Described herein are embodiments relating to manipulation of populations and sex ratio in populations through DNA sequence modifications.

Provided is a gene expression system, suitable for expression in an insect, comprising an insect muscle actin promoter operably linked to a marker gene, which overcomes or ameliorates one or more of: cost of rearing; amount of handling; errors in identification due to human error or loss of marker by the insect; and health concerns related to the effects of marker powders on workers in mass rearing facilities.

A method of producing a transgenic silkworm that spins bagworm silks and producing a large quantity of bagworm silks by transgenic technology is developed and provided. A gene encoding a modified bagworm Fib H and a transgenic silkworm in which the gene is introduced, wherein the gene is obtained by cloning a gene fragment encoding a bagworm Fib H-like polypeptide comprising a partial amino acid sequence of bagworm Fib H, and fusing the gene fragment to a gene fragment encoding silkworm-derived Fib H, are provided.

One variation of a method for producing a target compound includes: during an initial period, genetically modifying a population of insects to produce a target compound; during a growth period succeeding the initial period, cultivating the population of insects; during a treatment period succeeding the growth period, applying a dosage of a stressor to the population of insects, the stressor configured to trigger production of the target compound; in response to a proportion of the target compound within the population of insects exceeding a threshold proportion, harvesting the population of insects; homogenizing the population of insects to form a blend comprising the proportion of the target compound and a second proportion of a set of secondary components; and separating the proportion of the target compound from the second proportion of the set of secondary components for extraction of the proportion of the target compound from the blend.

An automated process for breeding insects at their imaginal stage in at least one mating cage, comprises the steps of: providing at least one emergence chamber comprising a population of newly emerged insects; automatically successively: connecting at least one emergence chamber with at least one mating cage, filling the at least one mating cage with at least part of the population of newly emerged insects during a first time interval T1, and disconnecting the at least one mating cage and the at least one emergence chamber; providing conditions within the at least one mating cage suitable for promoting ovipositing during a second time interval T2; stopping ovipositing during a third time interval T3.

The disclosure relates to genetically modified bacteria, genetically modified arthropods, and methods for controlling and/or reducing arthropod populations.

Variable-scale, modular, easily manufacturable, energy efficient, reliable, and computer operated Insect Production Superstructure Systems (IPSS) and Farming Superstructure Systems (FSS) may be used to produce cannabis, insects, and psilocybin mushrooms for human and animal consumption, and for the extraction and use of lipids, drugs, and chemicals for applications involving medicine, nanotechnology, consumer products, pharmaceuticals, pet food, and chemical production with minimal water, feedstock, and environmental impact.

The invention relates, in part, to methods to design and construct gene drives such as daisy chain gene drives, suppression gene drives, and other types of gene drives that may be included in cell lines and organisms.

The disclose relates to genetically modified bacteria, genetically modified arthropods, and methods for controlling and/or reducing arthropod populations.

Disclosed is the use of genetic means or a related inhibitor to inhibit and down-regulate the amount of PI4KIII protein, RBO/EFR3/EFR3A/EFR3B membrane proteins, TTC7 protein and membrane protein complexes formed by said proteins, or related enzyme activity to promote A secretion by neuronal cells, reduce A accumulation within neurons, and thereby reduce AD model fruit fly and mouse nerve dysfunction.