Various embodiments disclosed relate to increasing the proportion of omega-3 fatty acid in seed oil produced by a plurality of Brassica plants, such as canola, transgenically modified to produce seed oil comprising at least one of EPA, DHA and DPA. Transgenic Brassica plants, such as transgenic canola, are subjected to an environment which has an average daily day-night temperature difference of at least 13° C. during the transgenic plant's period of seed maturation. The seed oil is at least 5 wt % EPA. The seed oil is at least 1 wt % DPA. The seed oil is at least 0.2 wt % DHA. The seed oil is at least 5.2 wt % a mixture of EPA and DHA. The seed oil is at least 6 wt % long chain omega-3 fatty acids.

Various embodiments disclosed relate to increasing the proportion of omega-3 fatty acid in seed oil produced by a plurality of Brassica plants, such as canola, transgenically modified to produce seed oil comprising at least one of EPA, DHA and DPA. Transgenic Brassica plants, such as transgenic canola, are subjected to an environment which has an average daily day-night temperature difference of at least 13? C. during the transgenic plant's period of seed maturation. The seed oil is at least 5 wt % EPA. The seed oil is at least 1 wt % DPA. The seed oil is at least 0.2 wt % DHA. The seed oil is at least 5.2 wt % a mixture of EPA and DHA. The seed oil is at least 6 wt % long chain omega-3 fatty acids.

The disclosure relates to CYP710A genes associated with enhanced healthy sterols in plants. Also disclosed are plants comprising altered expression or activity of the CYP710A genes along with related methods for enhancing healthy sterols in plants. Also provided are oils and compositions containing altered sterol levels.