Disclosed is a sample measurement device including a light applicator configured to apply a light to a sample so as to generate light from a particle in the sample; an optical block in which a plurality of prisms are fixed, each of the plurality of prisms including a light entry surface which allows entry thereinto of the light generated from the particle in the sample, a reflection surface configured to selectively reflect a part of the light having entered the light entry surface, and a light outputting surface configured to output the light reflected by the reflection surface; and a light receiver configured to receive the light outputted from the light outputting surface of each of the plurality of prisms.

An assay cartridge for detecting a target component in a fluid is provided. The assay cartridge including an optical element comprising: a light pathway comprising an input surface, reflective surface and output surface configured to enable light to enter, reflect and create an evanescent field in the vicinity of the reflective surface and exit the element; a plurality of capture components deposited on the reflective surface in the vicinity of the evanescent field; and a transmission surface configured to enable emissions from the evanescent field to exit the element; wherein the assay cartridge is a single use cartridge.

A system in an embodiment can comprise an optical assembly, an surface-plasmon-resonance (SPR) light source, and an SPR camera. The optical assembly can comprise a hemispherical prism comprising a top surface configured to support a SPR sensor; and a high numerical aperture (NA) lens located distal from the top surface of the hemispherical prism. The SPR light source can be configured to emit a light beam for SPR imaging. The SPR camera can be configured to capture an SPR image. The SPR sensor further can comprise a surface configured to contact a sample. The high NA lens can be configured to refract the light beam toward the hemispherical prism. The hemispherical prism can be configured to collimate the light beam, as refracted by the high NA lens, toward the SPR sensor. The high NA lens further can be configured to receive and refract the light beam toward the SPR camera, after the light beam is reflected by the surface of the SPR sensor. Other embodiments are disclosed.

Disclosed are optical trains for imaging systems. More particularly described are imaging systems configured to limit optical aberrations. Also disclosed are methods of limiting optical aberrations in imaging systems.

An apparatus and method of inline measurement of low-concentration hydrocarbons overlaps fluorescence, scatter and absorption spectroscopy devices so as to measure scatter and absorption of fluorescing oil and the excited fluorescence itself. The apparatus includes a fitting, an input port, an output port, and a sapphire tube having a hollow interior in fluid connection with the input port and the output port. Flow medium passes through the input port, the sapphire tube, and the output port. The apparatus also includes a light emitter, a first detector, and a second detector. The light emitter can include a lens, an absorption and scatter wavelength emitter, and a fluorescence wavelength emitter. An incident absorption and scatter beam and an incident fluorescence beam from the light emitter and parallel so as to determine free hydrocarbon, dissolved hydrocarbons, and solids in a sample within the sapphire tube.

We describe a super-resolution optical microscopy technique in which a sample is located on or adjacent to the planar surface of an aplanatic solid immersion lens and placed in a cryogenic environment.

A bio-detection device is provided. The bio-detection device includes a plurality of pixel units. Each of the pixel units includes a substrate, one or more pairs of reflective sub-polarizing units, and a plurality of reaction sites. The pairs of reflective sub-polarizing units are disposed on the substrate. The difference of the absolute value between respective polarizing angles of the reflective sub-polarizing units in each pair of reflective sub-polarizing units is 90°. The reaction sites are defined above the one or more pairs of reflective sub-polarizing units. The reaction sites and the reflective sub-polarizing units are in one-to-one correspondence.

A light sheet fluorescence microscopic imaging device for imaging transparentized droplets and a detection method are disclosed in the present application. The imaging device comprises a light source shaping module, a light sheet generation module, a sample control module, and an image capturing module. The light source shaping module is used to shape circular light into an elliptical light spot. The light sheet generation module is used to generate a sheet-like light beam according to the elliptical light spot. The sample control module is used to control a sample to move in a direction perpendicular to an optical axis when the sample is illuminated by the sheet-like light beam. The image capturing module is used to capture fluorescent signals excited in different positions when the sample is moving, so as to acquire a three-dimensional image sequence of the sample. The present application can be used to generate elliptical light within a short optical distance, so as to generate a high and thick light sheet, such that the shape of a light beam is more applicable to in-situ closed imaging of deep-layer droplets. At the same time, since no slit is required to block laser light, the energy utilization rate of the laser light is increased by more than four times, thereby improving the clear aperture in a large field of view, reducing the length of a capturing end, and resulting in a reduced volume and higher integration level.

Exemplary embodiments of the present disclosure include apparatus and methods to identify endometrial tissue.

Arrays of integrated analytical devices and their methods for production are provided. The arrays are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices allow the highly sensitive discrimination of optical signals using features such as spectra, amplitude, and time resolution, or combinations thereof. The devices include an integrated diffractive beam shaping element that provides for the spatial separation of light emitted from the optical reactions.