This disclosure relates to methods of preserving mesenchymal stromal/stem cells (MSCs) for use in clinical applications. In certain embodiments, this disclosure relates to methods of preserving MSCs comprising mixing MSCs with interferon-gamma prior to cryopreserving, freezing, or cooling the MSCs to a temperature below zero degrees Celsius.

Relates to a method for pathogen reduction of a blood product comprising: i) removing oxygen from a blood product; ii) reducing blood pathogens from said blood product t: adding amustaline (S-303) to a final concentration of 0.2 millimolar (mM); and glutathione (GSH) to a concentration of 20 mM; iii) reducing S-303 to a concentration of less than 1 nmol/L comprising incubating said oxygen reduced blood product under oxygen reduced conditions for up to 6 hours; and further relates to a pathogen reduced, oxygen reduced blood product having an oxygen saturation (sO.sub.2) of less than 25%, a pCO.sub.2 of 90 mmHg or less at 37° C., and having a amustaline (S-303) concentration of less than 1 nmol/L.

The present invention provides methods for cryopreservation of insect embryos comprising chorion layers and waxy layer, particularly fly embryos including embryos of Black Soldier Fly (Hermetia illucens, BSF) useful in mass rearing of beneficial fly adults. The present invention further provides cryopreserved embryos having an industrially suitable adult recovery and survival rate.

The invention relates to a method for increasing the lifespan of animal sperm comprising contacting said sperm with an inhibitor of Slo3 potassium channel. The invention also relates to a use of an inhibitor of Slo3 potassium channel, for increasing the lifespan of animal sperm or motility of capacitated animal sperm, comprising contacting an inhibitor of Slo3 potassium channel with said sperm. Moreover, the invention relates to an artificial insemination instrument for use in artificial insemination of an animal, comprising animal sperm in contact with an inhibitor of Slo3 potassium channel. The invention also relates to a method for artificially inseminating an animal using said artificial insemination instrument. Eventually, the invention relates to a method for increasing the fertility of an animal, comprising contacting sperm of said animal with an inhibitor of Slo3 potassium channel; then artificially inseminating said animal with said sperm.

Compositions and Methods for Stabilizing Circulating Tumor Cells Methods and compositions for stabilizing a biological sample for analysis, comprising the steps of obtaining in a sample collection device a biological sample from a subject, especially blood, the biological sample including at least one circulating tumor cell from the subject. The methods may include a step of contacting the biological sample with a protective agent composition that includes a preservative agent, an optional anticoagulant, and a quenching agent to form a mixture that includes the protective agent composition and the sample.

Members of the PrC-210 family of aminothiols, including PrC-211 and PrC-252, are shown to be highly effective in reducing ischemia-reperfusion injury in two preclinical models, including kidney transplant and myocardial infarct. Compositions and methods employing members of the PrC-210 family of aminothiols are disclosed for suppressing ischemia-reperfusion-induced cell and organ toxicities in a number of settings, significantly including organ transplant and myocardial infarct.

A composite material film for expanding circulating tumor cells ex vivo and a preparation method thereof, a kit and a method for expanding circulating tumor cells ex vivo, a method for detecting an effect of a drug, and a cryopreservation solution are provided. The preparation method includes: mixing one or more kinds of particles and a solvent to form a mixed liquid, in which the particles are selected from the group consisting of metal particles, metal oxide particles, silicon oxide particles and combinations thereof; placing the mixed liquid on a substrate to form a particle layer; adding a medium material to the particle layer, in which the medium material is selected from the group consisting of styrene and its derivatives, polyester monomers, silicon oxide compounds and combinations thereof; and polymerizing the medium material to form a medium layer to fix the particle layer on the substrate.

Among the various aspects of the present disclosure is the provision of a media for cryopreserved cells and methods of making and using same. An aspect of the present disclosure provides for a cell media formulation comprising (e.g., for day 0) one or more components selected from: MCDB 131; Glutamax; P/S; BSA; Glucose; ZnSO4; an enzyme for digesting DNA (e.g., DNASE1); and/or an apoptosis inhibitor (e.g., BI-6C9).

Compositions, devices, and systems comprising a corneal tissue carrier, wherein the corneal tissue carrier comprises a corneal tissue sample and a fluid; wherein the fluid comprises resazurin. Additionally, methods including measuring cell viability.

The disclosure provides for cryopreservation compositions and methods of use thereof. Aspects of the present disclosure provide for cryopreservation compositions useful in the freezing and thawing of cell therapies. Other aspects of the present disclosure provide for systems for use in the cryopreservation cell therapies. Still other aspects of the present disclosure provide for methods of cryopreserving cell therapies.