The present disclosure relates to a three-dimensional, degradable medical implant for regeneration of soft tissue comprising a plurality of volume-building components and a mesh component which is substantially made of monofilament or multifilament fibers, wherein each volume-building component is attached to at least one point on a surface of the mesh component, and wherein the projected surface area of each volume-building component, when projected on the surface of the mesh component, corresponds to a maximum of one tenth of the surface area of the mesh component.

Provided is a method for producing a three-dimensional tissue having a vascular system structure, said method comprising: (a) a step for forming a vascular system structure template using a gel; (b) a step for forming a three-dimensional tissue in the vicinity of the template; (c) a step for dissolving the template using a cationic solution; and (d) a step for seeding vascular endothelial cells and/or lymphatic vessel endothelial cells in a void remaining after the dissolution of the template. Also provided is a method for producing a three-dimensional tissue having a vascular system structure, said method comprising: (i) a step for forming a vascular system structure template using a gel; (ii) a step for seeding vascular endothelial cells and/or lymphatic vessel endothelial cells on the template; (iii) a step for forming a three-dimensional tissue in the vicinity of the cells seeded above; and (iv) a step for dissolving the template using a cationic solution. Also provided is a three-dimensional tissue comprising a gel which has a vascular system structure.

Provided is an alveolar bone augmentation scaffold system. The scaffold system includes the following structures: a porous augmentation scaffold fabricated by 3D printing using composite materials for filling alveolar bone defects; a mechanical separating plate, wrapped around the porous augmentation scaffold with a biomimetic structure for restoring alveolar bone defects; the augmentation scaffold is provided with a first region close to dental pulp, a second region away from the dental pulp, and a third region surrounding the second region, wherein pore diameters of three-dimensional porous structure of the above three regions are R1, R2, and R3 respectively, and they satisfy R1≥R2>R3.

Disclosed herein are compositions of biodegradable scaffolds combined with mesenchymal stem cells and methods of use thereof for the regeneration of cranial sutures and treatment of craniosynostosis, which can help reverse increased intracranial pressure and skull and neurocognitive abnormalities.

The disclosure provides bone graft materials, methods for their use and manufacture. Exemplary bone graft materials comprise combining a radiopaque component with a cancellous bone component to produce a bone graft material, wherein the cancellous bone component comprises native osteoreparative cells. Methods for treating a subject with the bone graft material are also provided.

A system and method for tissue fabrication involves the use of charge manipulation between two biomaterials to generate a shrinking response, which effectively enhances the resolution of bioprinted hydrogels. The charge manipulation can be utilized to generate tissue engineered thin, membranous tissues, such as the periosteum, which is approximately one hundred microns in thickness. Thin membranous tissues in the body also have relatively complex anatomies containing multiple cell populations, and no prior strategies allow for the effective and biomimetic generation of these tissues, which can have significant impact on tissue regeneration.

A bone gel composition consists of cortical bone. The cortical bone is made from cut pieces freeze-dried then ground into particles and demineralized then freeze-dried. A volume of the particles is placed in a solution of sterile water to create a mixture, the water volume being at least twice the particle volume, the mixture is autoclaved under heat and pressure to form a gelatin, the resulting bone gel is formed into sheets having a thickness (t).

The present invention relates to methacryloyl-gelatin and acryloyl-gelatin with low pyrogenic activity, in particular with low lipopolysaccharide content. The (meth)acryloyl-gelatin is further characterized by low (meth)acrylic acid content. The invention further relates to methods for preparing said (meth)acryloyl-gelatin, which do not require a dialysis step. The invention further relates to hydrogels comprising this (meth)acryloyl-gelatin, as well as uses thereof for tissue-engineering applications, and as bio-ink or bio-resin.

A system and method for printing cells in a medium. A multi-dimensional printer, stably constructed of low-mass parts, can include a computer numerically controlled system that can enable motors driving delivery systems. The motors can include encoders that can enable achieving arbitrary resolution. The motors can drive ballscrews to enable linear motion of delivery systems, and the delivery systems can enable printing of a biological material in a pre-selected pattern in a petri dish. The petri dish can accommodate a medium such as a gel, and can further accommodate a vision system that can detect actual position and deflection of the delivery system needle. The printer can accommodate multiple delivery systems and therefore multiple needles of various sizes.

Biodegradable antimicrobial films are provided that are solid at room temperature and substantially liquefy in situ after implantation into a mammal, such as a human patient. Methods of using the films to cover a medical device, such as a breast implant, prior to insertion into a subject are also provided.