Disclosed herein are devices, apparatus, systems, methods and kits for collecting and storing a fluid sample from a subject. A device for collecting the fluid sample can include a housing comprising a recess having an opening, a vacuum chamber in the housing and in fluidic communication with the recess, and one or more piercing elements that are extendable through the opening to penetrate skin of the subject. The vacuum chamber can be configured for having a vacuum that draws the skin into the recess. The recess can be configured having a size or shape that enables an increased volume of the fluid sample to be accumulated in the skin drawn into the recess.

Provided are methods of detecting a marker for active tuberculosis, and a portable device for carrying out a method of detecting a marker for active tuberculosis. Also provided is a system for carrying out a method of detecting a marker for active tuberculosis, a method for pre-treating a sample stream from a human or animal suspected of having active tuberculosis, a system for pre-treating a sample stream from a human or animal suspected of having active tuberculosis and kits for performing said methods.

A capillary chamber for performing chemical and physical analysis using a plurality of whole blood samples is presented. Following a loading of the whole blood samples into a plastic tube, the whole blood samples are distributed into a collection chamber and the capillary chamber has means of adding respective vaccines or similar biological products into each capillary tubes. The whole blood is then analyzed using standard microscopic techniques with sufficient resolution and contrast to record the chemical and physical properties of the blood immediately after withdrawal.

A syringe-based device includes a housing, the housing including a port, and an actuator mechanism including a first member and a second member configured to be movably disposed within the housing. The first member includes a syringe body and a plunger movably disposed within the syringe body and including a first plunger seal. The second member includes a second plunger seal defining a channel and a valve fluidically coupled with the channel of the second plunger seal. The device also includes a first fluid reservoir and a second fluid reservoir. The device transitions from a first configuration to a second configuration in which a first type of fluid in the first fluid reservoir is delivered through a port, to a third configuration in which a second type of fluid within the second fluid reservoir is delivered through the port.

A mobile, self contained molecular diagnostics system is provided with a microfluidic chip, detection apparatus and an integrated or wireless control interface and imager. The system provides automated sample preparation and rapid optical detection of multianalyte nucleic acids and proteins. On chip PCR may be performed to improve the optical fluorescence signal for nucleic acid detections. Plasmonic protein detection is performed using a dark field smartphone microscope. Dark field illumination is based on an evanescent field generated by LED total internal reflection. The smartphone element may also be used as an interface to control the detection apparatus, acquire images, process data and for wireless communications with remote computers. The handheld automated system has low power requirements and is particularly suited for point of care and on demand diagnostics in resource limited settings.

A blood collection apparatus is provided with a blood collection assembly including a casing tube including a forward nose, a plurality of connectors and a rear flange having a flexible projection, a plunger slidably disposed in the casing tube and including a forward plug and an elongated arm having a plurality of cavities, a blood collection tubes, an adapter assemblies each including a tube body, a first tube extending out of one end and insert into a related connectors of the casing tube, a second tube extending out of the end to insert into one of the blood collection tubes, and a butterfly needle assembly mounted to the nose of the casing tube. A pulling of the plunger moves the plug rearward and pivots the projection to engage in one cavity of the elongated arm and an orifice of the each related connectors is not blocked by the plug so as to flow blood from the tube casing to one of the blood collection tube via one connector and one adapter assembly.

A fluid control device includes an inlet configured to be placed in fluid communication with a bodily fluid source and an outlet configured to be placed in fluid communication with a fluid collection device, which can produce a negative pressure differential between the outlet and the inlet. A sequestration portion is in fluid communication with the inlet and includes a first flow controller configured to transition from a first state to a second state to place the sequestration portion in fluid communication with the outlet when the negative pressure differential has a first magnitude. A sampling portion is in fluid communication with an outlet and includes a second flow controller configured to transition from a first state to a second state to place the sampling portion in fluid communication with the inlet when the negative pressure differential has a second magnitude greater than the first magnitude.

A system acquiring body fluid samples is provided. One embodiment comprises a housing with a sampling channel extending therethrough and defined by an inlet port and an outlet port, wherein the inlet port secures a needle that penetrates a patient; a diversion chamber body portion directly secured to the housing with at least one diversion chamber, wherein the diversion chamber is in fluid communication with the sampling channel, wherein an initial first portion of the body fluid received from the needle is transferred through the sampling channel inlet port and into the diversion chamber, and wherein a subsequent second portion of the body fluid received from the needle is communicated through the sampling channel to the outlet port for collection into a collection device while the initially received first portion of the body fluid is retained in the diversion chamber.

A system acquiring body fluid samples is provided. One embodiment comprises a housing with a sampling channel extending therethrough and defined by an inlet port and an outlet port, wherein the inlet port secures a needle that penetrates a patient; a diversion chamber with a partial vacuum, wherein the diversion chamber is in fluid communication with the sampling channel, wherein an initial first portion of the body fluid received from the needle is transferred through the sampling channel inlet port and into the diversion chamber, and wherein a subsequent second portion of the body fluid received from the needle is communicated through the sampling channel to the outlet port for collection into a collection device while the initially received first portion of the body fluid is retained in the diversion chamber.

An apparatus includes a housing, defining an inner volume, and an actuator mechanism movably disposed therein. The actuator mechanism is configured to be transitioned from a first configuration to a second configuration to define a pre-sample reservoir fluidically couplable to receive a pre-sample volume of bodily-fluid via an inlet port of the housing. The actuator mechanism is movable from a first position to a second position within the housing after the pre-sample reservoir receives the pre-sample volume such that the housing and the actuator mechanism collectively define a sample reservoir to receive a sample volume of bodily-fluid via the inlet port. The outlet port is in fluid communication with the sample reservoir and is configured to be fluidically coupled to an external fluid reservoir after the sample volume is disposed in the sample reservoir to transfer at least a portion of the sample volume into the external fluid reservoir.