Devices, systems, and methods for depleting fluids of immunoglobulins and leukocytes are disclosed.

The disclosure provides for a container system and method for the cryopreservation and resuspension of a body fluid. The container system may include a cryopreservation container comprising the body fluid in a cryopreservation liquid, a resuspension container comprising a resuspension solution, a connection tube sterilely connecting the cryopreservation container and the resuspension container, and at least one shut-off element actively associated with the connection tube.

A centrifugal separation system includes a blood bag system and a centrifugal separation and transferring device. The blood bag system is equipped with a single clamp that is mounted over a second tube and a third tube. In a first blocking portion of a first insertion hole of the clamp, a portion thereof except for a portion connected with a first opening portion is closed by a main body. On the other hand, a second insertion hole of the clamp includes a tube detaching portion, that is connected with a second blocking portion and that enables the third tube to be detached from the main body.

A blood bag system includes one clamp mounted over a second tube and a third tube. In a first insertion hole of a main body of the clamp, a portion of a first blocking portion excluding a portion connected with a first opening portion is closed by the main body. The main body can be deformed from a first state to a second state. In the first state, the third tube having been moved into a second blocking portion of a second insertion hole is restrained by the second blocking portion. In the second state, the third tube can be moved from the second blocking portion to a position that is other than a second opening portion and in which a third flow path of the third tube is reopened.

A blood bag system includes a BC pooling bag for centrifugation of a buffy coat, a filter for removing white blood cells from a supernatant liquid transferred from the BC pooling bag, a platelet preserving bag for reserving the supernatant liquid that has passed through the filter, a first tube connecting the BC pooling bag and an inlet of the filter, a second tube connecting the platelet preserving bag and an outlet of the filter, and a cassette to be fixed in the centrifugation and separation apparatus. The first tube and the second tube are disposed within the cassette. The cassette includes a first clamp section for closing and opening the first tube, and a second clamp section for closing and opening the second tube.

A system and method are provided for separating previously-collected whole blood into a red blood cell fraction and a plasma fraction by which the container of previously-collected whole blood is determined to be empty based on using the combination of the measured gross weight of the container and a calculated fluid flow rate from the container, based on weigh scale feedback. Upon detection of the empty container, flow from the container is stopped.

Provided is a blood filter that resists deterioration in properties as a result of electron beam sterilization treatment performed before or during use as a blood filter, has durability, dimensional stability, and chemical resistance at excellent levels, also has biocompatibility, and resists deterioration in properties even upon the electron beam sterilization treatment. The blood filter according to the present invention includes a nonwoven fabric made of PEEK fibers. Preferably, the blood filter according to the present invention has an average pore size of 3 to 280 μm and has a porosity of 15% to 70%; and the PEEK fibers have an average fiber diameter of 10 μm or less.

A method for the re-anticoagulation of platelet rich plasma in a blood apheresis system includes priming the blood apheresis system with anticoagulant, such that a volume of anticoagulant is transferred to a PRP container. The method may then transfer the anticoagulant within the PRP container to a red blood cell container, and collect a volume of platelet rich plasma within the PRP container. The platelet rich plasma may be collected in a plurality of cycles. Between collection cycles, the method may transfer a portion of the volume of anticoagulant from the red blood cell container to the PRP container.

Flexible housing filters for filtration of fluids and methods of making such filters are disclosed. The filters may include one or more peripheral seals in the flexible housing.

Systems and methods for the washing and processsing of biological fluid/biological cells are disclosed. The systems and methods prevent inadvertent target cell loss by monitoring pressure and providing for the dilution of the cell feed.