, using a detector, signals collected via the imaging lens; and constructing the optically-sectioned image based on the plurality of images. The constructing comprises: obtaining a plurality of signal values from the portion of the sample from the plurality of images; evaluating a threshold for the portion; and evaluating a pixel value by integrating a fraction of the plurality of signal values based on the threshold.

The present disclosure is directed to a method of disturbance correction and to a laser scanning microscope carrying out this method. Specifically, it is directed to an image recording method according to the MINFLUX principle, in which a spatially isolated fluorescence dye molecule is illuminated at a sequence of scan positions by an intensity distribution with a local intensity minimum, and the number of fluorescence photons emitted by the fluorescence dye molecule is detected at each of the scan positions. The location of the molecule is determined with a high spatial resolution from the scan positions and the numbers of fluorescence photons. A disturbance is captured when illuminating the fluorescence dye molecule and detecting the fluorescence light, said disturbance being considered in corrective fashion when determining the location of the fluorescence dye molecule.

In one example an apparatus can include a controller communicatively coupled to a droplet dispenser to deposit antibody fluid on a matrix of an immunoblotting array, the controller is to align the droplet dispenser with a protein band included in the matrix, instruct the droplet dispenser to deposit a first antibody fluid on to the protein band of the matrix, and instruct the droplet dispenser to deposit a second antibody fluid on to the protein band of the matrix, adjacent to the first antibody fluid.

A SPIM-microscope (Selective Plane Imaging Microscopy) and a method of operating the same having a y-direction illumination light source and a z-direction detection light camera. An x-scanner generates a sequential light sheet by scanning the illumination light beam in the x-direction. An electronic zoom is provided that is adapted to change the scanning length in the x-direction independently of a focal length of the illumination light beam and a size of the light sheet in the y-direction and in the z-direction, wherein the number of image pixels in x-direction is maintained unchanged by the electronic zoom independently of the scanning length in x-direction that has been selected.

The present invention relates to systems and methods for sequential operation of staining, imaging and sectioning of tissue samples by a processing system. After each layer of the sample is removed by the sectioning system, the system automatically stains the exposed surface of a sample to a depth to enable imaging of the remaining tissue. The system then repeats the sectioning, staining and imaging steps in sequence to image the sample.

The invention relates to a medical device (1) for the observation of a partly fluorescent object (2) such as tissue (3) comprising at least one fluorophore (4). The fluorophore (4) absorbs light in at least one spectral excitation waveband (46) and emits fluorescent light in at least one spectral emission waveband (54). In order to be able to observe also non-fluorescent regions in the tissue (3) without complicated filter arrangement, the medical device (1) according to the invention comprises at least one filter system (16, 38) which comprises, in a filter plane (18), comprises a filter area (20) and a transmission window (22). The filter area (20) comprises a band pass filter (24) having at least one passband (44) comprising the at least one excitation waveband. The transmission window has a passband (48) which is wider than the passband (44) of the filter area (20). In particular, a filter layer (64) of the filter area (20) may be missing in the transmission window (20).

Disclosed is a method for selecting a cancer treatment regimen for a subject.

Compact optical sources and methods for producing short and ultrashort optical pulses are described. A semiconductor laser or LED may be driven with a bipolar waveform to generate optical pulses with FWHM durations as short as approximately 85 ps having suppressed tail emission. The pulsed optical sources may be used for fluorescent lifetime analysis of biological samples and time-of-flight imaging, among other applications.

One non-limiting and exemplary embodiment provides a metal microscopic structure capable of detecting a low-concentration analyte with high sensitivity. The metal microscopic structure includes a base member including multiple protrusions arrayed at predetermined intervals, and multiple projections made of a metal film covering the base member and configured to generate surface plasmons upon irradiation with light. A film thickness of the metal film positioned in a bottom portion of a gap between every adjacent two of the multiple projections is greater than a height of the multiple protrusions and is more than or equal to 90% and less than or equal to 100% of a film thickness of the metal film deposited on top portions of the multiple protrusions.

An immunostaining method includes an irradiation process in which a specimen, which includes a target molecule including an electron donor, an antibody that is bound to the target molecule and that includes a generating agent for generating active species when irradiated with a first excitation light, and a pigment compound, is irradiated with the first excitation light; and in which the pigment compound and the electron donor are bound due to the active species generated from the generating agent when irradiated with the first excitation light.