Techniques, mixtures, mixing and delivery kits, and improved delivery instruments for implantation of micronized allograft tissue over a microfractured defect. Allograft cartilage tissue is delivered over a cartilage defect that has been debrided and microfractured, without the need for a periosteal covering or separate type of patch sewn over the top. The allograft tissue may be any micronized cartilage particulates obtained by various methods, for example, cartilage delivered in its native form, dehydrated via lyophilization, freeze-dried, dehydrated via desiccation, or dehydrated by any other method.

A process for the preparation of chondrocyte cell suspension and its use in defect site of knee or ankle or shoulder or wrist or elbow or hip of subject.

Methods for developing a decellularized tissue and biomaterials for use as biomimetic grafts or in vitro cellular scaffolds formed with the decellularized tissue are described. The biomaterials are particularly well suited for use as an intervertebral disc graft. The decellularized tissue is formed from an intervertebral disc source tissue and can be substantially decellularized and substantially free of potential immunogenic material (e.g., DNA and RNA), while maintaining ECM materials including both glycosaminoglycan and collagen.

The present invention aims to provide a method for producing a cartilage tissue, which enables production of a cartilage tissue having an appropriate thickness, form, and mechanical strength, and a cartilage tissue produced by the method for producing a cartilage tissue. Provided is a method for producing a cartilage tissue including a step of seeding a collagenase-treated cartilage tissue piece in the form of a block 50 to 1,000 m on a side onto a porous substrate composed of a bioabsorbable material.

The present disclosure provides tissue fillers. The tissue fillers can include a plurality of tissue particles formed from acellular tissue matrix fragments. The tissue fillers can be used to fill tissue sites, such as voids formed after tissue resection.

Provided are compositions and methods for achieving time-delayed cross-linking of hyaluronic acid (HA) to be used as a soft tissue filler. The compositions may include at least one component that facilitates cross-linking of HA, which is encapsulated and released in a time-delayed manner. Also provided are methods for treating or augmenting tissue using such compositions.

An object is to provide a technique that can evaluate biological tissues such as cartilage tissue and regenerated tissues such as regenerated cartilage. A method for observing a dynamic physical property of a biological tissue according to the present invention is that a biological tissue is irradiated with a pulsed light having a wavelength of a far-infrared wavelength region modulated into circular polarized lights by applying bias voltages to a radiation means (3) having an antenna electrode films of orthogonal (2)-axis structure with phases shifted using high-voltage high-speed modulation means (13), and dynamic physical property of the biological tissue is observed on the basis of a spectrum obtained by vibration optical activity spectroscopy.

The present invention provides a cartilage matrix having a high decellularization level arid a low glycosaminoglycan (GAG) content. The cartilage matrix exhibits desirable characteristics, for example, cohesiveness, tackiness and malleability, for use in cartilage repair. Also provided is a method of preparing the cartilage matrix, comprising decellularizing a cartilage to generate a decellularized cartilage, and deglycosylating the decellularized cartilage.

A method for making a porous devitalised scaffold suitable for use in repair of osseous, chondral, or osteochondral defects in a mammal comprises the steps of providing micronized extracellular matrix (ECM) tissue, mixing the micronized extracellular matrix with a liquid to provide a slurry, and freeze-drying the slurry to provide the porous scaffold. A porous scaffold suitable for use in repair of osseous, chondral, or osteochondral defects in a mammal and comprising a porous freeze-dried matrix formed from micronised decellularised extracellular matrix tissue is also described.

A method for producing a decellularized tissue includes steps of lysing a cell of a biological tissue using a liquid containing a liquefied gas, and degrading a nucleic acid component contained in the lysed cell of the biological tissue using a nucleolytic enzyme.