The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.

Microbiota restoration therapy compositions and methods for manufacturing, processing, and/or delivering microbiota restoration therapy compositions are disclosed. An example method for manufacturing a microbiota restoration therapy composition may include collecting a human fecal sample and adding a diluent to the human fecal sample to form a diluted sample. The diluent may include a cryoprotectant. The method may also include mixing the diluted sample with a mixing apparatus and filtering the diluted sample. Filtering may form a filtrate. The method may also include transferring the filtrate to a sample bag and sealing the sample bag.

The disclosure relates to devices, solutions and methods for collecting and processing samples of bodily fluids containing cells (as well as embodiments for the collection, and processing and/or analysis of other fluids including toxic and/or hazardous substances/fluids). In addition, the disclosure relates generally to function genomic studies and to the isolation and preservation of cells from saliva and other bodily fluids (e.g., urine), for cellular analysis. With respect to devices for collection of bodily fluids, some embodiments include two mating bodies, a cap and a tube (for example), where, in some embodiments, the cap includes a closed interior space for holding a sample preservative solution and mates with the tube to constitute the (closed) sample collection device. Upon mating, the preservation solution flows into the closed interior space to preserve cells in the bodily fluid. The tube is configured to receive a donor sample of bodily fluid (e.g., saliva, urine), which can then be subjected to processing to extract a plurality of cells. The plurality of cells can be further processed to isolate one and/or another cell type therefrom. The plurality of cells, as well as the isolated cell type(s), can be analyzed for functional genomic and epigenetic studies, as well as biomarker discovery.

Systems and methods of the invention generally relate to prolonging viability of bodily tissue, especially lung tissue, through the use of an expandable accumulator to maintain a constant pressure within the lumen of the organ even during external pressure fluctuations due to, for example, flight. Systems and methods may include prolonging donor organ viability in storage through the use of an organ container that mimics the geometry and orientation of the organ in vivo.

A Descemet Membrane Endothelial Keratoplasty (DMEK) holder for placement within a vial for supporting a corneal tissue inserter is provided. The DMEK holder includes a top and an opposite bottom, at least one support member extending between the top and the bottom, with a bottom portion of the at least one support member sized and shaped to fit against a bottom inner surface of the vial to thereby support the corneal tissue inserter within the vial, and a first annular member having an opening sized and shaped such that a bottom end of the corneal tissue inserter is insertable through the opening and the first annular member separates the corneal tissue inserter from an inner surface of the vial and positions the corneal tissue inserter within the vial away from inner surfaces of the vial to thereby improve retrieval of the corneal tissue inserter by a surgeon.

The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.

Manual and automated methods of pre-charging an empty or partially empty insulated container with CO2 snow are provided. A first location such as a charging location charges CO2 liquid into a container to create a pre-charged container with CO2 snow. The charging location prepares the pre-charged container for delivery to a second location, either by itself, or through a third party. The second location may be a clinical site, which upon receipt of the pre-charged container, loads a perishable item such as a biological sample into the pre-charged container. A user receives the pre-charged container with perishable item and removes the perishable item from the pre-charger container for testing (e.g., biological testing). Depending on the level of depletion of the CO2 snow in the pre-charged container, the user returns the depleted container to the first location or the intermediate location.

Assemblies for storing, handling, transporting, viewing, evaluating, and/or shipping corneal tissue are provided. The assembly includes a corneal tissue carrier, optimized for DSAEK and UT-DSAEK corneal grafts, within a transport vial, the transport vial removably coupled to a stabilization base, wherein the ease of access to the graft carrier allows administering the corneal tissue sample to a patient in rapid succession so that more surgeries can be performed by a single surgeon in a single day.

The present application provides a unique way for storing biomaterials, blood, blood products, vaccines, organs in an organ and fluid preservation and transportation system. The organ and fluid preservation and transportation system can comprise a docking system, a container system comprising an upper container, an inner container, an outer container, electronics, and a heating/cooling module. The upper container can attach to the outer container, and/or inner container and create an airtight seal with the environmental conditions staying consistent when sealed wherein the container system can be docked to the docking system wherein the docking system heats or cools down the container system.

A protector assembly for handling, storing and transporting donor tissue in a transfer pipette includes a distal cap and a proximal plug that are respectively engageable with opposite ends of the pipette. Structurally, the pipette includes a distally tapered fluid chamber which is in fluid communication with an extension tube. The distal cap is dimensioned for friction engagement with the fluid chamber of the pipette and it is formed with a plurality of vents to establish fluid communication through the vents with the fluid chamber. On the other hand, the proximal plug is dimensioned for a friction engagement with the extension tube to prevent leakage of solution from the fluid chamber of the pipette when the proximal plug is engaged with the pipette. In combination, an engagement of the protector assembly with a pipette holding a donor tissue will protect and preserve the donor tissue during handling and transport.