A sample separation device for separating a fluidic sample includes a fluid drive for driving a mobile phase and the fluidic sample when injected in the mobile phase, a sample separation unit for separating the fluidic sample in the mobile phase, and a control unit configured for bracketing the fluidic sample between two mobile phase portions of the mobile phase. At least one of the mobile phase portions is arranged directly next to the fluidic sample and has a higher solvent strength compared to a solvent of the fluidic sample.

Methods and systems to monitor and analysis unconventional reservoirs with wellbores with a substantially horizontal section. Monitoring and analysis is conducted in three dimensions using high-resolution geochemical fingerprinting analyses of rock samples and produced oil samples. The invention uses methods to preserve, prepare, extract, and/or analyze hydrocarbons in the pore spaces of or adsorbed in organic-rich rock samples, such as, but not limited to, drill cuttings and drill cores, using one or more combinations of physical energy sources, including, but not limited to, thermal, vapor pressure, and mechanical stress. The collected samples are transported and prepared in low temperature conditions, with parts of subsequent processing at very low temperatures, thereby allowing a fuller measurement of geochemical fingerprints for the extracted hydrocarbons using various analysis tools. More particularly, the treatment and process allows geochemical fingerprinting to very low carbon number ranges. The techniques of the present invention may be used to optimize well stacking and spacing, completion design, and cluster efficiency evaluation to improve unconventional reservoir economics.

A method for analyzing light n-alkane components and carbon isotopes in deep and ultra-deep source rocks includes: (S1) subjecting a 5A molecular sieve column to aging; (S2) pyrolyzing a source rock; and allowing a pyrolysis product to enter the 5A molecular sieve column; where n-alkanes are adsorbed and retained by the 5A molecular sieve column; allowing an outflow to pass through a fractionation plate and an empty column or a weak polarity column to be discharged; and (S3) performing programmed heating such that the n-alkanes adsorbed on the 5A molecular sieve column are successively desorbed according to molecular weight, and then pass through the fractionation plate and the HP-5 or DB-5 column to enter a mass spectrometer for composition analysis or isotopic analysis. An analysis system is further provided.

A system for analyzing a gas mixture, including at least one chromatography column, a mechanism injecting the mixture into the column, and a mechanism detecting compound(s) forming the gas mixture, the detection mechanism including at least one detector of nanosensor type of an outlet of the column and a detector of nanosensor type in the column, capable of detecting passage of the compounds. It is then possible to determine the velocity of each of the compounds within the system.

A rotary valve that includes a stator, a rotor and a plurality of sample channels. The stator includes a stator surface having an inlet port, an outlet port and a plurality of selectable ports. The rotor includes a rotor surface having a first rotor channel and a second rotor channel. The rotor is configurable in a plurality of rotor positions, each of which couples the inlet port to one of the selectable ports through the first rotor channel and couples the outlet port to another one of the selectable ports through the second rotor channel. The two selectable ports are coupled to each other through one of the sample channels. The rotor has a bypass state defined by a rotor position, or angular range of rotor positions, at which the inlet port is coupled to the outlet port through the second rotor channel.

The EM algorithm for a Gaussian mixture model is applied to the separation of peaks that overlap one another on a chromatogram. However, the number of overlapping components is unknown. Thus, a suitable number of models is set, and the fitting of model parameters is performed while an actually measured signal is appropriately divided for each model by the EM algorithm. Then, when a solution converges, a determination is made as to whether a peak-like waveform is present in a residue signal that is not divided. When the peak-like waveform is present, a peak model is added. The EM algorithm is executed again. In the M step, optimization is performed using, not only a simple Gaussian function, but also a modified Gaussian function assuming a tailing. In the M step, the estimation of a spectrum assuming a chromatogram and the estimation of a chromatogram assuming a spectrum are repeatedly performed.

Described are a chromatographic separation device and a method for performing a chromatographic separation. The device two chromatographic separation modules in serial communication. The first module is adapted to receive a gradient includes mobile phase. The second module receives the gradient mobile phase that exits from the first module. The first and second modules include chromatographic sorbents that differ in one or more of composition, particle size and sorbent temperature. The retentivity of the second module is greater than the retentivity of the first module and the chromatographic dispersion of the second module is less than the chromatographic dispersion of the first module. The width of a chromatographic peak eluted from the first module is greater than a width of the same chromatographic peak after elution from the second module. The device has a high peak capacity without the need to pack a full column length with small sorbent particles.

Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.

The present invention relates to compositions comprising erenumab and one or more erenumab variants, including isomerization variants, deamidation variants, acidic variants, and HMW species. Pharmaceutical formulations comprising the erenumab compositions and methods of using and characterizing the compositions are also described.

Disclosed are non-contiguous sample fractionating and concatenating device and a dual online multidimensional liquid chromatography system having the same. The non-contiguous sample fractionating and concatenating device according to an embodiment of the present disclosure includes a sample supply module which supplies a sample to be analyzed, and a sample fractionation module connected to the sample supply module, and which is continuously supplied with the sample, sets a plurality of unit sample supply times obtained by equally dividing a total sample supply time during which the sample is supplied from the sample supply module, sets a plurality of unit fractionation intervals obtained by equally dividing each of the plurality of unit sample supply times, and concatenates and stores the sample supplied during corresponding unit fractionation intervals within each unit sample supply time to acquire a plurality of fractions.