In an LC/MS/MS analysis: injecting a sample into a passage leading to a column group provided in a liquid chromatograph, the column group including a plurality of columns serially connected to each other and packed with different kinds of packing materials; supplying an eluant to one or a plurality of columns including a column located most downstream, to separate a portion of the target components in the sample in the one or plurality of columns, and sequentially elute those components from the most downstream column to perform mass spectrometry; and supplying a different eluant to one or a plurality of columns including the most downstream column, to separate at least a portion of the target components which stayed uneluted in the one or plurality of columns in the first analysis step, and sequentially elute those components from the most downstream column to perform mass spectrometry.

Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.

Apparatuses and associated methods are described for the efficient evaluation of C1-containing substrates, and especially for such evaluation conducted locally, or on-site, at a prospective facility for implementation of a biological conversion process for desired end product using a C1 carbon source. The exact composition of a given, industrial C1-containing substrate, as well as the range in composition fluctuations, are generally difficult to reproduce at a remote facility (e.g., a laboratory or a pilot-scale or demonstration-scale process), as required for the accurate prediction/modeling of commercial performance to justify large capital expenditures for commercial scale-up.

The invention provides an apparatus and system for the separation and optional analysis of the components of a sample of material, the apparatus and system comprising a cartridge comprising: at least one sample inlet port, at least one resin inlet port and a multiplicity of reagent and purge fluid input ports which are fluidically connected via a multiplicity of control valves to a multiplicity of chromatographic columns which are fluidically connected together in series; and a multiplicity of outlet ports wherein each outlet port additionally comprises an outlet valve which is adapted to control the flow of fluid through said outlet ports; wherein each of said multiplicity of chromatographic columns is aligned with one of said multiplicity of outlet ports so as to allow for fluid flow from said column through said outlet port. The system optionally additionally facilitates the analysis of the components. The invention additionally provides a method for the separation of the components of a sample of material which comprises the use of the apparatus and system of the invention. The apparatus, system and method of the invention are advantageously applied to the separation and analysis of radioactive materials.

Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.

Apparatus for processing a liquid comprising a target substance, preferably a biomolecule, is provided. The apparatus comprises at least a first and a second means for carrying out a unit operation, each means for carrying out a unit operation comprising a feed for a liquid in fluid connection with the inlet of a flow-controller comprising a variable flow inlet valve and an outlet, wherein at least one of the means for carrying out a unit operation comprises feeds for at least two liquids, the feeds being in fluid connection with the inlets of a multiple inlet flow-controller comprising two or more variable flow inlet valves for dosing the at least two liquids, the flow-controller also comprising an outlet; a feed for a liquid feedstock comprising the target substance in fluid connection with the outlet from the flow-controller thereby to enable combination of the feed for a liquid comprising the target substance with the mixed bioprocessing liquids to produce a device feed; a device for achieving a processing operation comprising a device inlet and a device outlet, the device inlet being in fluid connection with the device feed; and a means for imparting flow through the flow controller and from the feed for the liquid feedstock through the processing device via the device inlet; and wherein the feed for a liquid feedstock comprising the target substance for the second means for carrying out a unit operation comprises the outlet from the first means for carrying out a unit operation.

Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.

Provided are two-dimensional chromatography systems and methods for separating and/or analyzing complex mixtures of organic compounds. In particularly, a two-dimensional reversed-phase liquid chromatography (RPLC)supercritical fluid chromatography (SFC) system is described including a trapping column at the interface which collects the analytes eluted from the first dimension chromatography while letting the RPLC mobile phase pass through. The peaks of interest from the RPLC dimension column are effectively focused as sharp concentration pulses on the trapping column, which is subsequently injected onto the second dimension SFC column. The system can be used for simultaneous achiral and chiral analysis of pharmaceutical compounds. The first dimension RPLC separation provides the achiral purity result, and the second dimension SFC separation provides the chiral purity result (enantiomeric excess).

A method of performing a chromatographic separation includes modulating a portion of a flowstream to a trap column to retain at least one analyte from the flowstream on the trap column. A flow of a modifier is provided through the trap column to generate an elution comprising the at least one analyte. A flow of a compressible fluid-based chromatography (CFC) mobile phase or CFC solvent is merged with the elution from the trap column to generate a diluted elution. Carbon dioxide may be used as the CFC solvent or as a component of the CFC mobile phase. The diluted elution is provided to a CFC column where at least one analyte is focused at a head of the CFC column. Examples of a flowstream that may be used include an eluent from a chromatography column or a fluid flow from an extraction system.

A method of performing a chromatographic separation includes modulating a portion of a flowstream to a trap column to retain at least one analyte from the flowstream on the trap column. A flow of a modifier is provided through the trap column to generate an elution comprising the at least one analyte. A flow of a compressible fluid-based chromatography (CFC) mobile phase or CFC solvent is merged with the elution from the trap column to generate a diluted elution. Carbon dioxide may be used as the CFC solvent or as a component of the CFC mobile phase. The diluted elution is provided to a CFC column where at least one analyte is focused at a head of the CFC column. Examples of a flowstream that may be used include an eluent from a chromatography column or a fluid flow from an extraction system.