This disclosure relates to scale-down systems for freezing and thawing aqueous liquid mixtures of thermo sensitive substances. In at least one aspect, the system can comprise a first container that has a head-space and a liquid-space, with a substantially constant average transversal cross-section area in the liquid-space that does not change substantially with height. The first container holds a volume of liquid for a height of liquid, has a lateral active heat transfer area having an insulated lateral area ALIM, and has an equivalent radius sized to reproduce the equivalent radius of a second container. The first container further has a lateral heat transfer area in contact with the liquid-space. The first container is smaller than the second container, and the insulated lateral area is equal to or greater than the lateral active heat transfer area.

A system for testing the closure integrity of a sealed container at a cryogenic temperature is provided. The system includes a sealed container containing helium having an opening sealed by a closure, a cryogenic storage vessel at least partially filled with a cryogenic fluid at the cryogenic temperature, a hollow tube extending through an opening of the cryogenic storage vessel, a fixture configured to engage the sealed container, and a leak detection unit connected to the hollow tube. The hollow tube has a first end positioned outside of the cryogenic storage vessel and an opposing second end positioned inside the cryogenic storage vessel. The fixture is configured to be removably inserted within the hollow tube through an opening at the first end of the hollow tube, such that the sealed container is positioned at or proximate the second end of the hollow tube.

The present technology generally relates to a cryogenic tissue sample storage system. Select embodiments of a tissue sample storage system include a tray having a first well configured to receive a first tissue sample therein a second well configured to receive a second tissue sample therein. The tray can include a first well identification feature associated with the first well and a second well identification feature associated with the second well. The storage system can further include a lid hingedly associated with the tray and movable between (a) a first position in which the first and second wells are occluded by the lid, and (b) a second position in which the first and second wells are externally accessible. The first well identification feature can be unique from the second well identification feature.

A carrier for a storage bag is disclosed. The carrier includes a base and a lid that define an interior region in a closed configuration. The base and lid are configured to retain the storage bag and tubes of the storage bag within the interior region in the closed configuration. A distance between the base and lid in the closed configuration is adaptable between a contacting position and a floating position to allow for maximum contact of the storage bag with the base and the lid.

A storage bag (100) includes a main tube (102) extending from a first end to a second end and defining a first inner surface and outer surface. The main tube includes a first angled edge (128) and a second angled edge (132) extending from the first end of the main tube and forming a first angled interface. The storage bag also includes a port tube (104) extending from a first end to a second end and defining a second inner surface and outer surface. The second end of the port tube includes a third angled edge and a fourth angled edge extending from the second end of the port tube, forming a second angled interface. The second angled interface of the port tube is positioned over the first angled interface of the main tube. A portion of the port tube not overlapping the main tube is sealed to itself to form an angled drainage area (182).

Examples of a cryopreservation device (200, 300) for storing reproductive biological material are described herein. The device (200, 300) includes a vitrification straw (202, 302) having an elongated member (208, 306) with a planar base and a triangular cross-section. The vitrification straw (202, 302) further includes a tip member (208, 308) extending away from one end of the elongated member (208, 306). At least a portion of the tip member (208, 308) defines a cavity (212) to receive the biological material. Further, the cryopreservation device includes a sheath (204, 304, 402) for being disposed over the tip member (208, 308) of the vitrification straw (202, 302).

A cell cryopreservation pretreatment operation plate includes a planar operation portion, a first recess for storing a treatment solution, a second recess for storing a treatment solution, a third recess for storing a treatment solution, and a fourth recess for storing discarded solutions. The planar operation portion includes, on a surface thereof, a first circular segment, second circular segment, and third circular segment for placing treatment solutions, an enlarged first circular segment enclosing the first circular segment, an enlarged second circular segment enclosing the second circular segment, and an enlarged third circular segment enclosing the third circular segment.

An improved packaging assembly for storing, distributing, treating, mixing, and dispensing tissue and/or cellular material and/or implantable material. The packaging assembly may include pouches, tubes, and a bag made of a sealable, flexible polymeric material that is open at one end and a needle-free swabable connector attached to the pouch at the other end and acting as a port to allow for the introduction or discharge of biological solutions, rinsing solution, and/or preservation solutions into the packaging assembly. The designed thickness of the wall of the packaging assembly facilitates efficient heat/cold transfer, which is useful for successful controlled rate freezing, quick thawing, and resuscitation of viable cells or tissue. The packaging assembly is also useful for combining additional biological fluids with the cellular material or tissue, and for efficient mixing of the biological fluids with the tissue and/or cellular material in the assembly.

A system for cryocooling biological samples includes a first chamber that holds a first amount a cryogenic liquid. A container holder, which is positioned in thermal contact with the first chamber, holds a removable container positioned therein. The container holds a second amount of the cryogenic liquid and forms a second chamber. An elongated tube holder holds a hollow elongated tube into the container. A sample wand holds and transfers a sample holder with a biological sample into the elongated tube while the elongated tube is in the container with the second amount of the cryogenic liquid.

The present invention relates to a method for cryopreserving and thawing an organ-on-a-chip that has a three-dimensional tissue structure and function. Specifically, the present invention relates to a method for cryopreserving and thawing an organ-on-a-chip that comprises cells and hydrogel and has a microchannel structure, whereby the organ-on-a-chip has an excellent effect of being able to maintain the structure and function of the three-dimensional tissue before and after cryopreservation and thawing.