The invention relates to peptides including DEDE(SSD).sub.nDEG indicated by SEQ NO. 1, RRRDEDE(SSD).sub.nDEG indicated by SEQ NO. 2, RRRGDEDE(SSD).sub.nDEG indicated by SEQ NO. 3, and LKKLKKLDEDE(SSD)nDEG indicated by SEQ NO. 4, wherein n is an integer from 2 to 20. The invention also relates to phosphorylating these peptides at multiple amino acid sites by employing casein kinases. These phosphorylated peptides may be used in various applications such as forming mineralized collagen fibrils and biomimetic composites for use in tissue repair and regeneration.

The invention relates to peptides including DEDE(SSD).sub.nDEG indicated by SEQ NO. 1, RRRDEDE(SSD).sub.nDEG indicated by SEQ NO. 2, RRRGDEDE(SSD).sub.nDEG indicated by SEQ NO. 3, and LKKLKKLDEDE(SSD)nDEG indicated by SEQ NO. 4, wherein n is an integer from 2 to 20. The invention also relates to phosphorylating these peptides at multiple amino acid sites by employing casein kinases. These phosphorylated peptides may be used in various applications such as forming mineralized collagen fibrils and biomimetic composites for use in tissue repair and regeneration.

The present application relates to amphiphilic polypeptide materials, methods for making and using the same. Provided amphiphilic polypeptide materials are water-based and manufactured using all aqueous processing. Provided materials exhibit a capacity to encapsulate and store biologically active molecules or macromolecules. Such biologically active molecules or macromolecules retain their structure and the biological activity so that these biologically active molecules or macromolecules are not materially degraded, reduced, and/or inhibited by processing steps or exposure. Provided materials possess unique mechanical and structural properties, including size, density, moldability and machinability.

The present application relates to amphiphilic polypeptide materials, methods for making and using the same. Provided amphiphilic polypeptide materials are water-based and manufactured using all aqueous processing. Provided materials exhibit a capacity to encapsulate and store biologically active molecules or macromolecules. Such biologically active molecules or macromolecules retain their structure and the biological activity so that these biologically active molecules or macromolecules are not materially degraded, reduced, and/or inhibited by processing steps or exposure. Provided materials possess unique mechanical and structural properties, including size, density, moldability and machinability.

The disclosure is directed towards polypeptide substrates and methods of synthesis thereof. Such substrates can be embedded with calcium ions from a number of ionic sources. These calcium-embedded, polypeptide substrates can be used to grow a variety of crystal structures including flower-shaped, onion-shaped, and needle-like crystal structures. As such, the disclosure is additionally directed towards methods of crystal growth from polypeptide substrates. Compositions of the disclosure can be used in a wide variety of medical and other applications.

A biphasic osteotendinous repair scaffold is disclosed. The biphasic osteotendinous repair scaffold comprises a first phase comprising electrochemically aligned collagen (ELAC) threads and a calcium phosphate mineral and having a major surface. The biphasic osteotendinous repair scaffold also comprises a second phase comprising ELAC threads and having a major surface. The ELAC threads of the first phase are braided and crosslinked. The ELAC threads of the second phase are braided and crosslinked. The ELAC threads of the first and second phases form first and second interconnected macroporosities throughout the first and second phases, respectively. The calcium phosphate mineral of the first phase is distributed on the major surface of the first phase and within the interconnected macroporosity of the first phase. The second phase is adjacent to the first phase.

A biphasic osteotendinous repair scaffold is disclosed. The biphasic osteotendinous repair scaffold comprises a first phase comprising electrochemically aligned collagen (ELAC) threads and a calcium phosphate mineral and having a major surface. The biphasic osteotendinous repair scaffold also comprises a second phase comprising ELAC threads and having a major surface. The ELAC threads of the first phase are braided and crosslinked. The ELAC threads of the second phase are braided and crosslinked. The ELAC threads of the first and second phases form first and second interconnected macroporosities throughout the first and second phases, respectively. The calcium phosphate mineral of the first phase is distributed on the major surface of the first phase and within the interconnected macroporosity of the first phase. The second phase is adjacent to the first phase.

A hybrid composite and method for producing a polymer network are provided. The hybrid composite includes nanocrystalline hydroxyapatite (nHA) and polyurethane. The method for producing a polymer network includes reacting nanocrystalline hydroxyapatite (nHA) particles with lysine derived triisocyanate (LTI) to form a nHA/LTI hybrid prepolymer and reacting the prepolymer with a thioketal (TK) diol to form a nHA/poly(thioketal urethane) (PTKUR) hybrid polymer network.

A hybrid composite and method for producing a polymer network are provided. The hybrid composite includes nanocrystalline hydroxyapatite (nHA) and polyurethane. The method for producing a polymer network includes reacting nanocrystalline hydroxyapatite (nHA) particles with lysine derived triisocyanate (LTI) to form a nHA/LTI hybrid prepolymer and reacting the prepolymer with a thioketal (TK) diol to form a nHA/poly(thioketal urethane) (PTKUR) hybrid polymer network.

The present invention discloses a highly stretchable matrix, comprising mesh of lattice structures of microfibrillar filaments, having pore size enlargeable up to 8 times by moving the microfibrillar filaments perpendicular to the longitudinal axis of the microfibrillar filaments without losing its integrity. The invention also pertains to a method of preparing said highly stretchable matrix comprising the steps of: electrostatic spinning of the polymeric solution as microfibers, creating an air-flow at the inter-phase of microfibers to completely eliminate the solvent from the surface of the microfibers bundles to avoid inter-fibrillar bonding after collection and dispersing the microfibers in a direction perpendicular to the longitudinal axis of the fibers, 6-12 times the original width using a dispersion unit to obtain a stretch responsive fibrillar matrix.