The present disclosure is drawn to compositions and methods of making and using lyophilized platelet lysates. Specifically, a method of preparing a composition suitable for therapeutic use or as a culture medium can comprise steps of concentrating platelets from a platelet source to form a platelet rich portion of the platelet source, and lysing the platelets in the platelet rich portion to form a plurality of lysates. An additional step includes lyophilizing the lysates to form lyophilized platelet lysates in a composition with released concentrations of available growth factors, cytokines, and chemokines. In one example, at 30%, by platelet count, of platelets from a platelet source can be lysed using this process.

The present disclosure features adhesive compositions and methods of use thereof related to the medical, veterinary, and dental fields.

The present disclosure relates to methods of preparing personalized blood vessels, useful for transplantation with improved host compatibility and reduced susceptibility to thrombosis. Also provided are personalized blood vessels produced by the methods and use thereof in surgery.

In some forms, the present disclosure provides systems and methods for isolation of platelet rich plasma from a liquid tissue sample. In accordance with some forms of the disclosed systems and methods, provide rapid separation of blood components with the need for centrifugation. Accordingly, in one embodiment, the present disclosure provides a method for isolating platelet rich plasma, the method comprising combining a liquid tissue sample with a polymer composition, the polymer composition comprising polyethylene glycol.

The present invention concerns a tissue-engineered medical device, as well as a method for the production said medical device, comprising the following steps: providing a polymer scaffold comprising a mesh comprising polyglycolic acid, and a coating comprising poly-4-hydroxybutyrate; application of a cell suspension containing preferably human cells to the polymer scaffold; placement of the seeded polymer scaffold in a bioreactor and mechanical stimulation by exposure to a pulsatile flux of incremental intensity, thereby forming an extracellular matrix; mounting of the graft on a conduit stabilizer and incubation in cell culture medium; decellularisation of the graft in a washing solution; nuclease treatment of the graft; and rinsing of graft. The invention further comprises and various steps of quality control of the tissue-engineered medical device.

The disclosure provides a use of hydrogel composition for alleviating degenerative joint and tendon tear. The hydrogel composition includes 100 parts by weight of therapeutic agent and 120-380 parts by weight of biodegradable copolymer, wherein the therapeutic agent comprises platelet-rich plasma (PRP), doxorubicin, transforming growth factor, bovine serum albumin, or a combination thereof. The biodegradable copolymer has a structure of Formula (I) or Formula (II):

##STR00001## wherein A is a hydrophilic polyethylene glycol polymer; B is a hydrophobic polyester polymer; BOX is a bifunctional group monomer of 2, 2-bis(2-oxazoline) used for coupling the blocks A-B or B-A-B; and n is 0 or an integer greater than 0.

The present invention relates to a composition and a kit for regeneration and treatment of cartilage, preferably, fibrous cartilage or elastic cartilage and a regeneration method using the same. A composition and a kit for cartilage regeneration according to the present invention may be administered simply in a minimally invasive manner to a site in which fibrous cartilage or elastic cartilage is needed to be regenerated or restored from injury, exhibit resistance to degradation enzymes without toxicity within the body, and are attached to or detained at and thus retained at the injured, administered site, whereby behavioral improvement may be brought about in surrounding cells, which leads to effectively inducing defected tissues of the meniscus to be regenerated. Therefore, the composition of the present invention is useful as a mediator for aiding the regeneration of biological tissue defected regions in the biomaterials field.

The present invention relates to an Autologous Bone Graft Substitute composition (ABGS) for inducing new bone formation, promoting bone growth and treating of bone defect, a preparation method of the Autologous Bone Graft Substitute composition, and a kit for preparing the Autologous Bone Graft Substitute composition of implant. In a particular aspect, the invention relates to an injectable/extrudable/implantable Autologous Bone Graft Substitute composition for use in treatment of bone defects, inducing new bone formation and promoting bone growth for bone fracture healing, spinal fusions and to repair bone defects in bone reconstructive procedures of orthopedic and oral maxillofacial-dental surgeries.

Embodiments of the invention include instruments and methods useful in preparing and delivering graft material to a surgical site. Some embodiments may particularly be directed to forming a graft from a blood clot and accurately and effectively handling and delivering the graft to a surgical site. Graft material may include blood components such as clotted fibrin derived from a patient's or a donor's blood.

A device for mixing a bone material with a liquid is provided. The device comprises a chamber having a proximal end and a distal end, and the bone material disposed within the chamber, the bone material comprising a DBM pellet of milled and lyophilized demineralized bone fibers; and a plunger having at least a portion slidably disposed within the proximal end of the chamber and configured to dispense the bone material mixed with liquid from the distal end of the chamber, when the plunger is in an extended position.