A method of analysis using mass spectrometry and/or ion mobility spectrometry is disclosed. The method comprises: using a first device to generate smoke, aerosol or vapour from a target comprising or consisting of a microbial population; mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data; and analysing said spectrometric data in order to analyse said microbial population.

Systems and methods are disclosed for utilizing an ion mobility cell to improve desolvation prior to interaction with a hydrogen-deuterium exchange reagent, thereby improving the accuracy of the HDX data generated by MS and reducing the effects of conformational changes that can occur with increased temperatures.

Systems and methods are disclosed for utilizing an ion mobility cell to improve desolvation prior to interaction with a hydrogen-deuterium exchange reagent, thereby improving the accuracy of the HDX data generated by MS and reducing the effects of conformational changes that can occur with increased temperatures.

A method for removing deposits in a mass spectrometer ion source housing includes delivering a liquid from a liquid source to a surface within the ion source housing. The surface including an ultrasonic transducer embedded within the surface. The method further includes activating the ultrasonic transducer to ultrasonically remove the deposit.

A connector for use in vacuum system is configured to fluidly connect a first opening formed in a first vacuum chamber to a second opening formed in a second vacuum chamber, the first opening of the first vacuum chamber being provided within the second vacuum chamber. The connector comprises a tube and a biasing O-ring. The tube has an outer wall to define a fluid flow path between first and second ends of the tube. Towards the first end, a sealing portion of the outer wall of the tube is provided. Towards the second end, an O-ring retaining point is provided along the tube spaced apart from the second end of the tube. The biasing O-ring is provided around and tensioned by the outer wall of the tube. The biasing O-ring is moveable along the axial direction between the O-ring retaining point and a sealing position where it seals the connector.

A connector for use in vacuum system is configured to fluidly connect a first opening formed in a first vacuum chamber to a second opening formed in a second vacuum chamber, the first opening of the first vacuum chamber being provided within the second vacuum chamber. The connector comprises a tube and a biasing O-ring. The tube has an outer wall to define a fluid flow path between first and second ends of the tube. Towards the first end, a sealing portion of the outer wall of the tube is provided. Towards the second end, an O-ring retaining point is provided along the tube spaced apart from the second end of the tube. The biasing O-ring is provided around and tensioned by the outer wall of the tube. The biasing O-ring is moveable along the axial direction between the O-ring retaining point and a sealing position where it seals the connector.

The invention relates to a vacuum system, comprising a vacuum pump, preferably turbomolecular pump, and at least one vacuum chamber, wherein the vacuum pump comprises: at least a first and a second inlet and a common outlet; at least a first and a second pumping stage, each pumping stage comprising at least one rotor element being arranged on a common rotor shaft, wherein the first inlet is connected to an upstream end of the first pumping stage and the second inlet is connected to an upstream end of the second pumping stage; a direction element for preventing a gas flow from a downstream end of the first pumping stage to the second inlet; a conduit having a conduit inlet and a conduit outlet, wherein the conduit inlet is connected to the downstream end of the first pumping stage and the conduit outlet is connected to a location downstream of the second pumping stage; wherein the first inlet and the second inlet of the pump are connected to the same vacuum chamber.

A mass spectrometry method comprises: (a) introducing ions and gas into a first electrode section of an ion transport apparatus along an axis, the ion transport apparatus further comprising a second electrode section including: a plurality of stacked, mutually parallel ring or plate electrodes; and an ion outlet aperture configured to receive the ions from the second electrode section and to transfer the ions to the vacuum chamber; (b) providing only non-oscillatory voltages to electrodes of the first electrode section of the ion transport apparatus that divert motion of the ions away from that axis and towards an entrance aperture of the second electrode section; (c) transporting the ions through the second electrode section to and through the ion outlet aperture to the vacuum chamber; and (d) removing a major portion of the gas through an exhaust port that is offset from the ion outlet aperture.

A mass spectrometry method comprises: (a) introducing ions and gas into a first electrode section of an ion transport apparatus along an axis, the ion transport apparatus further comprising a second electrode section including: a plurality of stacked, mutually parallel ring or plate electrodes; and an ion outlet aperture configured to receive the ions from the second electrode section and to transfer the ions to the vacuum chamber; (b) providing only non-oscillatory voltages to electrodes of the first electrode section of the ion transport apparatus that divert motion of the ions away from that axis and towards an entrance aperture of the second electrode section; (c) transporting the ions through the second electrode section to and through the ion outlet aperture to the vacuum chamber; and (d) removing a major portion of the gas through an exhaust port that is offset from the ion outlet aperture.

Apparatus, systems, and methods disclosed herein utilize a lid for closing, substantially sealing, and providing an electrical interface for mass spectrometry systems. In various aspects, the present disclosure provides a lid having a plurality of layers with electrical connections therethrough. In some embodiments, lids for use in a mass spectrometry system as are disclosed herein can include a plurality of layers having electrical connections such that their inputs and outputs are laterally staggered across these layers. In some embodiments, the present disclosure provides methods of making and using disclosed lids and mass spectrometry systems. In some embodiments, implementations of the present disclosure are useful in mass spectrometry systems, including, for example, improving and simplifying assembly.