A method is provided for preparing an ECM material, including an ECM gel, from regenerative or regenerating tissue. ECM material prepared from regenerative or regenerating materials also is provided.

The growth factor profile, connective tissue matrix constituents, and immunoprivileged status of urodele extracellular matrix (ECM) and accompanying cutaneous tissue, plus the presence of antimicrobial peptides there, render urodele-derived tissue an ideal source for biological scaffolds for xenotransplantation. In particular, a biological scaffold biomaterial can be obtained by a process that entails (A) obtaining a tissue sample from a urodele, where the tissue comprises ECM, inclusive of the basement membrane, and (B) subjecting the tissue sample to a decellularization process that maintains the structural and functional integrity of the extracellular matrix, by virtue of retaining its fibrous and on-fibrous proteins, glycoaminoglycans (GAGs) and proteoglycans, while removing sufficient cellular components of the sample to reduce or eliminate antigenicity and immunogenicity for xenograft purposes. The resultant urodele-derived biomaterial can be used to enhance restoration of skin homeostasis, to reduce the severity, durations and associated damage caused by post-surgical inflammation, and to promote progression of natural healing and regeneration processes. In addition, the biomaterial promotes the formation of remodeled tissue that is comparable in quality, function, and compliance to undamaged human tissue.

The invention concerns the treatment of bioprosthetic tissues a Cyclodextrin, preferably in association with Ethanol.

The present invention discloses a braided silk scaffold with adjustable mechanical and degradation properties, and a preparation method and use thereof, belonging to the field of three-dimensional scaffold materials for tendon/ligament repair. The preparation method includes braiding at least one silk strand to form a silk core; placing 1-6 bundles of silk cores in a braiding machine, and braiding at least one layer of silk cladding on the surface of the silk cores to form a silk base frame; removing sericin from the silk base frame; soaking the silk base frame in a collagen solution with a concentration of 3-20 mg/ml, and cross-linking the silk base frame in a vacuum thermal cross-linking machine to obtain the silk scaffold. The braided silk scaffold with adjustable mechanical and degradation properties according to the present invention has good mechanical properties and biocompatibility.

The present disclosure provides improved biomaterials extracted from fibrous meniscal cartilage (FMC). The materials are at least partially decellularized and enzyme treated to remove at least a portion of the elastin and blood vessels found in FMC. Such biomaterials can be employed as tissue scaffolds, such as in transplant procedures.

Biomaterials for tissue regeneration and engineering applications and methods of making and use thereof are described, as well as constructs and kits derived from the biomaterials. The biomaterials can be derived from extracellular matrix and functionalized to make them crosslinkable and amenable to tuning of their material properties.

Compositions including a first soft tissue-derived matrix and a second soft tissue-derived matrix are provided, as well as methods of making such compositions. In some embodiments, the composition comprises delipidated, decellularized adipose tissue-derived matrix and delipidated, decellularized fascial tissue-derived matrix, which may be combined in various proportions. Such adipose-fascia matrix compositions provide improved volume retention when implanted into a patient. The composition may further include exogenous cells or other substances, and/or a carrier. The composition is suitable for use in plastic surgery procedures, including reconstructive or cosmetic surgery procedures, as well as procedures for wound treatment and tissue regeneration. The methods for making the compositions may involve separation of first and second soft tissues from one another, followed by performing one or more treatments on the separated soft tissues, then combining the treated soft tissues and, optionally, performing one or more additional treatments on the combined soft tissues.

Tissue processing techniques are described involving association of tissue with a form under pressure to more precisely adapt the crosslinked tissue to the form. Pressure can be applied through holding of the issue on a porous form with suction on the form maintain tight adherence of the tissue on the form. In some embodiments, the tissue on the form is placed with the crosslinking solution in a bag that is then vacuum sealed to have the evacuated bag hold the tissue on the form. Whether or not the tissue is crosslinked on a form, glutaraldehyde can be used for crosslinking in a substantially unpolymerized state to achieve distinct crosslinked tissue properties.

A system and method for tissue fabrication involves the use of charge manipulation between two biomaterials to generate a shrinking response, which effectively enhances the resolution of bioprinted hydrogels. The charge manipulation can be utilized to generate tissue engineered thin, membranous tissues, such as the periosteum, which is approximately one hundred microns in thickness. Thin membranous tissues in the body also have relatively complex anatomies containing multiple cell populations, and no prior strategies allow for the effective and biomimetic generation of these tissues, which can have significant impact on tissue regeneration.

Methods for decellularizing organs and tissues in vitro and in vivo are provided, as are methods of maintaining organ and tissue frameworks and methods of recellularizing organs and tissues, thereby providing an approach to needed organs or tissues.