Sperm can be separated by nonlethal methods in a sorting track that into cubicles having different cubicle characteristics. A test sample of sperm from selected cubicles can be sacrificed to determine their prevalent genetic characteristics. The visually identifiable characteristics of sperm from the test samples can also be correlated with prevalent genetic characteristics. A living sperm, sharing selected genetic characteristics, can be selected from remaining sperm having similar identifiable characteristics in a respective cubicle and used for fertilizing an egg.

The present invention relates to a composition for use in low dose artificial insemination and to a process for artificial insemination of a female mammal using a dose of sperm originating from a male mammal of the same species that is significantly lower than necessary in the absence of the composition. The preferred female mammal is a sow (Sus scrofa), and preferably the composition is for use in artificial uterine insemination, and the process for artificial insemination preferably is uterine insemination.

The present invention relates to a composition for use in low dose artificial insemination and to a process for artificial insemination of a female mammal using a dose of sperm originating from a male mammal of the same species that is significantly lower than necessary in the absence of the composition. The preferred female mammal is a sow (Sus scrofa), and preferably the composition is for use in artificial uterine insemination, and the process for artificial insemination preferably is uterine insemination.

The present invention relates to a composition for use in low dose artificial insemination and to a process for artificial insemination of a female mammal using a dose of sperm originating from a male mammal of the same species that is significantly lower than necessary in the absence of the composition. The preferred female mammal is a sow (Sus scrofa), and preferably the composition is for use in artificial uterine insemination, and the process for artificial insemination preferably is uterine insemination.

Provided are stem cells-induced Serotoli-like cells, methods of preparing the same, and uses thereof. Sertoli-like cells according to one embodiment can be differentiated from embryonic stem cells with excellent proliferative capacity, and thus, can be obtained in large quantities. Also, since the Sertoli-like cells secrete immunosuppressive substances and form immune privilege and induce anti-inflammatory functions, they can be used for development of the cell therapeutic agent.

The present invention refers to field of assisted reproductive technologies, in particular to a sperm storage media, and describes mTOR enhancers, preferably the mTOR activator MHY 1485, as supplement for storage media, said supplemented media increasing sperm motility and viability during short-term room temperature storage, while sustaining the metabolic rates, without changing its fertilization potential (capacitation) and DNA integrity.

The invention relates to methods of increasing genetic merit of swine by establishing a plurality of mating subtypes for a line of swine, and determining a percentage of progeny that are male for each of the mating subtypes, or a percentage of progeny that are female for each of the mating subtypes, that would result, relative to a control, in an increase in genetic merit in the line; the invention further relates to sorting a sperm cell sample from a male swine in one of the mating subtypes into one or more subpopulations of sperm cells, wherein a majority of sperm cells in a subpopulation of sperm cells bear X chromosomes or Y chromosomes, and inseminating one or more female swine in the one of the mating subtypes with the subpopulation of sperm cells to achieve the percentage of progeny that are male, or the percentage of progeny that are female, determined to increase genetic merit relative to the control.

The invention relates to methods of increasing genetic merit of swine by establishing a plurality of mating subtypes for a line of swine, and determining a percentage of progeny that are male for each of the mating subtypes, or a percentage of progeny that are female for each of the mating subtypes, that would result, relative to a control, in an increase in genetic merit in the line; the invention further relates to sorting a sperm cell sample from a male swine in one of the mating subtypes into one or more subpopulations of sperm cells, wherein a majority of sperm cells in a subpopulation of sperm cells bear X chromosomes or Y chromosomes, and inseminating one or more female swine in the one of the mating subtypes with the subpopulation of sperm cells to achieve the percentage of progeny that are male, or the percentage of progeny that are female, determined to increase genetic merit relative to the control.

Provided are methods of in vitro maturation of human spermatogonium, comprising culturing the spermatogonium in a three-dimensional methylcellose culture system (MCS) under conditions capable of differentiating said human spermatogonium into an elongated spermatid, thereby in vitro maturing the human spermatogonium. Also provided is an in vitro matured sperm obtainable according to the method of the invention and methods of treating subjects in need of mature sperm cells.

The invention provides, inter alia, methods of improving sperm function and related methods of fertilization, together with preparations of activated or potentiated sperm. The invention additionally provides articles of manufacture suitable for performing the methods provided by the invention. The methods provided by the invention, in certain embodiments entail energy depletion with subsequent staged reintroduction of different energy sources.