Methods of treating a complex wound by administering to a complex wound in the individual a therapeutically effective amount of a fetal support tissue product to treat the complex wound. Methods of treating a complex lower extremity ulcer by administering to a complex lower extremity ulcer in the individual a therapeutically effective amount of a fetal support tissue product to treat the complex lower extremity ulcer. Methods of reducing or preventing scar formation from granulation tissue by administering a fetal support tissue product to granulation tissue. Methods of repairing a spina bifida defect by administering to the defect in the individual a therapeutically effective amount of an umbilical cord product.

An anchor is anchorable to tissue of a ventricle downstream of a heart valve of a subject. Each wing of a pair of wings defines a lateral surface and a medial surface, such that the medial surface of one wing of the pair faces the medial surface of the other wing of the pair. The wings are coupled to the anchor such that, when the anchor is anchored to the tissue, the anchor supports the wings at the valve, with the lateral surface of each wing facing a respective leaflet of the valve. During systole, the lateral surface of each wing is in contact with the respective leaflet, and the medial surfaces of the wings move into contact with each other, obstructing retrograde blood flow. During diastole, the medial surfaces move out of contact with each other, facilitating antegrade blood flow. Other implementations are also described.

Described herein are compositions comprising decellularized extracellular matrix derived from skeletal muscle or other suitable tissue, and therapeutic uses thereof. Methods for treating, repairing or regenerating defective, diseased, damage, ischemic, ulcer cells, tissues or organs in a subject preferably a human, with diseases associated with muscular degeneration, using a decellularized extracellular matrix of the invention are provided. Methods of preparing culture surfaces and culturing cells with absorbed decellularized extracellular matrix are provided.

Described herein are compositions comprising decellularized extracellular matrix derived from skeletal muscle or other suitable tissue, and therapeutic uses thereof. Methods for treating, repairing or regenerating defective, diseased, damage, ischemic, ulcer cells, tissues or organs in a subject preferably a human, with diseases associated with muscular degeneration, using a decellularized extracellular matrix of the invention are provided. Methods of preparing culture surfaces and culturing cells with absorbed decellularized extracellular matrix are provided.

Tissue compositions and methods of preparation thereof are provided. The tissue compositions can be used to treat or regenerate muscle tissue. The compositions can be configured to provide increased strength compared to other muscle matrices.

The present invention provides methods of regenerating muscle tissue and methods of treating volumetric muscle loss comprising administering decellularized fetal matrix scaffold. Also provided are methods for treating soft tissue injury and improving reconstructive surgery.

A method for treating damaged or diseased mammalian tissue that includes the steps of (i) providing a sheet structure formed from acellular dermal mammalian tissue that includes extracellular matrix (ECM) and a supplemental bioactive component, such as a nucleic acid, and/or a cell, and (ii) delivering the sheet structure to the damaged or diseased mammalian tissue, wherein the sheet structure induces angiogenesis and, thereby, regeneration of new mammalian tissue.

A composition for regeneration of skeletal muscle includes a nerve cell secretome or an isolate thereof. An implantable therapeutic material for regeneration of skeletal muscle includes a hydrogel and nerve cells encapsulated within the hydrogel, wherein at least some of the nerve cells are living. A method of regenerating skeletal muscle includes applying the composition or implanting the implantable material adjacent to skeletal muscle myoblasts and/or myogenic cells to regenerate skeletal muscle therefrom.

Disclosed herein are contractile cell constructs, methods for using them to treat disease, and methods for making them.

The present disclosure describes methods of treating an injury in a subject using placental tissue streamers, engineered tissue placental tissue hybrids, suture placental tissue hybrids, placental tissue patch hybrids, and tissue hybrids, and the use of these compositions to repair, treat, or support an injury or degenerative process in a subject.