The present invention relates to a method for vitrification and thawing of oocytes of animals for somatic cell cloning. More specifically, the present disclosure relates to a method for vitrification and thawing of canine oocytes, and to thus produced frozen-thawed oocytes. In a conventional approach of the vitrification-frozen oocyte production for the dog, an estrous cycle may not coincide with an experimental schedule. However, the method for vitrification and thawing of the canine oocyte according to the present disclosure and the resulting frozen-thawed oocyte allows an experimental schedule to coincide with the estrous cycle, resulting in high nuclear transfer and fertilization effects.

There is disclosed a cryo-carrier comprising a carrier region having a plurality of grids, the carrier region having an embryo placement portion for supporting at least one embryo and a non-embryo placement portion surrounding the embryo placement portion. The non-embryo placement portion is filled with a support sheet so that it is stronger than the embryo placement portion, thereby supporting it.

A biological specimen of a subject is handled and tracked for a procedure involving that specimen. Prior to initiation of the procedure, a first procedure data structure (PDS) is generated. The first PDS binds an identifier corresponding to the subject with an indicator of a procedure to be performed on the specimen and identifiers of a specimen container and a specimen holder that physically contacts the biological specimen, as well as a scheduled time for the procedure. A schedule of a plurality of PDSs including the first PDS, is displayed on a display device of a graphical user interface. Following initiation of the procedure, the first PDS is updated based on user input, and after the procedure, at least a portion of the first PDS, as updated, is stored in a database in conjunction with other PDSs respectively associated with other completed procedures.

A liquid nitrogen tank includes a tank, a storage rack and a drive component. The tank includes a tank cover, a tank body, a vacuum cavity layer and a heat-insulating cavity layer. The tank cover is disposed to cover on the tank body. An access door is provided on the tank body. A storage cavity is provided in the tank body. The heat-insulating cavity layer is provided on a periphery of the storage cavity. The vacuum cavity layer is provided on a periphery of the heat-insulating cavity layer. The storage rack is provided in the storage cavity. A plurality of cryopreservation tube racks are stored in the storage rack. The drive component can drive the storage rack to rotate and move up and down in the storage cavity, and can drive the plurality of cryopreservation tube racks to move to a position corresponding to the access door.

The invention relates to a device for the temperature monitoring of a cryopreserved biological sample, comprising a sample container having an accommodating space (2) for accommodating the sample and comprising an indicating apparatus, which can be arranged on the outside of the sample container, for monitoring at least one temperature limit value. The indicating apparatus has at least one cavity, which is only partially filled with an indicating substance, the melting temperature of which lies in a range from −20 ° C. to −140 ° C. The indicating apparatus can be designed, in particular, as a cylindrical body, which can be fastened to a cryotube as a bottom part, or alternatively as a double-walled hollow cylinder, which can be slid onto an outer lateral surface of the cryotube. The indicating apparatus can also be fastened to a lateral outer wall of the sample container, e.g. as a hollow body that can be inserted into a sleeve or insertion pocket.

Embodiments of the present invention may provide effective processing of materials through phase transitions with a mobile phase transition device which may have a frozen storage area and a thawing area and which can be used to thaw biological materials near a recipient of the materials. A mobile phase transition device may be automated so that the thawing of materials can precisely follow thawing protocols.

A vitrification device for gametes or embryos, wherein, the vitrification straw comprises: a loading rod, wherein the loading rod is a metal rod; a loading strip, wherein, the loading strip is connected with one end of the loading rod. According to the present invention, the loading rod is arranged as metal rod, which avoids embrittlement fracture caused by sudden temperature change when the loading rod is taken out of liquid nitrogen, moreover, the ice crystals that form on gametes or embryos when the loading rod floats out of the surface of liquid nitrogen, affecting the safety of gametes or embryos, the metal material used by this invention can increase the weight of the loading rod, preventing it from floating up in the liquid nitrogen, hence improve safety of gametes or embryos.

Vial assemblies comprise a tubular body and a cap, the cap including a first portion configured to abut a lip of an open end of the tubular body, a threaded portion configured to couple to threading on an internal surface of the tubular body, and a second portion protruding from the threaded portion and extending into a cavity of the tubular body. Methods for storing and removing frozen samples from such vial assemblies are also described.

A system for cryocooling biological samples, including a first chamber configured to hold a first amount a cryogenic liquid; a container holder positioned in thermal contact with the first chamber and configured to hold at least one removable container positioned therein, wherein the container is configured to hold a second amount of the cryogenic liquid and forms a second chamber; an elongated tube holder configured to hold at least one hollow elongated tube into the container; and a sample wand configured to hold and transfer at least one sample holder with a biological sample into the elongated tube while the elongated tube is in the container with the second amount of the cryogenic liquid therein.

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.